Cloning and functional expression of the gene encoding an inhibitor against Aspergillus flavus alpha-amylase, a novel seed lectin from Lablab purpureus (Dolichos lablab).

abstract：:Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which β-glucuronidase (GUS) is exp...

journal_title：Plant cell reports

authors： Kokko HI,Kärenlampi SO

更新日期：1998-07-01 00:00:00

abstract：KEY MESSAGE:MsCBL4 expression in tobacco enhanced its salt and saline-alkali stress tolerance by regulating calcium accumulation in roots, indicating the important role of calcium metabolism in plant saline-alkali stress tolerance The calcineurin B-like (CBL) family of proteins play important roles in plant abiotic str...

journal_title：Plant cell reports

authors： An Y,Yang XX,Zhang L,Zhang J,Du B,Yao L,Li XT,Guo C

更新日期：2020-08-01 00:00:00

abstract：KEY MESSAGE:The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection against Sclerotinia sclerotiorum and Rhizoctonia solani. Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoprot...

journal_title：Plant cell reports

authors： Beracochea VC,Almasia NI,Peluffo L,Nahirñak V,Hopp EH,Paniego N,Heinz RA,Vazquez-Rovere C,Lia VV

更新日期：2015-10-01 00:00:00

abstract：:A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-d...

journal_title：Plant cell reports

authors： Bakshi S,Saha B,Roy NK,Mishra S,Panda SK,Sahoo L

更新日期：2012-06-01 00:00:00

abstract：: The influences of light conditions, sucrose and ethylene on in vitro formation and storability of onion (Allium cepa L.) bulblets were studied in various accessions. Light, sucrose and ethylene influenced bulb formation. Storability was primarily enhanced by a high sucrose concentration (100 g/l) in the culture mediu...

journal_title：Plant cell reports

authors： Kästner U,Klahr A,Keller ERJ,Kahane R

更新日期：2001-02-01 00:00:00

abstract：KEY MESSAGE:A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineeri...

journal_title：Plant cell reports

authors： Li J,Xu RF,Qin RY,Ma H,Li H,Zhang YP,Li L,Wei PC,Yang JB

更新日期：2014-10-01 00:00:00

abstract：:Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...

journal_title：Plant cell reports

authors： Aviv D,Chen R,Galun E

更新日期：1986-06-01 00:00:00

abstract：:This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...

journal_title：Plant cell reports

authors： Caillot S,Rosiau E,Laplace C,Thomasset B

更新日期：2009-03-01 00:00:00

abstract：:Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...

journal_title：Plant cell reports

authors： Moreno OA,Vazquez-Duhalt R,Nolasco H

更新日期：1990-07-01 00:00:00

abstract：:The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...

journal_title：Plant cell reports

authors： Prabu G,Prasad DT

更新日期：2012-04-01 00:00:00

abstract：:Protoplasts derived from oat (Avena sativa L.) suspension culture cells (7 days after subculturing) were electroporated with plasmid DNA containing the Escherichia coli uidA gene encoding the ß-glucuronidase reporter enzyme. Consistently high enzyme activity was observed with electroporation conditions of 500 μF and 1...

journal_title：Plant cell reports

authors： Higgs DC,Colbert JT

更新日期：1993-05-01 00:00:00

abstract：:A new method of estimating the amount of berberine released from minute cell colonies of Thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. In this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and ...

journal_title：Plant cell reports

authors： Suzuki T,Yoshioka T,Hara Y,Tabata M,Fujita Y

更新日期：1987-06-01 00:00:00

abstract：KEY MESSAGE:Drastic changes in soil water content can activate the short-term high expression of key enzyme-encoding genes involved in secondary metabolite synthesis thereby increasing the content of secondary metabolites. Bupleurum chinense DC. is a traditional medicinal herb that is famous for its abundant saikosapon...

journal_title：Plant cell reports

authors： Yang L,Zhao Y,Zhang Q,Cheng L,Han M,Ren Y,Yang L

更新日期：2019-09-01 00:00:00

abstract：:Spermidine and ornithine given to normal auxin-requiring cell suspensions of sugarbeet inhibited peroxidase secretion in the absence of Ca(2+). Habituated (organogenic or not) cells did not respond. Both compounds counteracted the Ca(2+) - promoted enzyme secretion by three cell lines. Auxins (2,4-D and BSAA) did not ...

journal_title：Plant cell reports

authors： Kevers C,Gaspar T,Penel C,Greppin H

更新日期：1985-05-01 00:00:00

abstract：:A NaCl-tolerant callus line of Cicer arietinum has been isolated, as a spontaneous variant, on agar-solidified MS-medium supplemented with 100 mM NaCl. The growth of this line, in the presence of 100 mM NaCl, was comparable to that of the NaCl-sensitive callus line growing in the absence of NaCl. Regarding relative to...

