In planta 2,3,5 truodobenzoic acid treatment promotes high frequency and routine in vitro regeneration of sugarbeet (Beta vulgaris L.) plants.

Abstract:

:The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from cultured leaf explants. Without this treatment, no shoot developed on the control leaf-calluses. Several hundred plants were routinely regenerated using this protocol. More importantly, the number of shoots per explantcallus increased drastically over the subsequent subculture period. The most favorable media for callus induction contained a combination of an auxin and a cytokinin (0.1 mg/1 2,4-dichlorophenoxyacetic acid and 1 mg/1 N-6 benzylaminopurine) or a cytokinin alone (2.2 mg/1 thidiazuron). However, only the callus derived from leaves of TIBA-treated genotypes and induced on thidiazuron-medium produced numerous shoots. Histological studies showed the formation of meristematic zones only in the organogenic callus developed on thidiazuron-coutaining medium. The analysis of peroxidase activity showed that the activity was higher for the TIBA-treated plants than for the untreated control plants.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Roussy I,Dubois F,Sangwan RS,Sangwan-Norreel BS

doi

10.1007/BF01890855

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

142-6

issue

3-4

eissn

0721-7714

issn

1432-203X

journal_volume

16

pub_type

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