Abstract:
: Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from immature zygotic embryos of Acacia mangium. Embryogenic callus was induced on MS medium containing combinations of TDZ (1-2 mg/l), IAA (0.25-2 mg/l) and a mixture of amino acids. Globular embryos developed on embryogenic callus cultured on the induction medium. Nearly 42% of embryogenic cultures with globular embryos produced torpedo- and cotyledonary-stage embryos by a two-step maturation phase. The first stage occurred on 1/2-strength MS basal medium containing 30 g/l sucrose and 5 mg/l GA3 followed by the second stage on 1/2-strength MS basal medium containing 50 g/l sucrose. Of the cotyledonary-stage somatic embryos, 11% germinated into seedlings that could be successfully transferred to pots. Light- and scanning electron microscopy showed that the somatic embryos originated from single cells of the embryogenic callus. Further, a single cell layer could be detected beneath the developing somatic embryos that appeared to be a demarcation layer isolating the somatic proembryonic structure from the rest of the maternal callus. A suspensor-like structure connected the globular embryos to the demarcation layer. This is the first successful report of plant regeneration through somatic embryogenesis for this economically important tropical forest species.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Xie DY,Hong Ydoi
10.1007/s002990000288subject
Has Abstractpub_date
2001-01-01 00:00:00pages
34-40issue
1eissn
0721-7714issn
1432-203Xpii
10.1007/s002990000288journal_volume
20pub_type
杂志文章abstract::We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...
journal_title:Plant cell reports
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pub_type: 杂志文章
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pub_type: 社论
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章,评审
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更新日期:2001-07-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1987-04-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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