Does pretreatment by rhodamine 6-G affect the mitochondrial composition of fusion-derived Nicotiana cybrids?

Abstract:

:Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was analyzed by Southern-blot hybridization. We found that cybrids which resulted from N. rustica (donor) protoplasts, fused with R6G-treated albino protoplasts, had only parental-type mtDNA. When another donor, with N. undulata mtDNA, was used, most of the resulting cybrids contained non-parental mtDNA. Only one cybrid (out of 12) had N. undulata -type (donor) mtDNA.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Aviv D,Chen R,Galun E

doi

10.1007/BF00269126

subject

Has Abstract

pub_date

1986-06-01 00:00:00

pages

227-30

issue

3

eissn

0721-7714

issn

1432-203X

journal_volume

5

pub_type

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