Microcallus formation from leaf mesophyll protoplasts in the genus Actinidia Lindl.

Abstract:

:Microcallus (more than 60 cells) formation was obtained from leaf mesophyll protoplasts of 6 species and varieties in the genus Actinidia Lindl. (kiwifruit). The best results were achieved by using liquid over agarose culture for A. arguta var. arguta, liquid and agarose disc type culture for A. arguta var. purpurea, agarose disc type culture for A. arguta cv. Issaï and A. deliciosa and liquid agarose bead type- and disc type culture for A. kolomikta and A. polygama. Several factors influencing purification, browning, survival and sustained division of the protoplasts are briefly discussed.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Xiao XG,Hirsch AM

doi

10.1007/BF00231583

subject

Has Abstract

pub_date

1996-09-01 00:00:00

pages

896-9

issue

12

eissn

0721-7714

issn

1432-203X

journal_volume

15

pub_type

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