High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants.

abstract：:A novel member of the WRKY gene family, designated TcWRKY53, was isolated from a cadmium (Cd)-treated Thlaspi caerulescens cDNA library by differential screening. WRKY proteins specifically bind to W-boxes, which are found in the promoters of many genes involved in defense and response to environmental stress. TcWRKY5...

journal_title：Plant cell reports

authors： Wei W,Zhang Y,Han L,Guan Z,Chai T

更新日期：2008-04-01 00:00:00

abstract：UNLABELLED:Musa acuminata ssp. burmannica, one of the wild progenitors contributing 'A genome' to the present-day dessert bananas, has a long evolutionary history intervened by human activities. In this study, ISSR markers were used to analyze the pattern of genetic variation and differentiation in 32 individuals along...

journal_title：Plant cell reports

authors： Padmesh P,Mukunthakumar S,Vineesh PS,Skaria R,Hari Kumar K,Krishnan PN

更新日期：2012-09-01 00:00:00

abstract：:We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were ...

journal_title：Plant cell reports

authors： Renau-Morata B,Arrillaga I,Segura J

更新日期：2006-07-01 00:00:00

abstract：:Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...

journal_title：Plant cell reports

authors： He DG,Yang YM,Scott KJ

更新日期：1992-02-01 00:00:00

abstract：:A collection of 29 pathogenesis-related 10 (PR10) genes of Medicago sativa and Medicago truncatula showed that they were almost all obtained from cDNA libraries of tissues undergoing abiotic or biotic stresses. The predicted proteins could be divided into two subclasses, PR10.1 and PR10.2, but in silico predicted mode...

journal_title：Plant cell reports

authors： Bahramnejad B,Goodwin PH,Zhang J,Atnaseo C,Erickson LR

更新日期：2010-11-01 00:00:00

abstract：:Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into en...

journal_title：Plant cell reports

authors： Padhya MA,Mehta AR

更新日期：1982-12-01 00:00:00

abstract：:A double-gene construct with one chitinase and one beta-1,3-glucanase gene from barley, both driven by enhanced 35S promoters, was transformed into oilseed rape. From six primary transformants expressing both transgenes 10 doubled haploid lines were produced and studied for five generations. The number of inserted cop...

journal_title：Plant cell reports

authors： Melander M,Kamnert I,Happstadius I,Liljeroth E,Bryngelsson T

更新日期：2006-09-01 00:00:00

abstract：: A novel procedure has been developed to produce rice (Oryza sativa L.) tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. First, sublethal and lethal concentrations of PPT on 7-day-old seedlings were determined and morphogenetic events in response to the PPT treatment evaluated. Differen...

journal_title：Plant cell reports

authors： Toldi O,Tóth S,Oreifig AS,Kiss E,Jenes B

更新日期：2000-12-01 00:00:00

abstract：:We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...

journal_title：Plant cell reports

authors： Zhong H,Srinivasan C,Sticklen MB

更新日期：1991-11-01 00:00:00

abstract：KEY MESSAGE:A complete set of monosomic alien addition lines of Brassica napus with one of the seven chromosomes of Isatis indigotica and the recombinant mitochondria was developed and characterized. Monosomic alien addition lines (MAALs) are valuable for elucidating the genome structure and transferring the useful gen...

journal_title：Plant cell reports

authors： Kang L,Du X,Zhou Y,Zhu B,Ge X,Li Z

更新日期：2014-08-01 00:00:00

abstract：:An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-sup...

journal_title：Plant cell reports

authors： Watad AA,Kochba M,Nissim A,Gaba V

更新日期：1995-03-01 00:00:00

abstract：KEY MESSAGE:We developed a method for detection of mutants in a large number of plants, and found this method to be applicable to detection of a mutant allele at a concentration of 1/1000. Many techniques for SNP analysis have been developed, but most of these techniques are not so sensitive to be used for detection of...

journal_title：Plant cell reports

authors： Kohata R,Koitabashi K,Kitashiba H,Nishio T

更新日期：2018-06-01 00:00:00

abstract：:Sugarcane white leaf (SCWL)-diseased sugarcane plants collected from Udornthani Province, in north-eastern Thailand, were the source for tissue culture experiments. Explants from axillary buds, meristem tips, and leaves grew optimally in Murashige-Skoog medium containing 0.5 mg/l alpha-naphthaleneacetic acid, 0.5 mg/l...

journal_title：Plant cell reports

authors： Wongkaew P,Fletcher J

更新日期：2004-11-01 00:00:00

abstract：:The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...

journal_title：Plant cell reports

authors： Gu XF,Zhang JR

更新日期：2005-03-01 00:00:00

abstract：:We report here the successful establishment of callus, cell and root cultures from explants of in-vitro-grown plantlets of the soapwort Saponaria officinalis L. The production of saporin in the different tissue systems was evaluated by determining the capability of crude extracts to inactivate protein synthesis and by...

