Abstract:
: The influences of light conditions, sucrose and ethylene on in vitro formation and storability of onion (Allium cepa L.) bulblets were studied in various accessions. Light, sucrose and ethylene influenced bulb formation. Storability was primarily enhanced by a high sucrose concentration (100 g/l) in the culture medium. The bulbing process was characterised by changes in bulbing ratio, leaf length, number of leaves and leaf development time. The viability of bulbs after 1 year of in vitro storage at low temperatures was determined by their growth reaction in subsequent subcultures, growth after transfer into the greenhouse and tetrazolium staining. Sufficient sprouting of bulblets previously stored at -1 °C demonstrated the possibility of storing them in a low-temperature, slow-growth culture.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Kästner U,Klahr A,Keller ERJ,Kahane Rdoi
10.1007/s002990000295subject
Has Abstractpub_date
2001-02-01 00:00:00pages
137-142issue
2eissn
0721-7714issn
1432-203Xpii
10.1007/s002990000295journal_volume
20pub_type
杂志文章abstract:KEY MESSAGE:Our studies indicate a potential important elicitor candidate which can aid in the fight against a worldwide disease, rice blast. In this study, we report the purification, identification, characterization, and gene cloning of a novel hypersensitive response-inducing protein elicitor (MoHrip2) secreted from...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1663-y
更新日期:2014-11-01 00:00:00
abstract::A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedli...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272760
更新日期:1988-12-01 00:00:00
abstract::A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protop...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050470
更新日期:1998-06-01 00:00:00
abstract::Diarrheal diseases caused by Vibrio cholerae and enterotoxigenic Escherichia coli (ETEC) are worldwide health problems that might be prevented with vaccines based on edible plants expressing the B subunit from either the cholera toxin (CTB) or the E. coli heat labile toxin (LTB). In this work we analyzed the immunity ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0439-z
更新日期:2008-01-01 00:00:00
abstract::In an effort to develop a chemically inducible system for insect management, we studied production of Cry1Ab Bacillus thuringiensis (Bt) protein and control of the diamondback moth (DBM), Plutella xylostella L., in inducer-treated and untreated tissues of a broccoli line transformed with a PR-1a/cry1Ab expression cass...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0091-4
更新日期:2006-06-01 00:00:00
abstract:UNLABELLED:The quality and productivity of chrysanthemum are severely compromised by various abiotic stresses. Here, we describe the isolation of CdICE1 from Chrysanthemum dichrum using RACE PCR, which shared identical nucleotide of ICE1 ORF from Chrysanthemum grandiflorum variety 'Jinba'. CdICE1 contains a conserved b...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1288-y
更新日期:2012-09-01 00:00:00
abstract::DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00235293
更新日期:1993-12-01 00:00:00
abstract::In leek (Allium ampeloprasum L.) a cyclic system of somatic embryogenesis was developed. Somatic embryos used for cyclic embryogenesis were able to develop the same type of embryogenic callus as zygotic embryos in the primary cycle. For the first time a comparison of the efficiencies of both expiants was made. Ten fam...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233638
更新日期:1995-01-01 00:00:00
abstract::Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232160
更新日期:1992-04-01 00:00:00
abstract::A rapid micropropagation system was developed for Mucuna pruriens f. pruriens using explants from 1-week-old aseptically grown seedlings. Multiple shoot regeneration occurred following an initial callus growth on Revised Tobacco (RT) medium supplemented with 2.7 μM NAA and 9.8 μM 2iP. Maximum number of shoot regenerat...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193734
更新日期:1995-12-01 00:00:00
abstract:UNLABELLED:Musa acuminata ssp. burmannica, one of the wild progenitors contributing 'A genome' to the present-day dessert bananas, has a long evolutionary history intervened by human activities. In this study, ISSR markers were used to analyze the pattern of genetic variation and differentiation in 32 individuals along...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1273-5
更新日期:2012-09-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational inhibition of their target mRNAs. Populus euphratica is a typical stress-resistant sessile organism that grows in desert areas. Here, we identified sequences of 12 miRNA precursors from 11...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1096-9
更新日期:2011-10-01 00:00:00
