Sensitive mutant detection by concentrating mutant DNA with allele-specific capture and its application to analysis of contaminated grains in rice.

abstract：:In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...

journal_title：Plant cell reports

authors： Hosoyama H,Irie K,Abe K,Arai S

更新日期：1995-12-01 00:00:00

abstract：:A procedure for inducing somatic embryos in shoot apex explants (2 mm) excised from shoot proliferation cultures established from adult oak trees (Quercus robur) was investigated. Embryogenesis was induced in shoot tip as well as leaf explants in three out of the five genotypes evaluated. Somatic embryos were formed b...

journal_title：Plant cell reports

authors： San-José MC,Corredoira E,Martínez MT,Vidal N,Valladares S,Mallón R,Vieitez AM

更新日期：2010-06-01 00:00:00

abstract：:A novel strategy for the production and maintenance of morphogenic callus for 1 year from mature leaf explants of apple has been developed using micropropagated primary leaves of cv. Golden Delicious. The technique required second generation adventitious buds produced from cultured primary leaves also produced from es...

journal_title：Plant cell reports

authors： Rugini E,Muganu M

更新日期：1998-04-01 00:00:00

abstract：:An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just...

journal_title：Plant cell reports

authors： Esteves P,Belzile F

更新日期：2014-06-01 00:00:00

abstract：:Independent carrot (Daucus carota) hairy root lines were established by inoculation of discs taken from the same carrot with Agrobacterium rhizogenes 8196 and A. tumefaciens C58C1(pRi8196) carrying pRi8196. Several lines were compared with respect to T-DNA length. One of them was found to have integrated sequences cov...

journal_title：Plant cell reports

authors： David C,Petit A,Tempé J

更新日期：1988-03-01 00:00:00

abstract：:The introgression of new desirable characters is very necessary in common bean (Phaseolus vulgaris L.)Interspecific hybridization of P. vulgaris L. with P. angustissimus A. Gray, a wild species with narrow leaves, could be successfully achieved for the first time using embryo culture. Sixteen to twenty-three-day old e...

journal_title：Plant cell reports

authors： Belivanis T,Doré C

更新日期：1986-10-01 00:00:00

abstract：:Ginger (Zingiber officinale Rosc.) is prone to photoinhibition under intense sunlight. Excessive light can be dissipated by the xanthophyll cycle, where violaxanthin de-epoxidase (VDE) plays a critical role in protecting the photosynthesis apparatus from the damage of excessive light. We isolated ~2.0 kb of ginger VDE...

journal_title：Plant cell reports

authors： Zhao W,Wang S,Li X,Huang H,Sui X,Zhang Z

更新日期：2012-08-01 00:00:00

abstract：:Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or liquid medium gave s...

journal_title：Plant cell reports

authors： Senaratna T,Kott L,Beversdorf WD,McKersie BD

更新日期：1991-09-01 00:00:00

abstract：:Grapefruit (Citrus paradisi) tissue cultures were examined for qualitative and quantitative changes in flavanone-neohesperidoside content during somatic embryogenesis. Embryos cultured in vitro contain naringin and a rhamnosyl-transferase activity which is capable of rhamnosylating position 2 on the flavanone glucosid...

journal_title：Plant cell reports

authors： Gavish H,Lewinsohn E,Vardi A,Fluhr R

更新日期：1989-10-01 00:00:00

abstract：:In an attempt to improve growth and secondary metabolite production, and to understand the possible mechanism involved in relation to the changes in physiology and activities of antioxidant enzymes, we cultured Morinda citrifolia adventitious roots in different strength (0.25, 0.50, 0.75, 1.0, 1.5 and 2.0) of Murashig...

journal_title：Plant cell reports

authors： Abdullahil Baque M,Lee EJ,Paek KY

更新日期：2010-07-01 00:00:00

abstract：:Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana ( Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at e...

