Expression of green fluorescent protein and its inheritance in transgenic oat plants generated from shoot meristematic cultures.

Abstract:

:The expression of green fluorescent protein (GFP) and its inheritance were studied in transgenic oat ( Avena sativa L.) plants transformed with a synthetic green fluorescent protein gene [sgfp(S65T)] driven by a rice actin promoter. In vitro shoot meristematic cultures (SMCs) induced from shoot apices of germinating mature seeds of a commercial oat cultivar, Garry, were used as a transformation target. Proliferating SMCs were bombarded with a mixture of plasmids containing the sgfp(S65T) gene and one of three selectable marker genes, phosphinothricin acetyltransferase (bar), hygromycin phosphotransferase (hpt) and neomycin phosphotransferase (nptII). Cultures were selected with bialaphos, hygromycin B and geneticin (G418), respectively, to identify transgenic tissues. From 289 individual explants bombarded with the sgfp(S65T) gene and one of the three selectable marker genes, 23 independent transgenic events were obtained, giving a 8.0% transformation frequency. All 23 transgenic events were regenerable, and 64% produced fertile plants. Strong GFP expression driven by the rice actin promoter was observed in a variety of tissues of the T(0) plants and their progeny in 13 out of 23 independent transgenic lines. Stable GFP expression was observed in T(2) progeny from five independent GFP-expressing lines tested, and homozygous plants from two lines were obtained. Transgene silencing was observed in T(0) plants and their progeny of some transgenic lines.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Cho MJ,Choi HW,Okamoto D,Zhang S,Lemaux PG

doi

10.1007/s00299-002-0542-0

keywords:

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

467-74

issue

5

eissn

0721-7714

issn

1432-203X

journal_volume

21

pub_type

杂志文章
  • Fertile Indica rice plants regenerated from protoplasts isolated from microspore derived cell suspensions.

    abstract::Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232295

    authors: Datta SK,Datta K,Potrykus I

    更新日期:1990-09-01 00:00:00

  • Production of aposporous gametophytes and calli from Pteris vittata L. pinnae strips cultured in vitro.

    abstract::Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232608

    authors: Kwa SH,Wee YC,Loh CS

    更新日期:1991-10-01 00:00:00

  • The rice MAPKK-MAPK interactome: the biological significance of MAPK components in hormone signal transduction.

    abstract::Mitogen-activated protein kinase (MAPK) signaling cascades are evolutionarily conserved fundamental signal transduction pathways. A MAPK cascade consists of many distinct MAPKKK-MAPKK-MAPK modules linked to various upstream receptors and downstream targets through sequential phosphorylation and activation of the casca...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-013-1437-y

    authors: Singh R,Jwa NS

    更新日期:2013-06-01 00:00:00

  • Phenylalanine ammonia-lyase, phenolic acids and ethylene in alfalfa (Medicago sativa L.) cell cultures in relation to their embryogenic ability.

    abstract::Phenylalanine ammonia-lyase (PAL) activity, contents of phenolic acids and ethylene production during the lag-phase, and contents of phenolic acids at the late exponential phase, showed significant differences in embryogenic (EC) and non-embryogenic (NEC) suspension cultures of Medicago sativa L. Maximum PAL activity ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232569

    authors: Cvikrová M,Meravý L,Macháčková I,Eder J

    更新日期:1991-08-01 00:00:00

  • Genotype-specific heat shock proteins in two maize inbreds.

    abstract::Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233096

    authors: Jorgensen JA,Weng J,Ho TH,Nguyen HT

    更新日期:1992-10-01 00:00:00

  • Progress of tissue culture and genetic transformation research in pigeon pea [Cajanus cajan (L.) Millsp.].

