Long-term optimized embryogenic cultures in durum wheat (Triticum durum Desf.).

Abstract:

:Five varieties of durum wheat: Appulo, Ofanto, Latino, Creso, and Castello (Triticum durum Desf.) adapted to the semi-arid mediterranean environment have been tested for their in vitro response. Compact, embryogenic, highly regenerable calli originated from primary callus derived through proliferation of the scutellum of immature embryos explanted in the presence of 2,4 dichlorophenoxyacetic acid. Selective subculture of the white, compact, embryogenic sectors led to the establishment of long-term cultures. Regeneration occurred on hormone-free medium either via germination of somatic embryos, or via multiple-shoot formation probably due to precocious germination of somatic embryos. The three varieties, Ofanto, Creso and Appulo, were the best responding genotypes. Callus fragmentation and two subsequent transfers onto fresh medium at 7-day intervals yielded a frequency of plant regeneration of some 25-40 plantlets per gram fresh weight callus in 21 days on Murashige and Skoog's hormone-free medium. Plantlets could be efficiently established in soil, thus confirming the possibility of biotechnological approaches with varieties of this crop species.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Borrelli GM,Lupotto E,Locatelli F,Wittmer G

doi

10.1007/BF00193145

subject

Has Abstract

pub_date

1991-09-01 00:00:00

pages

296-9

issue

6-7

eissn

0721-7714

issn

1432-203X

journal_volume

10

pub_type

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