journal_title：Plant cell reports

authors： Pandey R,Ganapathy PS

更新日期：1984-04-01 00:00:00

abstract：:Most infections of plant roots are initiated in the region of elongation; the mechanism for this tissue-specific localization pattern is unknown. In alfalfa expressing PsUGT1 antisense mRNA under the control of the cauliflower mosaic virus (CaMV) 35S promoter, the cell cycle in roots is completed in 48 h instead of 24...

journal_title：Plant cell reports

authors： Woo HH,Hirsch AM,Hawes MC

更新日期：2004-07-01 00:00:00

abstract：:A microscopical study was performed in order to localize chromogenic valepotriates in roots (in vivo) and in callus and root organ cultures (in vitro) of valerian plants. These in vitro cultures (producing valepotriates) contained lipid droplets having the same aspect as those containing essential oil described in the...

journal_title：Plant cell reports

authors： Violon C,Dekegel D,Vercruysse A

更新日期：1983-12-01 00:00:00

abstract：:Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...

journal_title：Plant cell reports

authors： Skirvin RM,Chu MC,Mann ML,Young H,Sullivan J,Fermanian T

更新日期：1986-08-01 00:00:00

abstract：:Treatment of Allium cepa L. cellsuspension cultures with a biotic elicitor derived from the fungus Botrytis cinerea, resulted in phytoalexin synthesis. Two phytoalexins, 5-octylcyclopenta-1,3-dione and 5-hexyl-cyclopenta-1,3-dione, were accumulated in cultured onion cells. Removal of extracellular Ca(2+) by the calciu...

journal_title：Plant cell reports

authors： Dmitriev A,Djatsok J,Grodzinsky D

更新日期：1996-09-01 00:00:00

abstract：:DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...

journal_title：Plant cell reports

authors： Nelke M,Nowak J,Wright JM,McLean NL

更新日期：1993-12-01 00:00:00

abstract：:It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, ...

journal_title：Plant cell reports

authors： Manzocchi LA,Bianchi MW,Viotti A

更新日期：1989-03-01 00:00:00

abstract：:Fertile transgenic barley (Hordeum vulgare L.) plants were obtained by high velocity particle bombardment. The plasmid pBCl was used to deliver the selectable hph gene and reporter Gus gene into immature embryo. After the selection culture 18 hygromycin resistant plants were obtained. Samples for Southern hybridizatio...

journal_title：Plant cell reports

authors： Hagio T,Hirabayashi T,Machii H,Tomotsune H

更新日期：1995-02-01 00:00:00

abstract：:Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...

journal_title：Plant cell reports

authors： Moore PJ,Moore AJ,Collins GB

更新日期：1994-07-01 00:00:00

abstract：:A test system for root regeneration was developed that consists of stem slices of apple shoots (ca. 0.5 mm thick; fresh weight ca. 1 mg). Roots regenerated synchronously without intervening callus formation and without interference with compounds originating from other parts of the plant. Supply of indolebutyric acid ...

journal_title：Plant cell reports

authors： Van der Krieken WM,Breteler H,Visser MH,Mavridou D

更新日期：1993-02-01 00:00:00

abstract：:Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced...

journal_title：Plant cell reports

authors： Lee-Parsons CW,Royce AJ

更新日期：2006-06-01 00:00:00

abstract：:Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of a...

journal_title：Plant cell reports

authors： Pietrosiuk A,Sykłowska-Baranek K,Wiedenfeld H,Wolinowska R,Furmanowa M,Jaroszyk E

更新日期：2006-10-01 00:00:00

abstract：KEY MESSAGE:We isolated differentially expressed and dark-responsive genes during flower development and opening in petals of morning glory. Flower opening usually depends on petal expansion and is regulated by both genetic and environmental factors. Flower opening in morning glory (Ipomoea nil) is controlled by the da...

journal_title：Plant cell reports

authors： Shinozaki Y,Tanaka R,Ono H,Ogiwara I,Kanekatsu M,van Doorn WG,Yamada T

更新日期：2014-07-01 00:00:00

abstract：:Embryogenic callus and suspension cultures of carrot (Daucus carota L., cv. Nantaise), growing on/in medium including 1 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), were transferred to medium with or without this plant growth regulator, to impair or induce, respectively, further development of somatic embryos. The en...

journal_title：Plant cell reports

authors： Jiménez VM,Guevara E,Herrera J,Bangerth F

更新日期：2005-01-01 00:00:00

abstract：:In leek (Allium ampeloprasum L.) a cyclic system of somatic embryogenesis was developed. Somatic embryos used for cyclic embryogenesis were able to develop the same type of embryogenic callus as zygotic embryos in the primary cycle. For the first time a comparison of the efficiencies of both expiants was made. Ten fam...

journal_title：Plant cell reports

authors： Schavemaker CM,Jacobsen E

更新日期：1995-01-01 00:00:00

abstract：:Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...

journal_title：Plant cell reports

authors： Zhang JJ,Shu QY,Liu ZA,Ren HX,Wang LS,De Keyser E

更新日期：2012-02-01 00:00:00