Abstract:
:A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protoplasts by floating them up into a 0.5 M sorbitol layer. Regeneration occurred over a period of 10-24 days, and, under optimal osmotic conditions, followed the developmental pattern seen during spore germination, in that the first division gave rise to a primary rhizoid. Thus, prothallial protoplasts are comparable to germinating spores as suitable models for studies of developmental polarity in single cells. As in germinating spores, the polarity of development in regenerating protoplasts is influenced by the vectors of gravity and unilateral light. However, the relative influence of light in fixing this polarity is greater in regenerating protoplasts, while in germinating spores, the influence of gravity is greater.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Edwards ES,Roux SJdoi
10.1007/s002990050470keywords:
subject
Has Abstractpub_date
1998-06-01 00:00:00pages
711-6issue
9eissn
0721-7714issn
1432-203Xjournal_volume
17pub_type
杂志文章abstract::An in vitro propagation technique based on axillary bud proliferation has been developed for matureSapium sebiferum trees. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (1-10 μM and α-naphthaleneacetic acid (0-0.5 μM showed axillary bud proliferation. Shoots proliferated i...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275506
更新日期:1997-06-01 00:00:00
abstract::A biolistic transformation procedure was used to transform embryogenic Pinus radiata tissue with constructs containing the Zea mays UBI1 (ubiquitin)-promoter followed by the P. radiata CAD (cinnamyl alcohol dehydrogenase) cDNA in sense or anti-sense orientation or in the form of an inverted-repeat. The effect of the d...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0911-y
更新日期:2005-05-01 00:00:00
abstract::Hypocotyl explants of 1 and 10 mm lengths were excised from 12-day-old in vitro-grown seedlings of Albizia richardiana. The larger pieces, after 40 days of culture, developed shoots along with green calli on B5 + BAP (10(-7)-10(-5)M), while the smaller segments produced only green calli on B5+BAP (10(-7)-10(-4)M) medi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272982
更新日期:1988-01-01 00:00:00
abstract::A differential expression study on the Saccharum spp.-Sporisorium scitamineum pathogenic interaction was carried out in the susceptible Ja60-5 and the resistant M31/45 genotypes. Using cDNA-AFLP analysis, a total of 64 transcript-derived fragments (TDFs) was found to be differentially expressed, with the majority (67....
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0524-y
更新日期:2008-06-01 00:00:00
abstract::The sequence of events in the functional body pattern formation during the somatic embryo development in cowpea suspensions is described under three heads. Early stages of somatic embryogenesis were characterized by both periclinal and anti-clinal cell divisions. Differentiation of the protoderm cell layer by periclin...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0966-4
更新日期:2005-11-01 00:00:00
abstract::Heat shock transcription factors (Hsfs) are the terminal components of the signal transduction chain mediating the activation of genes responsive to both heat stress and a large number of chemical stressors. This paper aims to clone Hsf from lily and characterize its function by analyses of mRNA expression, transactiv...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0873-1
更新日期:2010-08-01 00:00:00
abstract:KEY MESSAGE:MANNANASE7 gene in Brassica napus L. encodes a hemicellulose which located at cell wall or extracellular space and dehiscence-resistance can be manipulated by altering the expression of MANNANASE7. Silique dehiscence is an important physiological process in plant reproductive development, but causes heavy y...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02638-5
更新日期:2021-02-01 00:00:00
abstract::Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234368
更新日期:1992-07-01 00:00:00
abstract::Recently, five novel fluorescent proteins have been isolated from non-bioluminescent species of reef-coral organisms and have been made available through ClonTech. They are AmCyan, AsRed, DsRed, ZsGreen and ZsYellow. These proteins are valuable as reporters for transformation because they do not require a substrate or...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0690-x
更新日期:2003-11-01 00:00:00
abstract::Plating rice anthers on a semisolid induction medium containing 250 or 500 mg/l colchicine for 24 or 48 h-incubations followed by transfer to colchicine-free medium and standard anther culture procedures resulted in overall 1.5- to 2.5- fold increases in doubled haploid green plant productions compared to control anth...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231961
更新日期:1994-05-01 00:00:00
abstract:KEY MESSAGE:Effective microspore embryogenesis in triticale is determined by a specific hormonal homeostasis: low value of IAA/cytokinins, IAA/ABA and cytokinins/ABA ratios as well as proper endogenous/exogenous auxin balance, which favours androgenic structure formation and green plant regeneration ability. The concen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1686-4
更新日期:2015-01-01 00:00:00
