Abstract：
:Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is known to promote aflatoxin production in the endosperm of these infected kernels, and a 36-kDa protein from the Lablab purpureus, denoted AILP, has been shown to inhibit alpha-amylase production and the growth of A. flavus. Here, we report the isolation of six full-length labAI genes encoding AILP and a detailed analysis of the activities of the encoded proteins. Each of the six labAI genes encoded sequences of 274 amino acids, with the deduced amino acid sequences showing approximately 95-99% identity. The sequences are similar to those of lectin members of a legume lectin-arcelin-alpha-amylase inhibitor family reported to function in plant resistance to insect pests. The labAI genes did not show any of the structures characteristic of conserved structures identified in alpha-amylase inhibitors to date. The recombinant proteins of labAI-1 and labAI-2 agglutinated human red blood cells and inhibited A. flavus alpha-amylase in a manner similar to that shown by AILP. These data indicate that labAI genes are a new class of lectin members in legume seeds and that their proteins have both lectin and alpha-amylase inhibitor activity. These results are a valuable contribution to our knowledge of plant-pathogen interactions and will be applicable for developing protocols aimed at controlling A. flavus infection.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Kim YH,Woloshuk CP,Cho EH,Bae JM,Song YS,Huh GHdoi
10.1007/s00299-006-0250-2subject
Has Abstractpub_date
2007-04-01 00:00:00pages
395-405issue
4eissn
0721-7714issn
1432-203Xjournal_volume
26pub_type
杂志文章abstract：:The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated b...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232217
更新日期：1996-06-01 00:00:00
abstract：:Cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. were found to produce a large amount of berberine (400-800 mg/l) when 5-10 μM 6-benzyladenine was added to Linsmaier and Skoog's medium containing 100 μM 1-naphthaleneacetic acid. Most of the berberine produced was released continuously from the cell...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269306
更新日期：1984-12-01 00:00:00
abstract：KEY MESSAGE:An ERF gene, LchERF , was cloned from L. chinense for the first time. Overexpression of LchERF conferred salt stress tolerance in transgenic tobacco lines during seed germination and vegetative growth. Ethylene-responsive transcription factors (ERFs) play important roles in tolerance to biotic and abiotic s...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1678-4
更新日期：2014-12-01 00:00:00
abstract：KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2184-2
更新日期：2017-11-01 00:00:00
abstract：:The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf dis...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-002-0530-4
更新日期：2003-01-01 00:00:00
abstract：:Protoplasts were isolated from seedling roots, hypocotyls, and cotyledons of four cultivars of Helianthus annuus and from leaves of axenic shoot cultures of the wild species H. praecox, H. scaberimus and H. rigidus. Optimal culture conditions were established for the respective protoplast systems, using the agarose be...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269815
更新日期：1986-08-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269711
更新日期：1986-02-01 00:00:00
abstract：:Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl e...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0695-1
更新日期：2009-06-01 00:00:00
abstract：:In long-term callus cultures of Cyclamen persicum Mill. two types of tissue could be distinguished. One type featured a brown suberised outer layer and was poorly organogenic. The other type was yellowish in appearance and gave rise to many shoot buds. Both types co-existed on the same callus, the former prevailing. S...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232991
更新日期：1996-03-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1491-5
更新日期：2013-11-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235354
更新日期：1991-04-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233152
更新日期：1996-08-01 00:00:00
abstract：: The potential of ultrasonic standing waves for trapping suspended particles was utilized successfully for differential size fractionation of plant somatic embryos. In a flow-through resonator equipped with a 200-kHz piezoceramic transducer, embryos of different sizes corresponding to different developmental stages co...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000234
更新日期：2000-11-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0854-4
更新日期：2010-07-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02465-3
更新日期：2019-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0937-9
更新日期：2005-08-01 00:00:00
abstract：:Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1164-1
更新日期：2012-02-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050364
更新日期：1997-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269273
更新日期：1981-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-013-1437-y
更新日期：2013-06-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0230-6
更新日期：2007-03-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0667-x
更新日期：2009-04-01 00:00:00
abstract：KEY MESSAGE:High and low resveratrol (Res) contents in two cultivars are correlated with the expression abundance of Myb14 , which could directly activate transcriptional expression of stilbene synthase gene ( STS ). Resveratrol (3,5,4'-trihydroxystilbene) is one of the natural polyphenols produced by secondary metabol...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1642-3
更新日期：2014-10-01 00:00:00
abstract：:Three different regeneration systems, viz. direct regeneration of adventitious shoot buds from explant, regeneration through callus cultures and somatic embryos were compared to see their effect on transfer of neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) reporter gene (gus) to Morus alba clone M5, ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0217-3
更新日期：2007-02-01 00:00:00
abstract：:Euphorbia dulcis endosperm is the site of controlled long lasting endocellular lysis involving segregation and autophagy of portions of the cytoplasm within the endoplasmic reticulum membrane. The lysis products may constitute a food source for the benefit of the early developing embryo. ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231924
更新日期：1996-05-01 00:00:00
abstract：:The production of cybrids, combining nucleus of a species with alien cytoplasmic organelles, is a valuable method used for improvement of various crops. Several citrus cybrids have been created by somatic hybridization. These genotypes are interesting models to analyze the impact of cytoplasmic genome change on nuclea...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1014-1
更新日期：2011-06-01 00:00:00
abstract：:The Golgi apparatus in plants is organized as a multitude of individual stacks that are motile in the cytoplasm and in close association with the endoplasmic reticulum (ER) (Boevink et al. in Plant J 15:441-447, 1998). These stacks operate as a sorting centre for cargo molecules, providing modification and redirection...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-007-0390-z
更新日期：2007-09-01 00:00:00
abstract：:Arabinogalactan proteins (AGPs) are important proteoglycans regulating somatic embryogenesis in diverse plant species. Embryogenic cells of somatic embryos are covered by special extracellular cell wall layer called extracellular surface matrix network (ECMSN) at their early developmental stages. Here we show that hig...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0429-1
更新日期：2008-02-01 00:00:00
abstract：: Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-old nodular green ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050763
更新日期：2000-04-01 00:00:00
abstract：:Callus derived from stem segments of loblolly pine (Pinus taeda L.) was subcultured to media containing 0.5% of various mono-, di-, tri- or polysaccharides. None of the carbohydrate sources tested were superior to sucrose. Growth on twelve of the 20 carbohydrates tested was more than 75% of that obtained for sucrose; ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00276676
更新日期：1987-04-01 00:00:00