    abstract::Pigeon pea [Cajanus cajan (L.) Millsp.] (Family: Fabaceae) is an important legume crop cultivated across 50 countries in Asia, Africa, and the Americas; and ranks fifth in area among pulses after soybean, common bean, peanut, and chickpea. It is consumed as a major source of protein (21%) to the human population in ma...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-010-0899-4

    authors: Krishna G,Reddy PS,Ramteke PW,Bhattacharya PS

    更新日期:2010-10-01 00:00:00

  • Chromosome elimination and mutation in tetraploid somatic hybrids ofSolanum tuberosum andSolanum phureja.

    abstract::The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00716844

    authors: Pijnacker LP,Ferwerda MA,Puite KJ,Schaart JG

    更新日期:1989-02-01 00:00:00

  • Functional characterization of a stilbene synthase gene using a transient expression system in planta.

    abstract::The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0664-0

    authors: Condori J,Medrano G,Sivakumar G,Nair V,Cramer C,Medina-Bolivar F

    更新日期:2009-04-01 00:00:00

  • Stable transformation of papaya via microprojectile bombardment.

    abstract::Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles car...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232177

    authors: Fitch MM,Manshardt RM,Gonsalves D,Slightom JL,Sanford JC

    更新日期:1990-08-01 00:00:00

  • Organogenesis of stem and leaf protoplasts of a haploid golden delicious apple clone (Malus Xdomestica Borkh.).

    abstract::Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Fo...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00241946

    authors: Patat-Ochatt EM,Boccon-Gibod J,Duron M,Ochatt SJ

    更新日期:1993-01-01 00:00:00

  • Transformation of Actinidia eriantha: a potential species for functional genomics studies in Actinidia.

    abstract::Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on l...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0080-7

    authors: Wang T,Ran Y,Atkinson RG,Gleave AP,Cohen D

    更新日期:2006-05-01 00:00:00

  • Reverse transcriptase domain sequences from Mungbean (Vigna radiata) LTR retrotransposons: sequence characterization and phylogenetic analysis.

    abstract::The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were amplified from mungbean (Vigna radiata) genome using degenerate primers, cloned and sequenced. Among these 34% and 65% of respective clones of copia and gypsy RT sequences pos...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0008-2

    authors: Dixit A,Ma KH,Yu JW,Cho EG,Park YJ

    更新日期:2006-03-01 00:00:00

  • Cell reabsorption in the Euphorbia dulcis endosperm.

    abstract::Euphorbia dulcis endosperm is the site of controlled long lasting endocellular lysis involving segregation and autophagy of portions of the cytoplasm within the endoplasmic reticulum membrane. The lysis products may constitute a food source for the benefit of the early developing embryo. ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231924

    authors: Muccifora S,Gori P

    更新日期:1996-05-01 00:00:00

  • CRISPR/Cas9 mutations in the rice Waxy/GBSSI gene induce allele-specific and zygosity-dependent feedback effects on endosperm starch biosynthesis.

    abstract:KEY MESSAGE:Induced mutations in the waxy locus in rice endosperm did not abolish GBSS activity completely. Compensatory mechanisms in endosperm and leaves caused a major reprogramming of the starch biosynthetic machinery. The mutation of genes in the starch biosynthesis pathway has a profound effect on starch quality ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-019-02388-z

    authors: Pérez L,Soto E,Farré G,Juanos J,Villorbina G,Bassie L,Medina V,Serrato AJ,Sahrawy M,Rojas JA,Romagosa I,Muñoz P,Zhu C,Christou P

    更新日期:2019-03-01 00:00:00

  • Comparative analysis of expressed sequence tags (ESTs) of ginseng leaf.

    abstract::The expressed sequence tags (ESTs) referenced in this report are the first transcriptomes in a leaf from a half-shade ginseng plant. A cDNA library was constructed from samples of the leaves of 4-year-old Panax ginseng plants, which were cultured in a field. The 2,896 P. ginseng cDNA clones represent 1,576 unique sequ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0095-0

    authors: Kim MK,Lee BS,In JG,Sun H,Yoon JH,Yang DC

    更新日期:2006-06-01 00:00:00

  • Bacillus thuringiensis protein production, signal transduction, and insect control in chemically inducible PR-1a/cry1Ab broccoli plants.

