Abstract：
:RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV) CP (coat protein) gene were amplified by RT-PCR, and cloned into the inverted-repeat cloning vector pUCCRNAi. The two recombinant plasmids were transformed individually into E. coli HT115, an RNase-III deficient strain, and dsRNA was induced by isopropyl-β-D: -thiogalactopyranoside (IPTG). The crude extracts of E. coli HT115 containing large amounts of dsRNA were applied to plants as a spray and the experiment confirmed a preventative efficacy. Our findings demonstrated that spraying crude dsRNA-containing extracts inhibited SCMV infection, and the dsRNA derived from an upstream region (CP1) was more effective than was dsRNA derived from a downstream region (CP2) of the SCMV CP gene. The results provide a valuable tool for plant viral control using dsRNA and the PTGS approach.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Gan D,Zhang J,Jiang H,Jiang T,Zhu S,Cheng Bdoi
10.1007/s00299-010-0911-zsubject
Has Abstractpub_date
2010-11-01 00:00:00pages
1261-8issue
11eissn
0721-7714issn
1432-203Xjournal_volume
29pub_type
杂志文章abstract：KEY MESSAGE:Rice and chickpea GDPD s are transcriptionally influenced by mineral deficiencies; especially, by phosphate starvation and CaGDP1 encodes an active glycerophosphodiester phosphodiesterase enzyme. Glycerophosphodiester phosphodiesterases (GDPDs) are enzymes involved in the degradation of glycerophosphodieste...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1984-0
更新日期：2016-08-01 00:00:00
abstract：:Salt tolerant cell lines have been selected from Medicago sativa, by a single step selection process on tissue culture medium containing 1% NaCl. Plants regenerated from these lines show improved salt tolerance compared to parent plants. The regenerated plants are vigorous, have flowered and are self fertile. The cell...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232511
更新日期：1991-12-01 00:00:00
abstract：:A simple and reliable method was developed for isolating pollen nuclei from Brassica napus and Triticum aestivum for DNA analysis using flow cytometry. The nuclei were released from pollen by ultrasonic treatment. The isolated nuclei following filtration through nylon mesh and a purification procedure were suitable fo...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0830-y
更新日期：2004-10-01 00:00:00
abstract：:Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Reg...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272790
更新日期：1987-12-01 00:00:00
abstract：: Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-old nodular green ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050763
更新日期：2000-04-01 00:00:00
abstract：:In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00193714
更新日期：1995-12-01 00:00:00
abstract：:Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide reduction through a plasma membrane redox system which may be associated with proton translocation. Evidence shows that endogenous pyridine nucleotides serve as hydrogen donors for the reaction. The proton translocating function of the redox system ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269886
更新日期：1985-12-01 00:00:00
abstract：:Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 microM of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0092-3
更新日期：2006-04-01 00:00:00
abstract：KEY MESSAGE:The Arabidopsis AP2/ERF family transcription factor AtERF15 is nuclear-localized and positively regulates ABA and stress responses. Abscisic acid (ABA) is a major plant hormone that controls the expression of hundreds genes involved in various aspects of plant growth and development, such as seed developmen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1688-2
更新日期：2015-01-01 00:00:00
abstract：:KEY MESSAGE : Two soybean cultivars showed markedly different drought tolerance. G6PDH plays a central role in the process of H ( 2 ) O ( 2 ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1374-1
更新日期：2013-03-01 00:00:00
abstract：KEY MESSAGE:SnYSL3 encodes a plasma-localized transporter delivering various metal-nicotianamine complexes. The expression of SnYSL3 is up-regulated by excess Cd, suggesting an important role for SnYSL3 in response to Cd stress. The Yellow Stripe-Like (YSL) transporters have been proposed to participate in metal uptake...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2079-7
更新日期：2017-02-01 00:00:00
abstract：:Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and alpha-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0224-4
更新日期：2007-02-01 00:00:00
abstract：:A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050415
更新日期：1998-03-01 00:00:00
abstract：:Eukaryotic gene expression is regulated at least by two processes, RNA interference at the post-transcriptional level and chromatin modification at the transcriptional level. Distinct small RNAs (approximately 21-24 nucleotides; sRNAs) were demonstrated to play vital roles in facilitating gene silencing. In plants, th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1046-6
更新日期：2011-07-01 00:00:00
