Hairy roots of Datura candida×D. aurea: effect of culture medium composition on growth and alkaloid biosynthesis.

abstract：:Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulat...

journal_title：Plant cell reports

authors： Pillai KG,Rao IU,Rao IV,Ram HY

更新日期：1992-01-01 00:00:00

abstract：:The rooting capacities of tips of seedling, juvenile and mature shoots of Sequoiadendron giganteum were compared on different rooting media (inductive and expressive media) after passage on an elongating medium. None of the cuttings rooted when continuously kept on medium containing the auxin NAA and vitamin D2. Perox...

journal_title：Plant cell reports

authors： Berthon JY,Boyer N,Gaspar T

更新日期：1987-10-01 00:00:00

abstract：:The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems,...

journal_title：Plant cell reports

authors： Sivamani E,Shen P,Opalka N,Beachy RN,Fauquet CM

更新日期：1996-01-01 00:00:00

abstract：KEY MESSAGE:Phosphate starvation increased the production of phenolic acids by inducing the key enzyme genes in a positive feedback pathway in Saliva miltiorrhiza hairy roots. SPX may be involved in this process. Salvia miltiorrhiza is a wildly popular traditional Chinese medicine used for the treatment of coronary hea...

journal_title：Plant cell reports

authors： Liu L,Yang D,Liang T,Zhang H,He Z,Liang Z

更新日期：2016-09-01 00:00:00

abstract：KEY MESSAGE:Marker-free transgenic plants can be generated with high efficiency by using the Cre/ lox P self-excision system controlled by the pollen- and embryo-specific Arabidopsis DLL promoter. In this work, we aimed to study the feasibility of using the pollen- and embryo-specific DLL promoter of the At4g16160 gene...

journal_title：Plant cell reports

authors： Polóniová Z,Jopčík M,Matušíková I,Libantová J,Moravčíková J

更新日期：2015-03-01 00:00:00

abstract：:Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or ligh...

journal_title：Plant cell reports

authors： Mizukami H,Tomita K,Ohashi H

更新日期：1989-12-01 00:00:00

abstract：KEY MESSAGE:Galactoglucomannan oligosaccharides seem to interact with auxin in xylogenic cell culture, thus influencing mainly metaxylem-like tracheary element differentiation depending on timing with hormones and the process kinetics. Complex mapping of Zinnia mesophyll cell transdifferentiation into tracheary element...

journal_title：Plant cell reports

authors： Kákošová A,Digonnet C,Goffner D,Lišková D

更新日期：2013-04-01 00:00:00

abstract：:Emission and plant uptake of atmospheric nitrogen oxides (NO + NO(2)) significantly influence regional climate change by regulating the oxidative chemistry of the lower atmosphere, species composition and the recycling of carbon and nutrients, etc. Plant uptake of nitrogen dioxide (NO(2)) is concentration-dependent an...

journal_title：Plant cell reports

authors： Hu Y,Sun G

更新日期：2010-10-01 00:00:00

abstract：:A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...

journal_title：Plant cell reports

authors： Walter C,Grace LJ,Wagner A,White DWR,Walden AR,Donaldson SS,Hinton H,Gardner RC,Smith DR

更新日期：1998-04-01 00:00:00

abstract：:Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibite...

journal_title：Plant cell reports

authors： Minocha R,Minocha SC,Komamine A,Shortle WC

更新日期：1991-06-01 00:00:00

abstract：:Spermidine and ornithine given to normal auxin-requiring cell suspensions of sugarbeet inhibited peroxidase secretion in the absence of Ca(2+). Habituated (organogenic or not) cells did not respond. Both compounds counteracted the Ca(2+) - promoted enzyme secretion by three cell lines. Auxins (2,4-D and BSAA) did not ...

journal_title：Plant cell reports

authors： Kevers C,Gaspar T,Penel C,Greppin H

更新日期：1985-05-01 00:00:00

abstract：: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this met...

journal_title：Plant cell reports

authors： Kamaté K,Rodriguez-Llorente ID,Scholte M,Durand P,Ratet P,Kondorosi E,Kondorosi A,Trinh TH

更新日期：2000-06-01 00:00:00

abstract：:Modern powerful techniques in plant biotechnology have been developed in lilies (Lilium spp., Liliaceae) to propagate, improve and make new phenotypes. Reliable in vitro culture methods are available to multiply lilies rapidly and shorten breeding programs. Lilium is also an ideal model plant to study in vitro pollina...

journal_title：Plant cell reports

authors： Bakhshaie M,Khosravi S,Azadi P,Bagheri H,van Tuyl JM

更新日期：2016-09-01 00:00:00

abstract：:Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...

journal_title：Plant cell reports

authors： Moreno OA,Vazquez-Duhalt R,Nolasco H

更新日期：1990-07-01 00:00:00

abstract：:Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. 'Russet Burbank'. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency o...

