Direct somatic embryogenesis and plant regeneration from protoplasts of Bupleurum scorzonerifolium Willd.

Abstract:

:Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditions favorable to direct embryo formation were investigated, and the nature of the callus used for protoplast preparation was found to be a critical factor. The osmotic concentration and the composition of the culture medium including the phytohormone combinations were also important.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Guang-Min X,Zhongyi L,Guang-Qin G,Hui-Min C

doi

10.1007/BF00232170

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

155-8

issue

3

eissn

0721-7714

issn

1432-203X

journal_volume

11

pub_type

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