    abstract::In an effort to develop a chemically inducible system for insect management, we studied production of Cry1Ab Bacillus thuringiensis (Bt) protein and control of the diamondback moth (DBM), Plutella xylostella L., in inducer-treated and untreated tissues of a broccoli line transformed with a PR-1a/cry1Ab expression cass...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0091-4

    authors: Cao J,Bates SL,Zhao JZ,Shelton AM,Earle ED

    更新日期:2006-06-01 00:00:00

  • Heterologous expression of Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) enhanced chilling tolerance in transgenic eggplant (Solanum melongena L.).

    abstract:KEY MESSAGE:Our study shows that the expression of AtCBF3 and AtCOR15A improved the chilling tolerance in transgenic eggplant. In an attempt to improve chilling tolerance of eggplant (Solanum melongena L) plants, Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) genes both driven by an Ar...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1670-z

    authors: Wan F,Pan Y,Li J,Chen X,Pan Y,Wang Y,Tian S,Zhang X

    更新日期:2014-12-01 00:00:00

  • Down-regulation of MANNANASE7 gene in Brassica napus L. enhances silique dehiscence-resistance.

    abstract:KEY MESSAGE:MANNANASE7 gene in Brassica napus L. encodes a hemicellulose which located at cell wall or extracellular space and dehiscence-resistance can be manipulated by altering the expression of MANNANASE7. Silique dehiscence is an important physiological process in plant reproductive development, but causes heavy y...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02638-5

    authors: Li YL,Yu YK,Zhu KM,Ding LN,Wang Z,Yang YH,Cao J,Xu LZ,Li YM,Tan XL

    更新日期:2021-02-01 00:00:00

  • Hairy roots of Datura candida×D. aurea: effect of culture medium composition on growth and alkaloid biosynthesis.

    abstract::A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050415

    authors: Nussbaumer P,Kapétanidis I,Christen P

    更新日期:1998-03-01 00:00:00

  • Expression of seven carbonic anhydrases in red alga Gracilariopsis chorda and their subcellular localization in a heterologous system, Arabidopsis thaliana.

    abstract:KEY MESSAGE:Red alga, Gracilariopsis chorda, contains seven carbonic anhydrases that can be grouped into α-, β- and γ-classes. Carbonic anhydrases (CAHs) are metalloenzymes that catalyze the reversible hydration of CO2. These enzymes are present in all living organisms and play roles in various cellular processes, incl...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-018-2356-8

    authors: Razzak MA,Lee J,Lee DW,Kim JH,Yoon HS,Hwang I

    更新日期:2019-02-01 00:00:00

  • Genes up-regulated during red coloration in UV-B irradiated lettuce leaves.

    abstract::Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a particularly high abundance of fl...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-006-0255-x

    authors: Park JS,Choung MG,Kim JB,Hahn BS,Kim JB,Bae SC,Roh KH,Kim YH,Cheon CI,Sung MK,Cho KJ

    更新日期:2007-04-01 00:00:00

  • Production of cucumber fruits from the culture of 'Marketmore-76' plantlets.

    abstract::A procedure to produce fruits from cultured shoot tips of Cucumis sativus L. cultivar 'Marketmore-76' in vitro is described. Four-week-old shoot tips, derived from sterile germinated seedlings on a MS medium, were cultured in a 3.8-1 Mason jar using an automated plant culture system. Tips readily generated roots, leav...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232312

    authors: Tisserat B,Galletta PD

    更新日期:1993-11-01 00:00:00

  • AtHSP17.8 overexpression in transgenic lettuce gives rise to dehydration and salt stress resistance phenotypes through modulation of ABA-mediated signaling.