abstract:: A novel procedure has been developed to produce rice (Oryza sativa L.) tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. First, sublethal and lethal concentrations of PPT on 7-day-old seedlings were determined and morphogenetic events in response to the PPT treatment evaluated. Differen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000276
更新日期:2000-12-01 00:00:00
abstract::Human growth hormone (hGH), a pituitary-derived polypeptide, evidences a wide range of biological functions, including protein synthesis, cell proliferation, and metabolism. A synthetic hGH gene (shGH) has been synthesized on the basis of plant-optimized codon usage via an overlap PCR strategy and located in a plant e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0514-0
更新日期:2008-05-01 00:00:00
abstract::Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231832
更新日期:1992-02-01 00:00:00
abstract::The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenou...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0046-9
更新日期:2006-03-01 00:00:00
abstract::KEY MESSAGE : Rooting of Artemisia annua increases trichome size on leaves and helps drive the final steps of the biosynthesis of the sesquiterpene antimalarial drug, artemisinin. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1355-4
更新日期:2013-02-01 00:00:00
abstract:KEY MESSAGE:Cadmium sensitivity in sultr1;1 - sultr1;2 double mutant with limiting sulfate supply is attributed to the decreased glutathione content that affected oxidative defense but not phytochelatins' synthesis. In plants, glutathione (GSH) homeostasis plays pivotal role in cadmium (Cd) detoxification. GSH is synth...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1892-8
更新日期:2016-02-01 00:00:00
abstract::Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000198
更新日期:2000-09-01 00:00:00
abstract:KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2184-2
更新日期:2017-11-01 00:00:00
abstract::The anthracnose fungus Colletotrichum gloeosporioides deleteriously affects unripe pepper fruit, but not ripe fruit. Here, we show that the induction of local acquired resistance (LAR) by salicylic acid (SA), 2,6-dichloroisonicotinic acid, or benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester pretreatment pro...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0756-5
更新日期:2009-10-01 00:00:00
abstract::Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. In plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and increased number of leaves during the vegetative period. However, the roles of Med8 in other flowering plants are still un...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1118-7
更新日期:2011-11-01 00:00:00
abstract::We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233813
更新日期:1991-11-01 00:00:00
abstract::A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 μM 2,4-D or NAA in combination with 10 μM BA, or 25 μM 2,4-D alone. The callus grew most vigorously o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232946
更新日期:1994-06-01 00:00:00
abstract::Fertile transgenic barley (Hordeum vulgare L.) plants were obtained by high velocity particle bombardment. The plasmid pBCl was used to deliver the selectable hph gene and reporter Gus gene into immature embryo. After the selection culture 18 hygromycin resistant plants were obtained. Samples for Southern hybridizatio...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232038
更新日期:1995-02-01 00:00:00
abstract::Cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. were found to produce a large amount of berberine (400-800 mg/l) when 5-10 μM 6-benzyladenine was added to Linsmaier and Skoog's medium containing 100 μM 1-naphthaleneacetic acid. Most of the berberine produced was released continuously from the cell...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269306
更新日期:1984-12-01 00:00:00
abstract::Gastrodia elata Bl. is an achlorophyllous orchid plant feeding on the fungus Armillaria mellea. The plant lives underground during its life cycle except for florescence. Gastrodianins, members of the superfamily of monocot mannose-binding protein (MBP), have been identified from Gastrodia elata, yet their physiologica...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0226-2
更新日期:2007-03-01 00:00:00
abstract::Ethylene emanation rates were assessed from leaf tissues of an embryogenic seed plant (Cycle 0) and regeneration cycle plants selected for enhanced embryogenesis (Cycles I, II and IV). In all experiments, ethylene was assessed from the basal 1 cm portion of the innermost leaf. Ethylene emanation was five-fold higher i...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272059
更新日期:1989-03-01 00:00:00
abstract:: Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from immature zygotic embryos of Acacia mangium. Embryogenic callus was induced on MS medium containing combinations of TDZ (1-2 mg/l), IAA (0.25-2 mg/l) and a mixture of amino acids. Globular embryos developed on embryogenic ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000288
更新日期:2001-01-01 00:00:00
abstract:: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050753
更新日期:2000-04-01 00:00:00
abstract::The activity and activation potential of polyphenoloxidase (PPO, E.C. 1.10.3.1.) of tissue from shoot tips, adult leaves and embryogenic and non-embryogenic cell suspension cultures of Euphorbia pulcherrima was investigated using an oxygen probe technique. PPO derived from differentiated in vivo plant tissue (shoot ti...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234048
更新日期:1995-04-01 00:00:00