Cloning and functional expression of the gene encoding an inhibitor against Aspergillus flavus alpha-amylase, a novel seed lectin from Lablab purpureus (Dolichos lablab).


:Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is known to promote aflatoxin production in the endosperm of these infected kernels, and a 36-kDa protein from the Lablab purpureus, denoted AILP, has been shown to inhibit alpha-amylase production and the growth of A. flavus. Here, we report the isolation of six full-length labAI genes encoding AILP and a detailed analysis of the activities of the encoded proteins. Each of the six labAI genes encoded sequences of 274 amino acids, with the deduced amino acid sequences showing approximately 95-99% identity. The sequences are similar to those of lectin members of a legume lectin-arcelin-alpha-amylase inhibitor family reported to function in plant resistance to insect pests. The labAI genes did not show any of the structures characteristic of conserved structures identified in alpha-amylase inhibitors to date. The recombinant proteins of labAI-1 and labAI-2 agglutinated human red blood cells and inhibited A. flavus alpha-amylase in a manner similar to that shown by AILP. These data indicate that labAI genes are a new class of lectin members in legume seeds and that their proteins have both lectin and alpha-amylase inhibitor activity. These results are a valuable contribution to our knowledge of plant-pathogen interactions and will be applicable for developing protocols aimed at controlling A. flavus infection.


Plant Cell Rep


Plant cell reports


Kim YH,Woloshuk CP,Cho EH,Bae JM,Song YS,Huh GH




Has Abstract


2007-04-01 00:00:00












  • Shoot apex explants for induction of somatic embryogenesis in mature Quercus robur L. trees.

    abstract::A procedure for inducing somatic embryos in shoot apex explants (2 mm) excised from shoot proliferation cultures established from adult oak trees (Quercus robur) was investigated. Embryogenesis was induced in shoot tip as well as leaf explants in three out of the five genotypes evaluated. Somatic embryos were formed b...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: San-José MC,Corredoira E,Martínez MT,Vidal N,Valladares S,Mallón R,Vieitez AM

    更新日期:2010-06-01 00:00:00

  • Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl.

    abstract::Gastrodia elata Bl. is an achlorophyllous orchid plant feeding on the fungus Armillaria mellea. The plant lives underground during its life cycle except for florescence. Gastrodianins, members of the superfamily of monocot mannose-binding protein (MBP), have been identified from Gastrodia elata, yet their physiologica...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Wang HX,Yang T,Zeng Y,Hu Z

    更新日期:2007-03-01 00:00:00

  • Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1.

    abstract::Heading date in rice is an important agronomic trait controlled by several genes. In this study, flowering time of variety Dianjingyou 1 (DJY1) was earlier than a near-isogenic line (named NIL) carried chromosome segment from African rice on chromosome 3S, when grown in both long-day (LD) and short-day (SD) conditions...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Bian XF,Liu X,Zhao ZG,Jiang L,Gao H,Zhang YH,Zheng M,Chen LM,Liu SJ,Zhai HQ,Wan JM

    更新日期:2011-12-01 00:00:00

  • Genetic modification in Malaysia and India: current regulatory framework and the special case of non-transformative RNAi in agriculture.

    abstract::Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审


    authors: Darsan Singh JK,Mat Jalaluddin NS,Sanan-Mishra N,Harikrishna JA

    更新日期:2019-12-01 00:00:00

  • The tissue expression pattern of the AtGRP5 regulatory region is controlled by a combination of positive and negative elements.

    abstract::The AtGRP5 gene from Arabidopsis thaliana encodes a glycine-rich protein which has a major activity in protoderm-derived cells and is expressed in cells that undergo the first anatomical modifications leading to somatic embryo development. It has been previously demonstrated that its minimum promoter is 316 bp long in...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Mangeon A,Magioli C,Tarré E,Cardeal V,Araujo C,Falkenbach E,Rocha CA,Rangel-Lima C,Sachetto-Martins G

    更新日期:2010-05-01 00:00:00

  • Characterization of salt tolerant alfalfa (Medicago sativa L.) plants regenerated from salt tolerant cell lines.

    abstract::Salt tolerant cell lines have been selected from Medicago sativa, by a single step selection process on tissue culture medium containing 1% NaCl. Plants regenerated from these lines show improved salt tolerance compared to parent plants. The regenerated plants are vigorous, have flowered and are self fertile. The cell...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Winicov I

    更新日期:1991-12-01 00:00:00

  • Phytochelatin accumulation and stress tolerance in tomato cells exposed to cadmium.

    abstract::Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gupta SC,Goldsbrough PB

    更新日期:1990-12-01 00:00:00

  • Sugarcane white leaf phytoplasma in tissue culture: long-term maintenance, transmission, and oxytetracycline remission.

