Abstract：
:Transgenic papaya (Carica papaya L.) plants were regenerated from embryogenic cultures that were cocultivated with a disarmed C58 strain of Agrobacterium tumefaciens containing one of the following binary cosmid vectors: pGA482GG or pGA482GG/cpPRV-4. The T-DNA region of both binary vectors includes the chimeric genes for neomycin phosphotransferase II (NPTII) and ß-glucuronidase (GUS). In addition, the plant expressible coat protein (cp) gene of papaya ringspot virus (PRV) is flanked by the NPTII and GUS genes in pGA482GG/cpPRV-4. Putative transformed embryogenic papaya tissues were obtained by selection on 150 μg·ml(-1) kanamycin. Four putative transgenic plant lines were obtained from the cp gene(-) vector and two from the cp gene(+) vector. GUS and NPTII expression were detected in leaves of all putative transformed plants tested, while PRV coat protein expression was detected in leaves of the PRV cp gene(+) plant. The transformed status of these papaya plants was analyzed using both polymerase chain reaction amplification and genomic blot hybridization of the NPTII and PRV cp genes. Integration of these genes into the papaya genome was demonstrated by genomic blot hybridizations. Thus, like numerous other dicotyledonous plant species, papayas can be transformed with A. tumefaciens and regenerated into phenotypically normal-appearing plants that express foreign genes.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Fitch MM,Manshardt RM,Gonsalves D,Slightom JLdoi
10.1007/BF00237128subject
Has Abstractpub_date
1993-03-01 00:00:00pages
245-9issue
5eissn
0721-7714issn
1432-203Xjournal_volume
12pub_type
杂志文章abstract：:A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231799
更新日期：1991-03-01 00:00:00
abstract：KEY MESSAGE:Phosphate starvation increased the production of phenolic acids by inducing the key enzyme genes in a positive feedback pathway in Saliva miltiorrhiza hairy roots. SPX may be involved in this process. Salvia miltiorrhiza is a wildly popular traditional Chinese medicine used for the treatment of coronary hea...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2007-x
更新日期：2016-09-01 00:00:00
abstract：:Hypocotyl explants of 1 and 10 mm lengths were excised from 12-day-old in vitro-grown seedlings of Albizia richardiana. The larger pieces, after 40 days of culture, developed shoots along with green calli on B5 + BAP (10(-7)-10(-5)M), while the smaller segments produced only green calli on B5+BAP (10(-7)-10(-4)M) medi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272982
更新日期：1988-01-01 00:00:00
abstract：:Seeds are heterogeneous storage reserves with wide array of storage compounds that include various soluble carbohydrates, starch polymer, storage proteins and lipids. These stored reserves comprise 70% of the world's caloric intake in the form of food and animal feed produced through sustainable agriculture, which con...
journal_title：Plant cell reports
pub_type： 社论
doi：10.1007/s00299-017-2135-y
更新日期：2017-05-01 00:00:00
abstract：:Six sugarbeet (Beta vulgaris L.) lines (GWI-248, SPB-11, MonoHy 55, SMS-1, EL45 and FC607) were tested for regeneration. Shoot cultures were initiated in vitro from naked, sterilized embryos obtained from mature seed. Excised petioles from cultured shoots were plated on Gamborg's B5 medium and four modified Murashige ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272972
更新日期：1988-01-01 00:00:00
abstract：:Five varieties of durum wheat: Appulo, Ofanto, Latino, Creso, and Castello (Triticum durum Desf.) adapted to the semi-arid mediterranean environment have been tested for their in vitro response. Compact, embryogenic, highly regenerable calli originated from primary callus derived through proliferation of the scutellum...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00193145
更新日期：1991-09-01 00:00:00
abstract：KEY MESSAGE:Cowpea miRNAs and Argonaute genes showed differential expression patterns in response to CPSMV challenge Several biotic stresses affect cowpea production and yield. CPSMV stands out for causing severe negative impacts on cowpea. Plants have two main induced immune systems. In the basal system (PTI, PAMP-tri...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02548-6
更新日期：2020-08-01 00:00:00
abstract：:The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01088278
更新日期：1997-02-01 00:00:00
abstract：:In this study, we report the obtaining of carrot plants expressing human interferon alpha-2b via Agrobacterium-mediated transformation using two vector constructs containing the sequence coding for interferon gene fused with Nicotiana plumbagenifolia calreticulin apoplast targeting signal driven by 35S CaMV promoter a...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0942-5
更新日期：2011-03-01 00:00:00
abstract：:A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232265
更新日期：1990-12-01 00:00:00
abstract：KEY MESSAGE:Overexpression of Withania somnifera SGT gene (WssgtL3.1) in transgenic Arabidopsis improves various agronomic and physiological traits and alters conjugated sterol levels to mitigate the effect of salt stress. Sterols are essential constituents of cell membranes that are involved in several biological func...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-021-02666-9
更新日期：2021-02-01 00:00:00