journal_title：Plant cell reports

authors： Helliot B,Swennen R,Poumay Y,Frison E,Lepoivre P,Panis B

更新日期：2003-03-01 00:00:00

abstract：:Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...

journal_title：Plant cell reports

authors： Kim ST,Kim SG,Hwang DH,Kang SY,Koo SC,Cho MJ,Kang KY

更新日期：2004-10-01 00:00:00

abstract：:The anthracnose fungus Colletotrichum gloeosporioides deleteriously affects unripe pepper fruit, but not ripe fruit. Here, we show that the induction of local acquired resistance (LAR) by salicylic acid (SA), 2,6-dichloroisonicotinic acid, or benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester pretreatment pro...

journal_title：Plant cell reports

authors： Lee S,Hong JC,Jeon WB,Chung YS,Sung S,Choi D,Joung YH,Oh BJ

更新日期：2009-10-01 00:00:00

abstract：:Several lipid classes constitute the universal matrix of the biological membranes. With their amphipathic nature, lipids not only build the continuous barrier that confers identity to every cell and organelle, but they are also active actors that modulate the activity of the proteins immersed in the lipid bilayer. The...

journal_title：Plant cell reports

authors： Morales-Cedillo F,González-Solís A,Gutiérrez-Angoa L,Cano-Ramírez DL,Gavilanes-Ruiz M

更新日期：2015-04-01 00:00:00

abstract：:In the present paper, attempts were made to explore the possibility of employing ultraviolet (UV) irradiation in citrus asymmetric fusion for transfer of limited amount of favorable traits from a desirable cultivar to a target one. Exposure of Satsuma mandarin (Citrus unshiu Marc.) embryogenic protoplasts to UV at an ...

journal_title：Plant cell reports

authors： Xu XY,Hu ZY,Li JF,Liu JH,Deng XX

更新日期：2007-08-01 00:00:00

abstract：KEY MESSAGE:MsCBL4 expression in tobacco enhanced its salt and saline-alkali stress tolerance by regulating calcium accumulation in roots, indicating the important role of calcium metabolism in plant saline-alkali stress tolerance The calcineurin B-like (CBL) family of proteins play important roles in plant abiotic str...

journal_title：Plant cell reports

authors： An Y,Yang XX,Zhang L,Zhang J,Du B,Yao L,Li XT,Guo C

更新日期：2020-08-01 00:00:00

abstract：KEY MESSAGE:The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection against Sclerotinia sclerotiorum and Rhizoctonia solani. Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoprot...

journal_title：Plant cell reports

authors： Beracochea VC,Almasia NI,Peluffo L,Nahirñak V,Hopp EH,Paniego N,Heinz RA,Vazquez-Rovere C,Lia VV

更新日期：2015-10-01 00:00:00

abstract：:We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck×Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We ...

journal_title：Plant cell reports

authors： Peña L,Cervera M,Juárez J,Navarro A,Pina JA,Navarro L

更新日期：1997-09-01 00:00:00

abstract：:Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which β-glucuronidase (GUS) is exp...

journal_title：Plant cell reports

authors： Kokko HI,Kärenlampi SO

更新日期：1998-07-01 00:00:00

abstract：:Arabinogalactan proteins (AGPs) are important proteoglycans regulating somatic embryogenesis in diverse plant species. Embryogenic cells of somatic embryos are covered by special extracellular cell wall layer called extracellular surface matrix network (ECMSN) at their early developmental stages. Here we show that hig...

journal_title：Plant cell reports

authors： Samaj J,Salaj T,Matúsová R,Salaj J,Takác T,Samajová O,Volkmann D

更新日期：2008-02-01 00:00:00

abstract：:We have isolated by affinity chromatography a lectin from the seeds of the winged bean (Psophocrapus tetragonolobus) which agglutinated human (group A, B and O), sheep and rabbit, but not mouse erythrocytes. A molecular weight of 41,000 was obtained from gel filtration, and on sodium dodecyl sulphate polyacrylamide ge...

