Abstract:
:The first efficient cryopreservation procedure for in vitro-grown shoot tips of raspberry (Rubus idaeus L.) has been developed based on encapsulation-vitrification (EnVi) and encapsulation-dehydration (EnDe). EnVi resulted in higher survival (85%) and regrowth (75%) of cryopreserved shoot tips than EnDe (65 and 50%, respectively). In both cryogenic procedures, shoots regenerated from cryopreserved shoot tips without intermediary callus formation. Histological studies showed that a much larger number of meristematic cells survived following EnVi than EnDe. The EnVi procedure was applied to seven raspberry genotypes with an average survival and regrowth of 71 and 68%, respectively. Regenerated plants showed normal morphology. Results here indicate EnVi as a simple and efficient method for long-term preservation of R. idaeus germplasm.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Wang Q,Laamanen J,Uosukainen M,Valkonen JPdoi
10.1007/s00299-005-0936-xkeywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
280-8issue
5eissn
0721-7714issn
1432-203Xjournal_volume
24pub_type
杂志文章abstract::The voltage-dependent anion channels (VDACs), mitochondrial outer membrane components, are present in organisms from fungi to animals and plants. They are thought to function in the regulation of metabolite transport between mitochondria and the cytoplasm. Sufficient knowledge on plant VDACs has been accumulated, so t...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-009-0741-z
更新日期:2009-09-01 00:00:00
abstract::Spermidine and ornithine given to normal auxin-requiring cell suspensions of sugarbeet inhibited peroxidase secretion in the absence of Ca(2+). Habituated (organogenic or not) cells did not respond. Both compounds counteracted the Ca(2+) - promoted enzyme secretion by three cell lines. Auxins (2,4-D and BSAA) did not ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00571295
更新日期:1985-05-01 00:00:00
abstract::Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. In plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and increased number of leaves during the vegetative period. However, the roles of Med8 in other flowering plants are still un...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1118-7
更新日期:2011-11-01 00:00:00
abstract:KEY MESSAGE:Our studies indicate a potential important elicitor candidate which can aid in the fight against a worldwide disease, rice blast. In this study, we report the purification, identification, characterization, and gene cloning of a novel hypersensitive response-inducing protein elicitor (MoHrip2) secreted from...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1663-y
更新日期:2014-11-01 00:00:00
abstract::Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, sug...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232465
更新日期:1996-04-01 00:00:00
abstract::A method has been devised which allows the rapid production of fine suspension cultures of small aggregate size from suspension cultures of large average aggregate size, such as those of Capsicum frutescens. The method, which uses a Waring blender for aseptic homogenisation of cultures, has also been shown to be effec...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269538
更新日期:1988-10-01 00:00:00
abstract::Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-019-02446-6
更新日期:2019-12-01 00:00:00
abstract::Two clones of Populus nigra L. were tested in vivo and in vitro for their susceptibility to three different Agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. Strain C58 proved to be the most virulent.Various parameters affecting Agrobacterium-mediated transformation of P. ni...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233315
更新日期:1994-02-01 00:00:00
abstract:KEY MESSAGE:Phosphate starvation increased the production of phenolic acids by inducing the key enzyme genes in a positive feedback pathway in Saliva miltiorrhiza hairy roots. SPX may be involved in this process. Salvia miltiorrhiza is a wildly popular traditional Chinese medicine used for the treatment of coronary hea...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2007-x
更新日期:2016-09-01 00:00:00
abstract:UNLABELLED:Calmodulin-binding proteins (CBPs) have been known to be involved in both biotic and abiotic stress responses. Recently, two closely related CBPs, Arabidopsis SAR Deficient 1 and CBP60g, were found to belong to a new family of transcription factors that regulate salicylic acid (SA) biosynthesis triggered by ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1247-7
更新日期:2012-07-01 00:00:00
abstract::To improve turfgrasses using genetic engineering, we have developed a transformation system in turf-type tall fescue, one of the most important turfgrass species. Embryogenic cell cultures were established after callus induction from embryos of mature seed. The agarose-bead method with nurse cells was used to culture ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00236381
更新日期:1992-11-01 00:00:00
abstract::Chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells....