    abstract:KEY MESSAGE:Transgenic Arabidopsis and lettuce plants overexpressing AtHSP17.8 showed ABA-hypersensitive but abiotic stress-resistant phenotypes. ABA treatment caused a dramatic induction of early ABA-responsive genes in AtHSP17.8 -overexpressing transgenic lettuce. Plant small heat shock proteins function as chaperone...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-013-1506-2

    authors: Kim DH,Xu ZY,Hwang I

    更新日期:2013-12-01 00:00:00

  • Development of a cyclic somatic embryogenesis regeneration system for leek (Allium ampeloprasum L.) using zygotic embryos.

    abstract::In leek (Allium ampeloprasum L.) a cyclic system of somatic embryogenesis was developed. Somatic embryos used for cyclic embryogenesis were able to develop the same type of embryogenic callus as zygotic embryos in the primary cycle. For the first time a comparison of the efficiencies of both expiants was made. Ten fam...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233638

    authors: Schavemaker CM,Jacobsen E

    更新日期:1995-01-01 00:00:00

  • RNAseq analysis of cassava reveals similar plant responses upon infection with pathogenic and non-pathogenic strains of Xanthomonas axonopodis pv. manihotis.

    abstract:KEY MESSAGE:An RNAseq-based analysis of the cassava plants inoculated with Xam allowed the identification of transcriptional upregulation of genes involved in jasmonate metabolism, phenylpropanoid biosynthesis and putative targets for a TALE. Cassava bacterial blight, a disease caused by the gram-negative bacterium Xan...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1667-7

    authors: Muñoz-Bodnar A,Perez-Quintero AL,Gomez-Cano F,Gil J,Michelmore R,Bernal A,Szurek B,Lopez C

    更新日期:2014-11-01 00:00:00

  • Production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker.

    abstract::The use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. MAT (multi-auto-transformation) vector system has been one of the different st...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-010-0974-x

    authors: Khan RS,Ntui VO,Chin DP,Nakamura I,Mii M

    更新日期:2011-04-01 00:00:00

  • T-DNA length variability in mannopine hairy root: more than 50 kilobasepairs of pRi T-DNA can integrate in plant cells.

    abstract::Independent carrot (Daucus carota) hairy root lines were established by inoculation of discs taken from the same carrot with Agrobacterium rhizogenes 8196 and A. tumefaciens C58C1(pRi8196) carrying pRi8196. Several lines were compared with respect to T-DNA length. One of them was found to have integrated sequences cov...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00270112

    authors: David C,Petit A,Tempé J

    更新日期:1988-03-01 00:00:00

  • Agrobacterium tumefaciens-mediated transformation of taro (Colocasia esculenta (L.) Schott) with a rice chitinase gene for improved tolerance to a fungal pathogen Sclerotium rolfsii.

    abstract::Taro (Colocasia esculenta) is one of the most important crops in the Pacific Islands, however, taro yields have been declining in Hawaii over the past 30 years partly due to diseases caused by oomycete and fungal pathogens. In this study, an efficient Agrobacterium tumefaciens-mediated transformation method for taro i...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0519-8

    authors: He X,Miyasaka SC,Fitch MM,Moore PH,Zhu YJ

    更新日期:2008-05-01 00:00:00

  • Influence of mitochondria on gene expression in a citrus cybrid.

    abstract::The production of cybrids, combining nucleus of a species with alien cytoplasmic organelles, is a valuable method used for improvement of various crops. Several citrus cybrids have been created by somatic hybridization. These genotypes are interesting models to analyze the impact of cytoplasmic genome change on nuclea...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1014-1

    authors: Bassene JB,Froelicher Y,Navarro L,Ollitrault P,Ancillo G

    更新日期:2011-06-01 00:00:00

  • Expression of zein in long term cultures of wildtype and opaque-2 maize endosperms.

    abstract::It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272049

    authors: Manzocchi LA,Bianchi MW,Viotti A

    更新日期:1989-03-01 00:00:00