Abstract:
:This article describes conditions to optimize the yield of viable protoplasts from callus tissue of Asparagus densiflorus cv. Sprengeri and their subsequent regeneration into plantlets. Callus tissue was initiated by culturing spear sections (5-7 mm) on Murashige and Skoog (MS) medium supplemented with 0.8% (wt/vol) Bacto agar, 3% (wt/vol) sucrose, 0.5 mg/l each of nicotinic acid, pyridoxine-HCl, and thiamine-HCl, 1 mg/l p-chlorophenoxyaceticacid (pCPA) and 1 mg/l 6-benzylaminopurine (BAP). The maximum protoplast yield was obtained in a mixture of 1% (wt/vol) Cellulysin, 0.8% (wt/vol) Rhozyme HP 150 and 0.3% (wt/vol) Macerase, dissolved in cell protoplast wash salt solution with 7 mM CaCl2.2H2O, 3 mM MES, 0.6 M glucose, and 0.1 M mannitol. First divisions were observed after 3-4 days of initial culture. The plating efficiency was highest (7.8%) in half-strength MS semisolid medium containing 1 g/l glutamine, 0.6 M glucose, 0.1 M mannitol, 0.5 mg/l folic acid, 0.05 mg/l biotin, 2 mg/l ascorbic acid, 1 mg/l α-naphthaleneacetic acid, 0.5 mg/l zeatin, and 0.1% (wt/vol) Gelrite. Protoplast-derived microcolonies and microcalli were cultured on the same medium on which the primary callus culture was initiated. After 10-12 weeks, calli were transferred to shoot regeneration medium containing MS salts, 1 mg/l BAP, 0.5 mg/l pCPA and 0.2% Gelrite. Shoots (3-4 cm) were then transferred to MS rooting medium with 2 mg/l indole-3-butyric acid, and 0.2% Gelrite. Plantlets were obtained within 4-5 weeks.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Benmoussa M,Mukhopadhyay S,Desjardins Ydoi
10.1007/s002990050364subject
Has Abstractpub_date
1997-12-01 00:00:00pages
123-128issue
2eissn
0721-7714issn
1432-203Xpii
10.1007/s002990050364journal_volume
17pub_type
杂志文章abstract::Suspension cultured cells of the moss, Barbula unguiculata, grow actively in both light and dark culture. Light-grown cells contain chlorophyll and exhibit an undifferentiated callus form. When cells are transferred to a dark condition, they develop into protonemata. Protonemata formation in the dark can be inhibited ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272039
更新日期:1989-03-01 00:00:00
abstract::Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239891
更新日期:1994-01-01 00:00:00
abstract::We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants wa...
journal_title:Plant cell reports
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更新日期:1998-03-01 00:00:00
abstract::The growth and sweet diterpene glucosides of Stevia plants propagated by stem-tip cultures were compared with those of the control plants propagated by seeds. There was no significant difference between the two groups both in growth and in chemical composition. As for the contents of sweet diterpene glucosides, howeve...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270194
更新日期:1984-10-01 00:00:00
abstract::Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements us...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-003-0616-7
更新日期:2003-06-01 00:00:00
abstract::Protoplasts isolated from cultured rice cells of an A-58 cytoplasmic male sterile line (A-58 MS)(Oryza sativa L.) were used to investigate the regeneration of rice plants. A cultured cell line (T3) of A-58 MS with a high growth rate and dense cytoplasm was selected. About 10% of the protoplasts prepared from this esta...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269240
更新日期:1986-04-01 00:00:00
abstract::The voltage-dependent anion channels (VDACs), mitochondrial outer membrane components, are present in organisms from fungi to animals and plants. They are thought to function in the regulation of metabolite transport between mitochondria and the cytoplasm. Sufficient knowledge on plant VDACs has been accumulated, so t...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-009-0741-z
更新日期:2009-09-01 00:00:00
abstract:KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2184-2
更新日期:2017-11-01 00:00:00
abstract::In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050459
更新日期:1998-05-01 00:00:00
abstract:: St. John's wort (Hypericum perforatum. cv 'Anthos') is a medicinal plant with evidence of efficacy as an anti-depressant. The present report describes the development of an in vitro regeneration system that utilizes thidiazuron [N-phenyl-N'-(1,2,3-thidiazol-yl)urea] for the induction of de novo shoots on etiolated hy...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050776
更新日期:2000-05-01 00:00:00
abstract::Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0695-1
更新日期:2009-06-01 00:00:00