    abstract::Sugarcane white leaf (SCWL)-diseased sugarcane plants collected from Udornthani Province, in north-eastern Thailand, were the source for tissue culture experiments. Explants from axillary buds, meristem tips, and leaves grew optimally in Murashige-Skoog medium containing 0.5 mg/l alpha-naphthaleneacetic acid, 0.5 mg/l...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Wongkaew P,Fletcher J

    更新日期:2004-11-01 00:00:00

  • Agrobacterium induced gall formation in lipoxygenase mutant isolines of soybeans.

    abstract::Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos m...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Bhatt D,Parrott WA,Collins GB,Hildebrand DF

    更新日期:1991-03-01 00:00:00

  • Reversible variations in the methylation pattern of carrot DNA during somatic embryogenesis.

    abstract::Twenty five clones from a carrot genomic library were used as probes to detect variations in the 5-methyl-cytosine pattern during somatic embryogenesis. The majority of them evidenced an invariant pattern. With two clones however, differences were found between the adulttype DNA pattern and the embryonic one. The leve...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Vergara R,Verde F,Pitto L,Lo Schiavo F,Terzi M

    更新日期:1990-05-01 00:00:00

  • Characterization of promoter of EgPAL1, a novel PAL gene from the oil palm Elaeis guineensis Jacq.

    abstract:KEY MESSAGE:The oil palm EgPAL1 gene promoter and its regulatory region were functional as a promoter in the heterologous system of Arabidopsis according to the cis-acting elements present in that region. The promoter was developmentally regulated, vascular tissue specific and responsive to water stress agents. Phenyla...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Yusuf CYL,Abdullah JO,Shaharuddin NA,Abu Seman I,Abdullah MP

    更新日期:2018-02-01 00:00:00

  • Ultrastructural changes associated with cryopreservation of banana ( Musa spp.) highly proliferating meristems.

    abstract::Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana ( Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at e...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Helliot B,Swennen R,Poumay Y,Frison E,Lepoivre P,Panis B

    更新日期:2003-03-01 00:00:00

  • Efficient plantlet regeneration from protoplasts isolated from suspension cultures of poplar (Populus alba L.).

    abstract::We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 106 cells/g fresh weight cells and then cultured at a concentration of 2.5×105 cells/ml in NH4NO3-free Mu...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Qiao J,Kuroda H,Hayashi T,Sakai F

    更新日期:1998-01-01 00:00:00

  • Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice.

    abstract::Domain of Unknown Function 966 (DUF966) gene family was found in the protein family database, which consisted of seven genes in rice. The proteins encoded by these genes contained one or two highly conserved DUF966 domains. The available data of public microarray databases implied that these genes might play crucial r...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Luo C,Guo C,Wang W,Wang L,Chen L

    更新日期:2014-02-01 00:00:00

  • Glutathione homeostasis and Cd tolerance in the Arabidopsis sultr1;1-sultr1;2 double mutant with limiting sulfate supply.

    abstract:KEY MESSAGE:Cadmium sensitivity in sultr1;1 - sultr1;2 double mutant with limiting sulfate supply is attributed to the decreased glutathione content that affected oxidative defense but not phytochelatins' synthesis. In plants, glutathione (GSH) homeostasis plays pivotal role in cadmium (Cd) detoxification. GSH is synth...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Liu X,Wu FH,Li JX,Chen J,Wang GH,Wang WH,Hu WJ,Gao LJ,Wang ZL,Chen JH,Simon M,Zheng HL

    更新日期:2016-02-01 00:00:00

  • Genotype-specific heat shock proteins in two maize inbreds.

    abstract::Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Jorgensen JA,Weng J,Ho TH,Nguyen HT

    更新日期:1992-10-01 00:00:00

  • Plant regeneration from immature embryos of peanut.

    abstract::Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ozias-Akins P

    更新日期:1989-04-01 00:00:00

  • The pollen- and embryo-specific Arabidopsis DLL promoter bears good potential for application in marker-free Cre/loxP self-excision strategy.

    abstract:KEY MESSAGE:Marker-free transgenic plants can be generated with high efficiency by using the Cre/ lox P self-excision system controlled by the pollen- and embryo-specific Arabidopsis DLL promoter. In this work, we aimed to study the feasibility of using the pollen- and embryo-specific DLL promoter of the At4g16160 gene...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Polóniová Z,Jopčík M,Matušíková I,Libantová J,Moravčíková J

    更新日期:2015-03-01 00:00:00

  • The ornamental variety, Japanese striped corn, contains high anthocyanin levels and PAL specific activity: establishing the potential for development of an oral therapeutic.

    abstract::Phenylalanine ammonia-lyase [PAL, EC (formerly EC], functions in the plant phenylpropanoid biosynthetic pathway to deaminate the amino acid L-phenylalanine forming trans-cinnamic acid and ammonia. The human inherited metabolic disorder phenylketonuria (PKU) is characterized by an inability of individ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: McInnis S,Clemens S,Kermode AR

    更新日期:2009-03-01 00:00:00

  • Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana.

