Abstract：
:Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is known to promote aflatoxin production in the endosperm of these infected kernels, and a 36-kDa protein from the Lablab purpureus, denoted AILP, has been shown to inhibit alpha-amylase production and the growth of A. flavus. Here, we report the isolation of six full-length labAI genes encoding AILP and a detailed analysis of the activities of the encoded proteins. Each of the six labAI genes encoded sequences of 274 amino acids, with the deduced amino acid sequences showing approximately 95-99% identity. The sequences are similar to those of lectin members of a legume lectin-arcelin-alpha-amylase inhibitor family reported to function in plant resistance to insect pests. The labAI genes did not show any of the structures characteristic of conserved structures identified in alpha-amylase inhibitors to date. The recombinant proteins of labAI-1 and labAI-2 agglutinated human red blood cells and inhibited A. flavus alpha-amylase in a manner similar to that shown by AILP. These data indicate that labAI genes are a new class of lectin members in legume seeds and that their proteins have both lectin and alpha-amylase inhibitor activity. These results are a valuable contribution to our knowledge of plant-pathogen interactions and will be applicable for developing protocols aimed at controlling A. flavus infection.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Kim YH,Woloshuk CP,Cho EH,Bae JM,Song YS,Huh GHdoi
10.1007/s00299-006-0250-2subject
Has Abstractpub_date
2007-04-01 00:00:00pages
395-405issue
4eissn
0721-7714issn
1432-203Xjournal_volume
26pub_type
杂志文章abstract：:A procedure for inducing somatic embryos in shoot apex explants (2 mm) excised from shoot proliferation cultures established from adult oak trees (Quercus robur) was investigated. Embryogenesis was induced in shoot tip as well as leaf explants in three out of the five genotypes evaluated. Somatic embryos were formed b...
journal_title：Plant cell reports
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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doi：10.1007/s00299-010-0835-7
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journal_title：Plant cell reports
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journal_title：Plant cell reports
pub_type： 杂志文章
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journal_title：Plant cell reports
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1007/s00299-013-1532-0
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pub_type： 杂志文章
doi：10.1007/s00299-015-1892-8
更新日期：2016-02-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233096
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journal_title：Plant cell reports
pub_type： 杂志文章
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journal_title：Plant cell reports
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Plant cell reports
pub_type： 杂志文章,评审
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更新日期：2014-05-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
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更新日期：1998-12-01 00:00:00
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