journal_title：Plant cell reports

authors： Newell CA,Rozman R,Hinchee MA,Lawson EC,Haley L,Sanders P,Kaniewski W,Tumer NE,Horsch RB,Fraley RT

更新日期：1991-05-01 00:00:00

abstract：:Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). How...

journal_title：Plant cell reports

authors： Ellis JS,Jennings AC,Edwards LA,Mavandad M,Lamb CJ,Dixon RA

更新日期：1989-12-01 00:00:00

abstract：:A method has been devised which allows the rapid production of fine suspension cultures of small aggregate size from suspension cultures of large average aggregate size, such as those of Capsicum frutescens. The method, which uses a Waring blender for aseptic homogenisation of cultures, has also been shown to be effec...

journal_title：Plant cell reports

authors： Williams PD,Wilkinson AK,Lewis JA,Black GM,Mavituna F

更新日期：1988-10-01 00:00:00

abstract：:The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...

journal_title：Plant cell reports

authors： Moriya Y,Yamamoto K,Okada K,Iwanami H,Bessho H,Nakanishi T,Takasaki T

更新日期：2007-03-01 00:00:00

abstract：:Arabinogalactan proteins (AGPs) are important proteoglycans regulating somatic embryogenesis in diverse plant species. Embryogenic cells of somatic embryos are covered by special extracellular cell wall layer called extracellular surface matrix network (ECMSN) at their early developmental stages. Here we show that hig...

journal_title：Plant cell reports

authors： Samaj J,Salaj T,Matúsová R,Salaj J,Takác T,Samajová O,Volkmann D

更新日期：2008-02-01 00:00:00

abstract：:Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...

journal_title：Plant cell reports

authors： Aviv D,Chen R,Galun E

更新日期：1986-06-01 00:00:00

abstract：:Transgenic herbicide tolerant Acacia sinuata plants were produced by transformation with the bar gene conferring phosphinothricin resistance. Precultured hypocotyl explants were infected with Agrobacterium tumefaciens strain EHA105 in the presence of 100 microM acetosyringone and shoots regenerated on MS (Murashige an...

journal_title：Plant cell reports

authors： Vengadesan G,Amutha S,Muruganantham M,Anand RP,Ganapathi A

更新日期：2006-11-01 00:00:00

abstract：:Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus dev...

journal_title：Plant cell reports

authors： Rajasubramaniam S,Pardha Saradhi P

更新日期：1994-08-01 00:00:00

abstract：:The effects of cytokinins on the different branches of the indole alkaloid pathway were investigated in Catharanthus roseus cell cultures. Addition of zeatin to a 2,4-dichlorophenoxyacetic acid-containing medium decreased tryptamine levels and increased the bioconversion of secologanin to ajmalicine. Zeatin also enhan...

journal_title：Plant cell reports

authors： Decendit A,Petit G,Andreu F,Doireau P,Mérillon JM,Rideau M

更新日期：1993-10-01 00:00:00

abstract：:The first efficient cryopreservation procedure for in vitro-grown shoot tips of raspberry (Rubus idaeus L.) has been developed based on encapsulation-vitrification (EnVi) and encapsulation-dehydration (EnDe). EnVi resulted in higher survival (85%) and regrowth (75%) of cryopreserved shoot tips than EnDe (65 and 50%, r...

journal_title：Plant cell reports

authors： Wang Q,Laamanen J,Uosukainen M,Valkonen JP

更新日期：2005-07-01 00:00:00

abstract：:This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that wer...

journal_title：Plant cell reports

authors： Igasaki T,Sato T,Akashi N,Mohri T,Maruyama E,Kinoshita I,Walter C,Shinohara K

更新日期：2003-11-01 00:00:00

abstract：:Programmed cell death (PCD) is a genetically encoded, active process that results in the death of individual cells, tissues, or whole organs, which plays an important role in the life cycles of plants and animals. Previous studies show that methyl salicylate (MeSA) is a defense signal molecular associated with systemi...

journal_title：Plant cell reports

authors： Yun LJ,Chen WL

更新日期：2011-07-01 00:00:00

abstract：:The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...

journal_title：Plant cell reports

authors： Prabu G,Prasad DT

更新日期：2012-04-01 00:00:00

abstract：:The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...

journal_title：Plant cell reports

authors： Roussy I,Dubois F,Sangwan RS,Sangwan-Norreel BS

更新日期：1996-12-01 00:00:00

abstract：:The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of n...

journal_title：Plant cell reports

authors： Khan BM,Dwivedi UN,Rawal SK,Mascarenhas AF

更新日期：1984-08-01 00:00:00

abstract：KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...

journal_title：Plant cell reports

authors： Coronel CJ,González AI,Ruiz ML,Polanco C

更新日期：2018-01-01 00:00:00