Will the EU stay out of step with science and the rest of the world on plant breeding innovation?

abstract：: An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. T...

journal_title：Plant cell reports

authors： Eady CC,Weld RJ,Lister CE

更新日期：2000-03-01 00:00:00

abstract：:Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...

journal_title：Plant cell reports

authors： Moore PJ,Moore AJ,Collins GB

更新日期：1994-07-01 00:00:00

abstract：:An improved protocol forAgrobacterium-mediated transformation of the tomato cultivar Moneymaker was developed by examining the effects of six different factors on the efficiency of transformation. Explant size, explant orientation, gelling agent and plate sealant were found to affect transformation efficiency. Two oth...

journal_title：Plant cell reports

authors： Frary A,Earle ED

更新日期：1996-12-01 00:00:00

abstract：:Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspo...

journal_title：Plant cell reports

authors： Burnett L,Yarrow S,Huang B

更新日期：1992-05-01 00:00:00

abstract：:A differential expression study on the Saccharum spp.-Sporisorium scitamineum pathogenic interaction was carried out in the susceptible Ja60-5 and the resistant M31/45 genotypes. Using cDNA-AFLP analysis, a total of 64 transcript-derived fragments (TDFs) was found to be differentially expressed, with the majority (67....

journal_title：Plant cell reports

authors： Lao M,Arencibia AD,Carmona ER,Acevedo R,Rodríguez E,León O,Santana I

更新日期：2008-06-01 00:00:00

abstract：KEY MESSAGE:Sensitivity to Erysiphe in Noccaea praecox with low metal supply is related to the failure in enhancing SA. Cadmium protects against fungal-infection by direct toxicity and/or enhanced fungal-induced JA signaling. Metal-based defense against biotic stress is an attractive hypothesis on evolutionary advantag...

journal_title：Plant cell reports

authors： Llugany M,Martin SR,Barceló J,Poschenrieder C

更新日期：2013-08-01 00:00:00

abstract：:A procedure has been developed for the clonal propagation of Picrorhiza kurroa Royle ex Benth. through shoot tip culture. Murashige and Skoog's medium (1962) supplemented with kinetin (3.0 to 5.0 mg/l) supported rapid proliferation of multiple shoots from the explants. Addition of indole-3-acetic acid (1.0 mg/l) to th...

journal_title：Plant cell reports

authors： Lal N,Ahuja PS,Kukreja AK,Pandey B

更新日期：1988-05-01 00:00:00

abstract：:Recently, five novel fluorescent proteins have been isolated from non-bioluminescent species of reef-coral organisms and have been made available through ClonTech. They are AmCyan, AsRed, DsRed, ZsGreen and ZsYellow. These proteins are valuable as reporters for transformation because they do not require a substrate or...

journal_title：Plant cell reports

authors： Wenck A,Pugieux C,Turner M,Dunn M,Stacy C,Tiozzo A,Dunder E,van Grinsven E,Khan R,Sigareva M,Wang WC,Reed J,Drayton P,Oliver D,Trafford H,Legris G,Rushton H,Tayab S,Launis K,Chang YF,Chen DF,Melchers L

更新日期：2003-11-01 00:00:00

abstract：:Self-incompatibility (SI) has been studied extensively at the molecular level in Solanaceae, Rosaceae and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility (GSI). In the present study, four PpsS-haplotypes (Prunus pseudocerasus S-haplotypes) comprising at least two genes, i.e., PpsS-RNase (P. ps...

journal_title：Plant cell reports

authors： Huang SX,Wu HQ,Li YR,Wu J,Zhang SJ,Heng W,Zhang SL

更新日期：2008-06-01 00:00:00

abstract：:Sugarcane cell suspensions were initiated from leaf callus and sub-cultured every 7 to 10 days by alternate transfer to MS based medium with 3.0 and 1.0 mg 1(-1)2,4-D. Suspensions older than 3 months gave the most reproducible yields of protoplasts. Isolated protoplasts required 50 mM Ca(2+) in the washing solution an...

journal_title：Plant cell reports

authors： Chen WH,Davey MR,Power JB,Cocking EC

更新日期：1988-08-01 00:00:00

abstract：:In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in cu...

journal_title：Plant cell reports

authors： Sharma VK,Jethwani V,Kothari SL

更新日期：1993-08-01 00:00:00

abstract：KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...

journal_title：Plant cell reports

authors： Machado RD,Christoff AP,Loss-Morais G,Margis-Pinheiro M,Margis R,Körbes AP

更新日期：2015-07-01 00:00:00

abstract：:The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...

journal_title：Plant cell reports

authors： Sethi U,Basu A,Mukherjee SG

更新日期：1990-03-01 00:00:00

abstract：:A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 μM 2,4-D or NAA in combination with 10 μM BA, or 25 μM 2,4-D alone. The callus grew most vigorously o...

journal_title：Plant cell reports

authors： Barna KS,Wakhlu AK

更新日期：1994-06-01 00:00:00

abstract：: Embryogenic soybean [Glycine max (L.) Merrill.] suspension cultures were bombarded with five different gene constructions encoding the jellyfish (Aequorea victoria) green fluorescent protein (GFP). These constructions had altered codon usage compared to the native GFP gene and mutations that increased the solubility ...