abstract::This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0638-2
更新日期:2009-03-01 00:00:00
abstract:KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02571-7
更新日期:2020-11-01 00:00:00
abstract::Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitab...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01092764
更新日期:1997-04-01 00:00:00
abstract::We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233813
更新日期:1991-11-01 00:00:00
abstract::Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibite...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232042
更新日期:1991-06-01 00:00:00
abstract::A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0276-5
更新日期:2007-05-01 00:00:00
abstract::A DNA fragment encoding a 12-amino acid (aa) HIV-1 Tat transduction peptide fused to a 90-aa murine rotavirus NSP4 enterotoxin protein (Tat-NSP4(90)) was transferred to Solanum tuberosum by Agrobacterium tumefaciens-mediated transformation. The fusion gene was detected in the genomic DNA of transformed plant leaf tiss...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0697-3
更新日期:2004-01-01 00:00:00
abstract::The interconversion between 5 to 8-membered cycloalkanones and the corresponding cycloalkanols by a cell suspension culture of Nicotiana tabacum was studied. It was found that the interconversion is at equilibrium and the balance in the equilibrium depends on the number of carbon atoms in the carbocyclic ring of the s...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270167
更新日期:1983-04-01 00:00:00
abstract:: Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from immature zygotic embryos of Acacia mangium. Embryogenic callus was induced on MS medium containing combinations of TDZ (1-2 mg/l), IAA (0.25-2 mg/l) and a mixture of amino acids. Globular embryos developed on embryogenic ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000288
更新日期:2001-01-01 00:00:00
abstract::Oil palm is a major economic crop for Malaysia. The major challenges faced by the industry are labor shortage, availability of arable land and unstable commodity price. This has caused the industry to diversify its applications into higher value products besides increasing its yield. While conventional breeding has it...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-014-1722-4
更新日期:2015-04-01 00:00:00
abstract:KEY MESSAGE:The radish WRKY gene family was genome-widely identified and played critical roles in response to multiple abiotic stresses. The WRKY is among the largest transcription factors (TFs) associated with multiple biological activities for plant survival, including control response mechanisms against abiotic stre...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2190-4
更新日期:2017-11-01 00:00:00
abstract::Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234510
更新日期:1994-07-01 00:00:00
abstract::The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1124-9
更新日期:2011-12-01 00:00:00
abstract::Somatic hybrids between Solanum tuberosum cv 'Brodick' and S. sanctae-rosae were selected for having elevated chromosome numbers, intermediate morphology and potato cyst nematode resistance (derived from S. sanctae-rosae). DNA was extracted from this material and subjected to inter-SSR PCR directed by oligonucleotide ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050735
更新日期:1999-12-01 00:00:00
abstract::Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protopla...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269287
更新日期:1985-08-01 00:00:00
abstract::Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231832
更新日期:1992-02-01 00:00:00
abstract::Rice protoplasts (Oryza sativa L. v Taipei 309) have been transformed to kanamycin resistance following uptake of pCaMVNEO induced by electroporation, PEG and PEG combined with electroporation. Protoplast-derived colonies selected on medium containing 100 μg/ml of kanamycin expressed NPTII activity, and contained DNA ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269528
更新日期:1988-10-01 00:00:00
abstract:: An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotrans...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050004
更新日期:2000-01-01 00:00:00
abstract::A microscopical study was performed in order to localize chromogenic valepotriates in roots (in vivo) and in callus and root organ cultures (in vitro) of valerian plants. These in vitro cultures (producing valepotriates) contained lipid droplets having the same aspect as those containing essential oil described in the...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270186
更新日期:1983-12-01 00:00:00