Isolation and functional characterization of a novel rice constitutive promoter.

Abstract:

KEY MESSAGE:A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineering. In this report, we isolated a novel promoter, Oscon1 promoter (P OsCon1 ), from the 5' upstream region of a constitutively expressed rice gene OsDHAR1. In P OsCon1 ::GUS transgenic rice, we showed that P OsCon1 had a broad expression spectrum in all tested tissues. The expression of the promoter was further analyzed in comparison with the previously characterized strong constitutive promoters. P OsCon1 exhibited comparable activity to OsCc1, OsAct1 or ZmUbi promoters in most tissues, and more active than 35S promoter in roots, seeds, and calli. Further quantitative assays indicated that P OsCon1 activity was not affected by developmental stages or by environmental factors. Further, 5'-deletions analysis indicated that the distinct regions might contribute to the strong expression of P OsCon1 in different tissues. Overall, our results suggest that P OsCon1 is a novel constitutive promoter, which could potentially use in transgenic crop development.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Li J,Xu RF,Qin RY,Ma H,Li H,Zhang YP,Li L,Wei PC,Yang JB

doi

10.1007/s00299-014-1644-1

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

1651-60

issue

10

eissn

0721-7714

issn

1432-203X

journal_volume

33

pub_type

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