abstract：KEY MESSAGE:Prolamin-GFP fusion proteins, expressed under the control of native prolamin promoters, were localized in specific layers of PB-Is. Prolamin-GFP fusion proteins were gradually digested from outside by pepsin digestion. In rice seed endosperm, protein body type I (PB-I) has a layered structure consisting of ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1960-8
更新日期：2016-06-01 00:00:00
abstract：:This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0638-2
更新日期：2009-03-01 00:00:00
abstract：:Suspension cultures derived from Lycium chinense Miller seedlings produced significant amounts of a hepatoprotective cerebroside. Callus was induced from the stem of aseptic seedlings of L. chinense and maintained on MS solid media supplemented with 1.0 ppm 2,4-D and 0.1 ppm kinetin. Suspension cultures were establish...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050566
更新日期：1998-12-01 00:00:00
abstract：:Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00239891
更新日期：1994-01-01 00:00:00
abstract：: St. John's wort (Hypericum perforatum. cv 'Anthos') is a medicinal plant with evidence of efficacy as an anti-depressant. The present report describes the development of an in vitro regeneration system that utilizes thidiazuron [N-phenyl-N'-(1,2,3-thidiazol-yl)urea] for the induction of de novo shoots on etiolated hy...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050776
更新日期：2000-05-01 00:00:00
abstract：:In the present paper, attempts were made to explore the possibility of employing ultraviolet (UV) irradiation in citrus asymmetric fusion for transfer of limited amount of favorable traits from a desirable cultivar to a target one. Exposure of Satsuma mandarin (Citrus unshiu Marc.) embryogenic protoplasts to UV at an ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0350-7
更新日期：2007-08-01 00:00:00
abstract：:Optimum conditions for the isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato (Ipomoea batatas) were investigated. Growth phase of callus and the ratio of callus-enzyme solution affected the yield of protoplasts. Composition of the medium and protoplast density were examined fo...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269369
更新日期：1985-10-01 00:00:00
abstract：KEY MESSAGE:Induced mutations in the waxy locus in rice endosperm did not abolish GBSS activity completely. Compensatory mechanisms in endosperm and leaves caused a major reprogramming of the starch biosynthetic machinery. The mutation of genes in the starch biosynthesis pathway has a profound effect on starch quality ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02388-z
更新日期：2019-03-01 00:00:00
abstract：:The AtGRP5 gene from Arabidopsis thaliana encodes a glycine-rich protein which has a major activity in protoderm-derived cells and is expressed in cells that undergo the first anatomical modifications leading to somatic embryo development. It has been previously demonstrated that its minimum promoter is 316 bp long in...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0835-7
更新日期：2010-05-01 00:00:00
abstract：:The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0920-5
更新日期：2005-03-01 00:00:00
abstract：:The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270221
更新日期：1984-02-01 00:00:00
abstract：:The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0917-0
更新日期：2005-06-01 00:00:00
abstract：: Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the us...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050014
更新日期：2000-01-01 00:00:00
abstract：:Gastrodia elata Bl. is an achlorophyllous orchid plant feeding on the fungus Armillaria mellea. The plant lives underground during its life cycle except for florescence. Gastrodianins, members of the superfamily of monocot mannose-binding protein (MBP), have been identified from Gastrodia elata, yet their physiologica...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0226-2
更新日期：2007-03-01 00:00:00
abstract：:Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditions favorable to dir...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232170
更新日期：1992-04-01 00:00:00
abstract：:Protoplasts isolated from cell suspensions of albinoMedicago borealis andM. sativa were fused chemically, using two methods, and electrically. Although a small scale method of chemical fusion gave the highest fusion frequency, electrofusion was the superior technique on the basis of throughput of green somatic hybrid ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00735772
更新日期：1989-05-01 00:00:00
abstract：:The lateral cotyledonary meristems of germinatingPisum sativum cv. Puget seeds were used to develop a reproducibleAgrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerab...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01142315
更新日期：1997-05-01 00:00:00
abstract：KEY MESSAGE:The recovery of non-functional-enhanced green fluorescence protein can be used as indicator to facilitate the identification of mutants generated by CRISPR/Cas9. The CRISPR/Cas9 system is a powerful tool for genome editing and it has been employed to knock out genes of interest in multiple plant species. Id...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02465-3
更新日期：2019-12-01 00:00:00
abstract：:Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233055
更新日期：1993-08-01 00:00:00
