Abstract:
KEY MESSAGE:This study established an efficient method of regenerating plants of Ficus lyrata and producing purple-leaved F. lyrata plants through genetic transformation using a VvMybA1 gene of grapevine. ABSTRACT:Ficus lyrata, a species with unique violin- or guitar-shaped leaves, was regenerated from leaf-derived calli cultured on Murashige and Skoog (MS) basal medium supplemented with 4.5 μM N-phenyl-N'-1, 2, 3-thiadiazol-5-yl urea (TDZ) and 0.5 μM α-naphthalene acetic acid (NAA). Leaf discs were inoculated with Agrobacterium tumefaciens strain EHA 105 harboring a binary vector DEAT that contains the VvMybA1 gene and neomycin phosphotransferase (npt II) gene and subsequently cultured on the established regeneration medium supplemented with 100 mg l(-1) kanamycin. Results showed that 87.5 % of the leaf discs produced kanamycin-resistant callus, and 68.8 % of them produced adventitious shoots. Transgenic plants with three leaf colors including green, green-purple, and purple were produced. Regular and quantitative real-time PCR analyses confirmed the integration of transgenes into the host genome. Semi-quantitative RT-PCR analysis indicated that the VvMybA1 gene was responsible for the purple-colored phenotype. Purple-leaved plants with strong color stability grew vigorously in a greenhouse. This study illustrated the feasibility of using a genetically engineered VvMybA1 gene for drastic modification of leaf color of an important woody ornamental plant.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Zhao J,Li ZT,Chen J,Henny RJ,Gray DJ,Chen Jdoi
10.1007/s00299-013-1491-5subject
Has Abstractpub_date
2013-11-01 00:00:00pages
1783-93issue
11eissn
0721-7714issn
1432-203Xjournal_volume
32pub_type
杂志文章abstract:UNLABELLED:Calmodulin-binding proteins (CBPs) have been known to be involved in both biotic and abiotic stress responses. Recently, two closely related CBPs, Arabidopsis SAR Deficient 1 and CBP60g, were found to belong to a new family of transcription factors that regulate salicylic acid (SA) biosynthesis triggered by ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1247-7
更新日期:2012-07-01 00:00:00
abstract::A restricted part of the undeveloped stem of the garlic clove, called the "stem disc", which is just under the basement of the immature foliage leaves, proved to be a very potent explant for the micropropagation of garlic. Twenty to thirty tissue-cultured shoots consistently were differentiated from a single clove dur...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050481
更新日期:1998-07-01 00:00:00
abstract::We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants wa...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050405
更新日期:1998-03-01 00:00:00
abstract::A procedure has been developed for the clonal propagation of Picrorhiza kurroa Royle ex Benth. through shoot tip culture. Murashige and Skoog's medium (1962) supplemented with kinetin (3.0 to 5.0 mg/l) supported rapid proliferation of multiple shoots from the explants. Addition of indole-3-acetic acid (1.0 mg/l) to th...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269324
更新日期:1988-05-01 00:00:00
abstract::A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231799
更新日期:1991-03-01 00:00:00
abstract::Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootsto...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0422-8
更新日期:2008-01-01 00:00:00
abstract::Suspension cultured cells of the moss, Barbula unguiculata, grow actively in both light and dark culture. Light-grown cells contain chlorophyll and exhibit an undifferentiated callus form. When cells are transferred to a dark condition, they develop into protonemata. Protonemata formation in the dark can be inhibited ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272039
更新日期:1989-03-01 00:00:00
abstract::Protoplasts isolated from embryogenic callus cultures derived from immature embryos ofZea mays L. are suitable for analysis of transient gene expression using electroporation-mediated DNA transfer. Expression of introduced genes is comparable to the levels obtained with protoplasts from Black Mexican Sweet suspension ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00716827
更新日期:1989-03-01 00:00:00
abstract::Plum hypocotyl slices were transformed with the coat protein (CP) gene of plum pox virus (PPV-CP) following cocultivation with Agrobacterium tumefaciens containing the plasmid pGA482GG/PPVCP-33. This binary vector carries the PPV-CP gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidas...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233291
更新日期:1994-11-01 00:00:00
abstract::We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233813
更新日期:1991-11-01 00:00:00
abstract::A microscopical study was performed in order to localize chromogenic valepotriates in roots (in vivo) and in callus and root organ cultures (in vitro) of valerian plants. These in vitro cultures (producing valepotriates) contained lipid droplets having the same aspect as those containing essential oil described in the...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270186
更新日期:1983-12-01 00:00:00
