Abstract:
:Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus developed from the cut ends of 12.5% of the hypocotyl segments cultured on medium supplemented with 10(-6)M BAP in combination with 10(-6)M 2,4-D or 10(-6)M NAA. Profuse rooting occurred from the hypocotyl explants on medium supplemented with 10(-6)M BAP + 10(-6)M NAA. Incorporation of casein hydrolysate in B5 medium along with 10(-6)M BAP + 10(-7)M 2,4-D enhanced the frequency of cultures with adventitious shoots upto 68.0%. Glutamine, glutamic acid or proline could partially substitute for the effect of casein hydrolysate. Amongst the hypocotyls from 3-14 d old seedlings, the best caulogenesis was obtained with hypocotyls from 7 d old seedlings both in presence or absence of casein hydrolysate. Best rooting of shoots was achieved on half-strength B5 medium supplemented with 10(-6)M IBA. After hardening, plantlets were successfully transferred to the soil.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Rajasubramaniam S,Pardha Saradhi Pdoi
10.1007/BF00232932subject
Has Abstractpub_date
1994-08-01 00:00:00pages
619-22issue
11eissn
0721-7714issn
1432-203Xjournal_volume
13pub_type
杂志文章abstract::An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...
journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1995-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232527
更新日期:1992-05-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1993-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1994-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231624
更新日期:1994-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00299-020-02638-5
更新日期:2021-02-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00299-013-1422-5
更新日期:2013-08-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272780
更新日期:1987-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233638
更新日期:1995-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-03-01 00:00:00
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更新日期:1991-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1995-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269636
更新日期:1986-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232092
更新日期:1990-07-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0854-4
更新日期:2010-07-01 00:00:00
abstract:KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2217-x
更新日期:2018-01-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232858
更新日期:1990-10-01 00:00:00