journal_title：Plant cell reports

authors： Di Cola A,Di Domenico C,Poma A,Spanò L

更新日期：1997-11-01 00:00:00

abstract：:Shoot buds of ginger were successfully encapsulated in 4% sodium alginate gel. Encapsulated buds were germinated in vitro to form roots and shoots. In vitro germination (emergence of sprouts) of encapsulated buds ranged from 16.7% to 81.8% on different media after 5 weeks of incubation. Normal plantlets with an averag...

journal_title：Plant cell reports

authors： Sharma TR,Singh BM,Chauhan RS

更新日期：1994-02-01 00:00:00

abstract：:Self-incompatibility (SI) has been studied extensively at the molecular level in Solanaceae, Rosaceae and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility (GSI). In the present study, four PpsS-haplotypes (Prunus pseudocerasus S-haplotypes) comprising at least two genes, i.e., PpsS-RNase (P. ps...

journal_title：Plant cell reports

authors： Huang SX,Wu HQ,Li YR,Wu J,Zhang SJ,Heng W,Zhang SL

更新日期：2008-06-01 00:00:00

abstract：:Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1-4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophen...

journal_title：Plant cell reports

authors： Fereol L,Chovelon V,Causse S,Triaire D,Arnault I,Auger J,Kahane R

更新日期：2005-08-01 00:00:00

abstract：:Optimal protoplast yields from cotyledons (2.0×10(6) protoplasts/ 0.5 g tissue) and from true leaves (5.0×10(6) protoplasts/g tissue) of two Cucumis sativus genotypes were obtained following a 16 h digestion with, respectively, 1.25% pectinase+0.5% Cellulysin and 0.5 % pectinase+ 1.0% Cellulysin. Enzyme solutions were...

journal_title：Plant cell reports

authors： Punja ZK,Tang FA,Sarmento GG

更新日期：1990-07-01 00:00:00

abstract：:The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf dis...

journal_title：Plant cell reports

authors： Passey AJ,Barrett KJ,James DJ

更新日期：2003-01-01 00:00:00

abstract：:Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been as extensively studied. We examined the effect of brassinolide on the kinetics and final division frequencies of regenerating leaf mesophyll protoplasts of Petunia hybrida Vilm v. Coman...

journal_title：Plant cell reports

authors： Oh MH,Clouse SD

更新日期：1998-09-01 00:00:00

abstract：:In an attempt to improve growth and secondary metabolite production, and to understand the possible mechanism involved in relation to the changes in physiology and activities of antioxidant enzymes, we cultured Morinda citrifolia adventitious roots in different strength (0.25, 0.50, 0.75, 1.0, 1.5 and 2.0) of Murashig...

journal_title：Plant cell reports

authors： Abdullahil Baque M,Lee EJ,Paek KY

更新日期：2010-07-01 00:00:00

abstract：:It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, ...

journal_title：Plant cell reports

authors： Manzocchi LA,Bianchi MW,Viotti A

更新日期：1989-03-01 00:00:00

abstract：:Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of call...

journal_title：Plant cell reports

authors： Castillo AM,Cistué L

更新日期：1993-01-01 00:00:00

abstract：:Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived...

journal_title：Plant cell reports

authors： Darsan Singh JK,Mat Jalaluddin NS,Sanan-Mishra N,Harikrishna JA

更新日期：2019-12-01 00:00:00

abstract：KEY MESSAGE:SnYSL3 encodes a plasma-localized transporter delivering various metal-nicotianamine complexes. The expression of SnYSL3 is up-regulated by excess Cd, suggesting an important role for SnYSL3 in response to Cd stress. The Yellow Stripe-Like (YSL) transporters have been proposed to participate in metal uptake...

journal_title：Plant cell reports

authors： Feng S,Tan J,Zhang Y,Liang S,Xiang S,Wang H,Chai T

更新日期：2017-02-01 00:00:00

abstract：:Exposure of Arabidopsis thaliana L. to an acclimation temperature (+4°C) results in a rapid increase of frost tolerance from -3°C to -7°C. This increase could be correlated to changes in soluble protein pattern. Analysis of in vitro translation products from isolated mRNA suggests that induction acts at the transcript...

journal_title：Plant cell reports

authors： Kurkela S,Franck M,Heino P,Lång V,Palva ET

更新日期：1988-12-01 00:00:00

abstract：:RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV)...

journal_title：Plant cell reports

authors： Gan D,Zhang J,Jiang H,Jiang T,Zhu S,Cheng B

更新日期：2010-11-01 00:00:00

abstract：:DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...

journal_title：Plant cell reports

authors： Nelke M,Nowak J,Wright JM,McLean NL

更新日期：1993-12-01 00:00:00

abstract：:A novel member of the AP2/ERF transcription factor family, SlERF5, was identified from a tomato mature leaf cDNA library screen. The complete DNA sequence of SlERF5 encodes a putative 244-amino acid DNA-binding protein which most likely acts as a transcriptional regulator and is a member of the ethylene responsive fac...

journal_title：Plant cell reports

authors： Pan Y,Seymour GB,Lu C,Hu Z,Chen X,Chen G

更新日期：2012-02-01 00:00:00