Rapid and efficient transformation of diploid Medicago truncatula and Medicago sativa ssp. falcata lines improved in somatic embryogenesis.

Abstract:

:We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants was further improved by three successive in vitro regeneration cycles resulting in the line R108-1(c3). Agrobacterium tumefaciens-mediated transformation of leaf explants was carried out with promoter-gus constructs of two early nodulins (MsEnod12A and MsEnod12B) and one late nodulin (Srglb3). The transgenic plants thus produced on all explants within 3-4 months remained diploid and were fertile. This protocol appears to be the most efficient and fastest reported so far for leguminous plants.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Trinh TH,Ratet P,Kondorosi E,Durand P,Kamaté K,Bauer P,Kondorosi A

doi

10.1007/s002990050405

subject

Has Abstract

pub_date

1998-03-01 00:00:00

pages

345-355

issue

5

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990050405

journal_volume

17

pub_type

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