abstract::In this opinion article, we briefly review the status of crop biotechnology research-with emphasis on the development of GM crops-in Jamaica and Venezuela. We focus on the transgenic papayas developed for both countries, and examine the factors hindering not only the development and application of this biotechnologica...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0988-4
更新日期:2011-05-01 00:00:00
abstract::An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1583-x
更新日期:2014-06-01 00:00:00
abstract::To study the developmental transition of chloroplasts from C(3) to C(4) photosynthesis in the terrestrial single-cell C(4) species Bienertia sinuspersici, a regeneration protocol was developed. Stem explant material developed callus either with or without red nodular structures (RNS) when cultured on Murashige-Skoog (...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1067-1
更新日期:2011-08-01 00:00:00
abstract::A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0697-z
更新日期:2009-06-01 00:00:00
abstract:KEY MESSAGE:The radish WRKY gene family was genome-widely identified and played critical roles in response to multiple abiotic stresses. The WRKY is among the largest transcription factors (TFs) associated with multiple biological activities for plant survival, including control response mechanisms against abiotic stre...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2190-4
更新日期:2017-11-01 00:00:00
abstract:KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2217-x
更新日期:2018-01-01 00:00:00
abstract::A reliable protocol for the transformation of cultivated sunflower (Helianthus annuus L.) has been established, based on microprojectile bombardment of half shoot apices in combination with Agrobacterium tumefaciens coculture. Transgenic shoots have been obtained from 5 inbred lines, although transformation efficienci...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233766
更新日期:1994-12-01 00:00:00
abstract::The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcohol dehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intronl along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli β-glu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00236096
更新日期:1993-07-01 00:00:00
abstract::The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-(13)C] proline was followed by (13)C NMR and ninhydrin analyses of suspensions cultured...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269924
更新日期:1988-08-01 00:00:00
abstract::Donor plants of Hordeum vulgare L. cv. Igri were grown in a conditioned environment to minimise fluctuations in the composition of the microspore population. After isolation different types of microspores were identified within each population, amongst others an embryogenic subpopulation. It was shown that the optimum...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233415
更新日期:1993-10-01 00:00:00
abstract::The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1183-y
更新日期:2012-04-01 00:00:00
abstract::Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were fou...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269896
更新日期:1985-12-01 00:00:00
abstract::Cell structures were compared between alkaloid-producing and non-producing cell cultures of Coptis japonica and Thalictrum minus by electron microscopic observation. In alkaloid-producing cells of C. japonica, prior to the onset of alkaloid synthesis, the vacuoles showed a greater volume than in non-producing cells. T...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269721
更新日期:1986-02-01 00:00:00
abstract:: Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the us...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050014
更新日期:2000-01-01 00:00:00
abstract::A protocol was developed for biolistic transformation of hybrid bermudagrass cv. TifEagle using the bar gene. TifEagle is an ultradwarf used exclusively on golf greens. Herbicide resistance should serve as a useful management tool, especially if methyl-bromide is unavailable for fumigation prior to plant establishment...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0736-0
更新日期:2004-03-01 00:00:00
abstract::Diuraphis noxia (Russian wheat aphid, RWA) is a major pest on wheat in South Africa and most other wheat growing countries. Being a probing-sucking insect, RWAs insert their stylets into the phloem sieve elements and feed on the phloem sap. This feeding causes necrotic lesions in resistant varieties, or decoloration o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0001-9
更新日期:2006-02-01 00:00:00
abstract::LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000237
更新日期:2000-11-01 00:00:00
abstract::Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). How...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269060
更新日期:1989-12-01 00:00:00
abstract::Compact, friable and embryogenic calli were initiated from immature inflorescences and young leaf bases of one week old seedlings of Paspalum scrobiculatum cultured on MS medium supplemented with 2,4-D. A stable, embryogenic suspension culture was initiated from these calli and maintained in a liquid version of the sa...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00274134
更新日期:1989-05-01 00:00:00