journal_title：Plant cell reports

authors： Ponappa T,Brzozowski AE,Finer JJ

更新日期：1999-11-01 00:00:00

abstract：KEY MESSAGE:Cowpea miRNAs and Argonaute genes showed differential expression patterns in response to CPSMV challenge Several biotic stresses affect cowpea production and yield. CPSMV stands out for causing severe negative impacts on cowpea. Plants have two main induced immune systems. In the basal system (PTI, PAMP-tri...

journal_title：Plant cell reports

authors： Martins TF,Souza PFN,Alves MS,Silva FDA,Arantes MR,Vasconcelos IM,Oliveira JTA

更新日期：2020-08-01 00:00:00

abstract：:Cotyledon explants from zygotic embryos of Panax ginseng produced somatic embryos on Murashige and Skoog basal medium without growth regulators. Somatic embryos developed directly from epidermal cells at the cotyledon base. Somatic embryos were always formed from the side of the cotyledon opposite to the one attached ...

journal_title：Plant cell reports

authors： Choi YE,Yang DC,Kim HS,Choi KT

更新日期：1997-10-01 00:00:00

abstract：:Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...

journal_title：Plant cell reports

authors： Lin CS,Chen CT,Lin CC,Chang WC

更新日期：2003-06-01 00:00:00

abstract：:While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was...

journal_title：Plant cell reports

authors： Sorkina A,Bardosh G,Liu YZ,Fridman I,Schlizerman L,Zur N,Or E,Goldschmidt EE,Blumwald E,Sadka A

更新日期：2011-09-01 00:00:00

abstract：:The article 'Instability of endosperm development in amphiploids and their parental species in the genus Avena L.', written by Paulina Tomaszewska and Romuald Kosina, was originally published electronically on the publisher's internet portal on 22 May with an incorrect copyright line. As the article was due to be publ...

journal_title：Plant cell reports

authors： Tomaszewska P,Kosina R

更新日期：2019-01-01 00:00:00

abstract：:The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...

journal_title：Plant cell reports

authors： Schiel O,Jarchow-Redecker K,Piehl GW,Lehmann J,Berlin J

更新日期：1984-02-01 00:00:00

abstract：:A procedure for inducing somatic embryos in shoot apex explants (2 mm) excised from shoot proliferation cultures established from adult oak trees (Quercus robur) was investigated. Embryogenesis was induced in shoot tip as well as leaf explants in three out of the five genotypes evaluated. Somatic embryos were formed b...

journal_title：Plant cell reports

authors： San-José MC,Corredoira E,Martínez MT,Vidal N,Valladares S,Mallón R,Vieitez AM

更新日期：2010-06-01 00:00:00

abstract：:Sugarcane white leaf (SCWL)-diseased sugarcane plants collected from Udornthani Province, in north-eastern Thailand, were the source for tissue culture experiments. Explants from axillary buds, meristem tips, and leaves grew optimally in Murashige-Skoog medium containing 0.5 mg/l alpha-naphthaleneacetic acid, 0.5 mg/l...

journal_title：Plant cell reports

authors： Wongkaew P,Fletcher J

更新日期：2004-11-01 00:00:00

abstract：:Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Fo...

journal_title：Plant cell reports

authors： Patat-Ochatt EM,Boccon-Gibod J,Duron M,Ochatt SJ

更新日期：1993-01-01 00:00:00

abstract：:Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...

journal_title：Plant cell reports

authors： Datta SK,Datta K,Potrykus I

更新日期：1990-09-01 00:00:00

abstract：:Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...

journal_title：Plant cell reports

authors： El Quakfaoui S,Potvin C,Brzezinski R,Asselin A

更新日期：1995-12-01 00:00:00

abstract：:Developmental biology studies in general benefit from model organisms that are well characterized. Arabidopsis thaliana fulfills this criterion and represents one of the best experimental systems to study developmental processes in higher plants. Light is a crucial factor that drives photosynthesis, but that also regu...

journal_title：Plant cell reports

authors： Boron AK,Vissenberg K

更新日期：2014-05-01 00:00:00

abstract：KEY MESSAGE:The Arabidopsis SAUR36, renamed RAG1, integrates auxin and gibberellin signals to regulate apical hook maintenance in etiolated seedlings, hypocotyl elongation in the light and fertility. Phytohormone signalling intermediates integrate responses to developmental cues and the variety of environmental inputs ...

journal_title：Plant cell reports

authors： Stamm P,Kumar PP

更新日期：2013-06-01 00:00:00

abstract：:This article describes conditions to optimize the yield of viable protoplasts from callus tissue of Asparagus densiflorus cv. Sprengeri and their subsequent regeneration into plantlets. Callus tissue was initiated by culturing spear sections (5-7 mm) on Murashige and Skoog (MS) medium supplemented with 0.8% (wt/vol) B...

journal_title：Plant cell reports

authors： Benmoussa M,Mukhopadhyay S,Desjardins Y

更新日期：1997-12-01 00:00:00

abstract：:Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,...

journal_title：Plant cell reports

authors： Rout GR,Das P

更新日期：1994-09-01 00:00:00