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233152
更新日期:1996-08-01 00:00:00
abstract::The expression of green fluorescent protein (GFP) and its inheritance were studied in transgenic oat ( Avena sativa L.) plants transformed with a synthetic green fluorescent protein gene [sgfp(S65T)] driven by a rice actin promoter. In vitro shoot meristematic cultures (SMCs) induced from shoot apices of germinating m...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-002-0542-0
更新日期:2003-01-01 00:00:00
abstract::Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193724
更新日期:1995-12-01 00:00:00
abstract:KEY MESSAGE:The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Despite our knowledge of their advantages, plant-based vaccines remain unavailable ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1911-9
更新日期:2016-03-01 00:00:00
abstract::We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants wa...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050405
更新日期:1998-03-01 00:00:00
abstract:KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1702-8
更新日期:2015-02-01 00:00:00
abstract::Protoplasts isolated from cultured rice cells of an A-58 cytoplasmic male sterile line (A-58 MS)(Oryza sativa L.) were used to investigate the regeneration of rice plants. A cultured cell line (T3) of A-58 MS with a high growth rate and dense cytoplasm was selected. About 10% of the protoplasts prepared from this esta...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269240
更新日期:1986-04-01 00:00:00
abstract::The effects of NaCl, feeder cells and the embedding of protoplasts in calcium alginate have been investigated in an attempt to improve culture conditions of recalcitrant sugar beet (Beta vulgaris L.) mesophyll protoplasts. While the use of NaCl in all instances proved detrimental to protoplast development, the other t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237431
更新日期:1993-04-01 00:00:00
abstract::Leaf explants ofKalanchoe laciniata were cocultivated for different days (2, 4, 6 and 8 days) with disarmedAgrobacterium tumefaciens strains A208SE, GV3111SE and EHA101 carring a binary vector pROA93. The vector contains a cauliflower mosaic virus 35S promotor which drives the coding sequence of neomycin phosphotransf...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00716668
更新日期:1989-06-01 00:00:00
abstract::A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedli...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272760
更新日期:1988-12-01 00:00:00
abstract::We previously reported that Arabidopsis indole-3-acetic acid (IAA)-methyltransferase-1 (IAMT1) catalyzes the conversion of IAA, an essential phytohormone, to methyl-IAA (MeIAA) and that IAMT1 plays an important role in leaf development. Here, we present the possible mechanisms of action of MeIAA in Arabidopsis. We sho...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0458-9
更新日期:2008-03-01 00:00:00
abstract::Anthocyanin is the primary pigment contributing to red, violet, and blue flower color formation. The solubility of anthocyanins is enhanced by UDP glucose: flavonoid 3-O-glucosyltransferase (UFGT) through transfer of the glucosyl moiety from UDP-glucose to 3-hydroxyl group to produce the first stable pigments. To asse...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1006-1
更新日期:2011-06-01 00:00:00
abstract::The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0664-0
更新日期:2009-04-01 00:00:00
abstract::Quantitative real-time polymerase chain reaction (PCR) assays were designed that enabled the zygosity of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) to be determined. The two zygosity assays, based on TaqMan technology that uses a fluorogenic probe which hybridizes to a PCR target...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990100326
更新日期:2001-07-01 00:00:00
abstract::Exposure of Arabidopsis thaliana L. to an acclimation temperature (+4°C) results in a rapid increase of frost tolerance from -3°C to -7°C. This increase could be correlated to changes in soluble protein pattern. Analysis of in vitro translation products from isolated mRNA suggests that induction acts at the transcript...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272740
更新日期:1988-12-01 00:00:00
abstract::Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0571-3
更新日期:2003-06-01 00:00:00
abstract::An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1583-x
更新日期:2014-06-01 00:00:00
abstract:KEY MESSAGE:78 HD-Zip family genes in Phyllostachys edulis were analyzed. Overexpression of Phehdz1 can improve the drought tolerance of transgenic rice and affect its secondary metabolism. Many studies suggested homeodomain-leucine zipper (HD-Zip) transcription factors are important regulators of plant growth and deve...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02625-w
更新日期:2021-01-01 00:00:00
abstract::Extracts of cranberry, rich in flavonols and proanthocyanins, inactivated β-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing the gusA gene. Histochemical GUS assays produced ran...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050294
更新日期:1997-06-01 00:00:00