Protoplast-derived streptomycin resistant plants of the forage legume Onobrychis viciifolia Scop. (sainfoin).

Abstract:

:Approximately 10(6) protoplast-derived cell colonies of sainfoin were stressed with streptomycin and two resistant colonies were recovered. Plants regenerated from these colonies could be recallused on streptomycin-containing medium three years after growth in the absence of the antibiotic.Ultrastructural studies showed cells of resistant callus grown in the presence of streptomycin to contain chloroplasts with internal thykaloids and grana. Such mutant plants should be useful in designing biochemical selection schemes to recover somatic hybrids and cybrids.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Hamill JD,Ahuja PS,Davey MR,Cocking EC

doi

10.1007/BF00269636

subject

Has Abstract

pub_date

1986-12-01 00:00:00

pages

439-41

issue

6

eissn

0721-7714

issn

1432-203X

journal_volume

5

pub_type

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