Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrification and encapsulation-dehydration.

Abstract:

:The first efficient cryopreservation procedure for in vitro-grown shoot tips of raspberry (Rubus idaeus L.) has been developed based on encapsulation-vitrification (EnVi) and encapsulation-dehydration (EnDe). EnVi resulted in higher survival (85%) and regrowth (75%) of cryopreserved shoot tips than EnDe (65 and 50%, respectively). In both cryogenic procedures, shoots regenerated from cryopreserved shoot tips without intermediary callus formation. Histological studies showed that a much larger number of meristematic cells survived following EnVi than EnDe. The EnVi procedure was applied to seven raspberry genotypes with an average survival and regrowth of 71 and 68%, respectively. Regenerated plants showed normal morphology. Results here indicate EnVi as a simple and efficient method for long-term preservation of R. idaeus germplasm.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Wang Q,Laamanen J,Uosukainen M,Valkonen JP

doi

10.1007/s00299-005-0936-x

keywords:

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

280-8

issue

5

eissn

0721-7714

issn

1432-203X

journal_volume

24

pub_type

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