abstract::Ginger (Zingiber officinale Rosc.) is prone to photoinhibition under intense sunlight. Excessive light can be dissipated by the xanthophyll cycle, where violaxanthin de-epoxidase (VDE) plays a critical role in protecting the photosynthesis apparatus from the damage of excessive light. We isolated ~2.0 kb of ginger VDE...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1254-8
更新日期:2012-08-01 00:00:00
abstract::This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that wer...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0687-5
更新日期:2003-11-01 00:00:00
abstract::Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1164-1
更新日期:2012-02-01 00:00:00
abstract::The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01890855
更新日期:1996-12-01 00:00:00
abstract::In vitro culture of pineapple (Ananas comosus) was studied to determine the efficiency of axillary bud culture for rapid propagation of several cultivars. The technique used maximizes the success rate of various steps in the production of pineapple plants. Rapid mass multiplication of plantlets started 9 months after ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272750
更新日期:1988-12-01 00:00:00
abstract::Self-incompatibility (SI) discriminating self- and non-self pollen is regulated by S-locus genes in Brassica. In most of the S haplotypes, a highly polymorphic S-locus glycoprotein ( SLG) gene is tightly linked to genes for the SI determinants, S-receptor kinase ( SRK) and SP11, although the precise function of SLG in...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0598-5
更新日期:2003-06-01 00:00:00
abstract::Pigeon pea [Cajanus cajan (L.) Millsp.] (Family: Fabaceae) is an important legume crop cultivated across 50 countries in Asia, Africa, and the Americas; and ranks fifth in area among pulses after soybean, common bean, peanut, and chickpea. It is consumed as a major source of protein (21%) to the human population in ma...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-010-0899-4
更新日期:2010-10-01 00:00:00
abstract::Expression of the green fluorescent protein (gfp) gene, under regulatory control of either the constitutive 35S promoter or the developmentally-regulated lectin promoter was monitored and quantified using a newly-developed automated tracking system. The automated system consisted of a computer-controlled two-dimension...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0142-5
更新日期:2006-09-01 00:00:00
abstract::Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(me...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0621-x
更新日期:2003-08-01 00:00:00
abstract::Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232608
更新日期:1991-10-01 00:00:00
abstract:KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1773-1
更新日期:2015-07-01 00:00:00
abstract:KEY MESSAGE:The Arabidopsis AP2/ERF family transcription factor AtERF15 is nuclear-localized and positively regulates ABA and stress responses. Abscisic acid (ABA) is a major plant hormone that controls the expression of hundreds genes involved in various aspects of plant growth and development, such as seed developmen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1688-2
更新日期:2015-01-01 00:00:00
abstract::The non-yellowing gene (NYE1), initially identified from Arabidopsis, is a key regulatory gene responsible for chlorophyll degradation during senescence. Here, FaNYE1, an orthologue of AtNYE1, was further identified from a major type of cool-season turf grass, tall fescue (Festuca arundinacea Schreb.), by RACE-PCR. It...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1028-8
更新日期:2011-07-01 00:00:00
abstract::Two modes of endocytosis are known to occur in eucaryotic cells: fluid phase and receptor-mediated endocytosis. Fluid-phase endocytosis in plant cells resembles the retrieval of excess plasma membrane material previously incorporated by exocytosis. Pollen tubes need to carry out strong membrane retrieval due to their ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0437-1
更新日期:2008-01-01 00:00:00
abstract:: An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. T...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050743
更新日期:2000-03-01 00:00:00
abstract::We have developed a plant- Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selec...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0792-0
更新日期:2004-08-01 00:00:00
abstract::Callus cultures from cotyledon explants were established and maintained in culture for more than two years. After several months callus cultures were transferred into liquid medium and cultured as cell suspensions. Protoplasts were isolated from these cell suspension cultures and cultured in a liquid medium. After for...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269184
更新日期:1982-06-01 00:00:00
abstract::The nuclear DNA content of sweet pepper (Capsicum annuum L. var. annuum, 2n = 24) has been measured by flow and image cytometries but the DNA content of each chromosome of this species has not yet been regarded. DNA content of individual chromosomes has been quantified by the flow karyotyping technique, which requires...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0539-4
更新日期:2008-07-01 00:00:00