journal_title：Plant cell reports

authors： Vijayakumar T,Forrester JA

更新日期：1986-12-01 00:00:00

abstract：KEY MESSAGE:When fused to " Pr AlSAP " promoter, transcripts of gusA exhibited similar accumulation patterns in transgenic rice as AlSAP transcripts in A. littoralis. Pr AlSAP can be used for engineering abiotic stress tolerance. We previously showed that ectopic expression of a stress-associated protein gene from Aelu...

journal_title：Plant cell reports

authors： Ben-Saad R,Meynard D,Ben-Romdhane W,Mieulet D,Verdeil JL,Al-Doss A,Guiderdoni E,Hassairi A

更新日期：2015-10-01 00:00:00

abstract：:Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...

journal_title：Plant cell reports

authors： Kim YH,Woloshuk CP,Cho EH,Bae JM,Song YS,Huh GH

更新日期：2007-04-01 00:00:00

abstract：:Sugarcane cell suspensions were initiated from leaf callus and sub-cultured every 7 to 10 days by alternate transfer to MS based medium with 3.0 and 1.0 mg 1(-1)2,4-D. Suspensions older than 3 months gave the most reproducible yields of protoplasts. Isolated protoplasts required 50 mM Ca(2+) in the washing solution an...

journal_title：Plant cell reports

authors： Chen WH,Davey MR,Power JB,Cocking EC

更新日期：1988-08-01 00:00:00

abstract：:Hypocotyl explants of three leguminous forest tree species, Albizia amara, A. lucida and A. richardiana, have differentiated shoot buds on B5 basal medium. Maximum number of shoots per explant developed on basal medium augmented with 2,4-D (0.1 μM) in A. amara (2) and BA (10 μM) for both A. lucida (2) and A. richardia...

journal_title：Plant cell reports

authors： Tomar UK,Gupta SC

更新日期：1988-10-01 00:00:00

abstract：:Transgenic calli of Pharbitis nil which grow in the presence of kanamycin were obtained by introduction of plasmid pBI121, which carries kanamycin resistance and the gene for beta-glucuronidase. The calli were shown to have fragments of vector DNA in their genome and high levels of beta-glucuronidase activity. This is...

journal_title：Plant cell reports

authors： Araki T,Hirano H,Naito S,Komeda Y

更新日期：1989-05-01 00:00:00

abstract：:A reliable and efficient system for transformation and regeneration of 'Chardonnay' (Vitis vinifera L.) plants via microprojectile bombardment was developed. Improvements over the previous biolistic transformation system included: (1) the use of gold particles for bombardment; (2) step-wise selection at 10 then 15 mg/...

journal_title：Plant cell reports

authors： Vidal JR,Kikkert JR,Wallace PG,Reisch BI

更新日期：2003-11-01 00:00:00

abstract：:Mitogen-activated protein kinase (MAPK) signaling cascades are evolutionarily conserved fundamental signal transduction pathways. A MAPK cascade consists of many distinct MAPKKK-MAPKK-MAPK modules linked to various upstream receptors and downstream targets through sequential phosphorylation and activation of the casca...

journal_title：Plant cell reports

authors： Singh R,Jwa NS

更新日期：2013-06-01 00:00:00

abstract：:An efficient culture system has been developed for repeated cycles of somatic embryogenesis in microspore-derived embryos of Brassica juncea without a callus phase. Haploid embryos produced through anther culture showed a high propensity for direct production of somatic embryos in response to 2 mgL(-1) BA and 0.1 mgL(...

journal_title：Plant cell reports

authors： Prabhudesai V,Bhaskaran S

更新日期：1993-03-01 00:00:00

abstract：: A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker ...

journal_title：Plant cell reports

authors： Nobre J,Davey MR,Lazzeri PA,Cannell ME

更新日期：2000-10-01 00:00:00