    abstract:KEY MESSAGE:The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection against Sclerotinia sclerotiorum and Rhizoctonia solani. Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoprot...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Beracochea VC,Almasia NI,Peluffo L,Nahirñak V,Hopp EH,Paniego N,Heinz RA,Vazquez-Rovere C,Lia VV

    更新日期:2015-10-01 00:00:00

  • Vegetative propagation of Quercus suber L. by somatic embryogenesis. II. Plant regeneration from selected cork oak trees.

    abstract::The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segmen...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hernández I,Celestino C,Alegre J,Toribio M

    更新日期:2003-04-01 00:00:00

  • Rapid micropropagation for Mucuna pruriens f. pruriens L.

    abstract::A rapid micropropagation system was developed for Mucuna pruriens f. pruriens using explants from 1-week-old aseptically grown seedlings. Multiple shoot regeneration occurred following an initial callus growth on Revised Tobacco (RT) medium supplemented with 2.7 μM NAA and 9.8 μM 2iP. Maximum number of shoot regenerat...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Chattopadhyay S,Datta SK,Mahato SB

    更新日期:1995-12-01 00:00:00

  • Does pretreatment by rhodamine 6-G affect the mitochondrial composition of fusion-derived Nicotiana cybrids?

    abstract::Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Aviv D,Chen R,Galun E

    更新日期:1986-06-01 00:00:00

  • Establishment of embryogenic cell suspension cultures of garlic (Allium sativum L.), plant regeneration and biochemical analyses.

    abstract::Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1-4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophen...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Fereol L,Chovelon V,Causse S,Triaire D,Arnault I,Auger J,Kahane R

    更新日期:2005-08-01 00:00:00

  • Genome-wide transcriptional response of papain-like cysteine protease-mediated resistance against Xanthomonas oryzae pv. oryzae in rice.

    abstract:KEY MESSAGE:Transgenic rice overexpressing PLCP attenuated the virulence of Xanthomonas oryzae pv. oryzae through extensive activation of transduction signal and transcription activities that orchestrate downstream responses including the biosynthesis of secondary metabolites and up-regulation of several pathogenesis-r...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Niño MC,Kang KK,Cho YG

    更新日期:2020-04-01 00:00:00

  • Purple-leaved Ficus lyrata plants produced by overexpressing a grapevine VvMybA1 gene.

    abstract:KEY MESSAGE:This study established an efficient method of regenerating plants of Ficus lyrata and producing purple-leaved F. lyrata plants through genetic transformation using a VvMybA1 gene of grapevine. ABSTRACT:Ficus lyrata, a species with unique violin- or guitar-shaped leaves, was regenerated from leaf-derived ca...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Zhao J,Li ZT,Chen J,Henny RJ,Gray DJ,Chen J

    更新日期:2013-11-01 00:00:00

  • Functional divergence of GhCFE5 homoeologs revealed in cotton fiber and Arabidopsis root cell development.

    abstract:KEY MESSAGE:In GhCFE5 homoeologs, GhCFE5D interacted with more actin homologs and stronger interaction activity than GhCFE5A. GhCFE5D - but not GhCFE5A -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. Homoeologous genes are common in polyploid plants; howe...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Lv F,Li P,Zhang R,Li N,Guo W

    更新日期:2016-04-01 00:00:00

  • The Arabidopsis thaliana hypocotyl, a model to identify and study control mechanisms of cellular expansion.

    abstract::Developmental biology studies in general benefit from model organisms that are well characterized. Arabidopsis thaliana fulfills this criterion and represents one of the best experimental systems to study developmental processes in higher plants. Light is a crucial factor that drives photosynthesis, but that also regu...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审


    authors: Boron AK,Vissenberg K

    更新日期:2014-05-01 00:00:00

  • Micropropagation of Vitex negundo L., a woody aromatic medicinal shrub, through high-frequency axillary shoot proliferation.

    abstract::A protocol is described for rapid and large-scale propagation of the woody aromatic and medicinal shrub Vitex negundo by in vitro culture of nodal segments from mature plants. Of the three different cytokinins - N6-benzyladenine (BA), kinetin, and thidiazuron - evaluated as supplements to Murashige and Skoog (MS) medi...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Sahoo Y,Chand PK

    更新日期:1998-12-01 00:00:00

  • Effects of cadmium stress on the antioxidant system and chlorophyll fluorescence characteristics of two Taxodium clones.

    abstract:KEY MESSAGE:The T.118 and T.406 seedlings showed strong adaptability under Cd concentrations ≤ 50 µM. The mechanisms of photoprotection in T.118 and T.406 differed in high-Cd concentrations. To explore the physiological response characteristics of Taxodium hybrids to cadmium (Cd) stress and provide basis for screening ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ma YL,Wang HF,Wang P,Yu CG,Luo SQ,Zhang YF,Xie YF

    更新日期:2018-11-01 00:00:00