abstract：:The stability of foreign protein production in genetically engineered plant cells was studied. A cultured tobacco cell line was transformed with a chimeric molecule carrying a bacterial gene, ß-glucuronidase (GUS), under plant regulatory sequences. The specific GUS activity was monitored for 294 days with ten independ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234589
更新日期：1991-12-01 00:00:00
abstract：:A double-gene construct with one chitinase and one beta-1,3-glucanase gene from barley, both driven by enhanced 35S promoters, was transformed into oilseed rape. From six primary transformants expressing both transgenes 10 doubled haploid lines were produced and studied for five generations. The number of inserted cop...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0153-2
更新日期：2006-09-01 00:00:00
abstract：:The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0917-0
更新日期：2005-06-01 00:00:00
abstract：KEY MESSAGE:Overexpression of SoSnRK2.1 improved drought tolerance and growth of tobacco plants. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. In this research, SoSnRK2.1...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2004-0
更新日期：2016-09-01 00:00:00
abstract：:The expressed sequence tags (ESTs) referenced in this report are the first transcriptomes in a leaf from a half-shade ginseng plant. A cDNA library was constructed from samples of the leaves of 4-year-old Panax ginseng plants, which were cultured in a field. The 2,896 P. ginseng cDNA clones represent 1,576 unique sequ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0095-0
更新日期：2006-06-01 00:00:00
abstract：KEY MESSAGE:The recovery of non-functional-enhanced green fluorescence protein can be used as indicator to facilitate the identification of mutants generated by CRISPR/Cas9. The CRISPR/Cas9 system is a powerful tool for genome editing and it has been employed to knock out genes of interest in multiple plant species. Id...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02465-3
更新日期：2019-12-01 00:00:00
abstract：:Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of call...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00239094
更新日期：1993-01-01 00:00:00
abstract：:Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparati...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270237
更新日期：1982-10-01 00:00:00
abstract：:This article describes conditions to optimize the yield of viable protoplasts from callus tissue of Asparagus densiflorus cv. Sprengeri and their subsequent regeneration into plantlets. Callus tissue was initiated by culturing spear sections (5-7 mm) on Murashige and Skoog (MS) medium supplemented with 0.8% (wt/vol) B...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050364
更新日期：1997-12-01 00:00:00
abstract：:The expression of green fluorescent protein (GFP) and its inheritance were studied in transgenic oat ( Avena sativa L.) plants transformed with a synthetic green fluorescent protein gene [sgfp(S65T)] driven by a rice actin promoter. In vitro shoot meristematic cultures (SMCs) induced from shoot apices of germinating m...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-002-0542-0
更新日期：2003-01-01 00:00:00
abstract：:Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233096
更新日期：1992-10-01 00:00:00
abstract：:The growth and sweet diterpene glucosides of Stevia plants propagated by stem-tip cultures were compared with those of the control plants propagated by seeds. There was no significant difference between the two groups both in growth and in chemical composition. As for the contents of sweet diterpene glucosides, howeve...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270194
更新日期：1984-10-01 00:00:00
abstract：KEY MESSAGE:Wheat MAPK gene TaMPK14 is N starvation response and is crucial in modulating plant low-N stress tolerance. Improving plant N use efficiency (NUE) contributes largely to the sustainable crop production worldwide. In this study, TaMPK14, a mitogen-activated protein kinase (MAPK) family gene in T. aestivum, w...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02564-6
更新日期：2020-10-01 00:00:00
abstract：KEY MESSAGE:Drastic changes in soil water content can activate the short-term high expression of key enzyme-encoding genes involved in secondary metabolite synthesis thereby increasing the content of secondary metabolites. Bupleurum chinense DC. is a traditional medicinal herb that is famous for its abundant saikosapon...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02436-8
更新日期：2019-09-01 00:00:00
abstract：:Treatment of Allium cepa L. cellsuspension cultures with a biotic elicitor derived from the fungus Botrytis cinerea, resulted in phytoalexin synthesis. Two phytoalexins, 5-octylcyclopenta-1,3-dione and 5-hexyl-cyclopenta-1,3-dione, were accumulated in cultured onion cells. Removal of extracellular Ca(2+) by the calciu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231593
更新日期：1996-09-01 00:00:00
abstract：KEY MESSAGE:An antigenic protein targeting two epitopes from the Zaire ebolavirus GP1 protein was expressed in plant cells rendering an antigen capable of inducing humoral responses in mouse when administered subcutaneously or orally. The 2014 Ebola outbreak made clear that new treatments and prophylactic strategies to...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2088-6
更新日期：2017-02-01 00:00:00
