Production of somatic hybrid tissues following chemical and electrical fusion of protoplasts from albino cell suspensions ofMedicago sativa andM. borealis.

abstract：:High yields of protoplasts were obtained from leaves of aseptically grown plants and calli originated from different explants, in several cultivars of Cajanus cajan L. The protoplasts divided to form cell clusters in modified KM 8p medium and developed to protocolonies after dilution with liquid Caboche's medium withi...

journal_title：Plant cell reports

authors： Sarangi BK,Kuchuk N,Gleba YY

更新日期：1992-08-01 00:00:00

abstract：KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...

journal_title：Plant cell reports

authors： Soares EL,Lima MLB,Nascimento JRS,Soares AA,Coutinho ÍAC,Campos FAP

更新日期：2017-11-01 00:00:00

abstract：:A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedli...

journal_title：Plant cell reports

authors： Heberle-Bors E,Charvat B,Thompson D,Schernthaner JP,Barta A,Matzke AJ,Matzke MA

更新日期：1988-12-01 00:00:00

abstract：:Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which β-glucuronidase (GUS) is exp...

journal_title：Plant cell reports

authors： Kokko HI,Kärenlampi SO

更新日期：1998-07-01 00:00:00

abstract：:The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...

journal_title：Plant cell reports

authors： Gu XF,Zhang JR

更新日期：2005-03-01 00:00:00

abstract：:This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...

journal_title：Plant cell reports

authors： Caillot S,Rosiau E,Laplace C,Thomasset B

更新日期：2009-03-01 00:00:00

abstract：KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...

journal_title：Plant cell reports

authors： Snyman SJ,Hajari E,Watt MP,Lu Y,Kridl JC

更新日期：2015-05-01 00:00:00

abstract：:The anthracnose fungus Colletotrichum gloeosporioides deleteriously affects unripe pepper fruit, but not ripe fruit. Here, we show that the induction of local acquired resistance (LAR) by salicylic acid (SA), 2,6-dichloroisonicotinic acid, or benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester pretreatment pro...

journal_title：Plant cell reports

authors： Lee S,Hong JC,Jeon WB,Chung YS,Sung S,Choi D,Joung YH,Oh BJ

更新日期：2009-10-01 00:00:00

abstract：:Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a particularly high abundance of fl...

journal_title：Plant cell reports

authors： Park JS,Choung MG,Kim JB,Hahn BS,Kim JB,Bae SC,Roh KH,Kim YH,Cheon CI,Sung MK,Cho KJ

更新日期：2007-04-01 00:00:00

abstract：:Advancement in the field of genetics and genomics after the discovery of Mendel's laws of inheritance has led to map the genes controlling qualitative and quantitative traits in crop plant species. Mapping of genomic regions controlling the variation of quantitatively inherited traits has become routine after the adve...

journal_title：Plant cell reports

authors： Kumar J,Gupta DS,Gupta S,Dubey S,Gupta P,Kumar S

更新日期：2017-08-01 00:00:00

abstract：:Cell proliferation has been induced from the cambial zone of a branch of Acacia senegal, kept on the basal Knop and Ball medium in the presence of auxin. Transferred on the more complete nutrient medium of Schenk and Hildebrandt, the colonies gave rise to several cell lines. One of the friable lines, consisting of agg...

journal_title：Plant cell reports

authors： Hustache G,Barnoud F,Joseleau JP

更新日期：1986-10-01 00:00:00

abstract：:Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...

journal_title：Plant cell reports

authors： Zhang JJ,Shu QY,Liu ZA,Ren HX,Wang LS,De Keyser E

更新日期：2012-02-01 00:00:00

abstract：:Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A...

journal_title：Plant cell reports

authors： Gao ZM,Li CL,Peng ZH

更新日期：2011-11-01 00:00:00

abstract：:A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protop...

journal_title：Plant cell reports

authors： Edwards ES,Roux SJ

更新日期：1998-06-01 00:00:00

abstract：:In this opinion article, we briefly review the status of crop biotechnology research-with emphasis on the development of GM crops-in Jamaica and Venezuela. We focus on the transgenic papayas developed for both countries, and examine the factors hindering not only the development and application of this biotechnologica...

journal_title：Plant cell reports

authors： Fermin G,Tennant P

更新日期：2011-05-01 00:00:00

abstract：:Optimal protoplast yields from cotyledons (2.0×10(6) protoplasts/ 0.5 g tissue) and from true leaves (5.0×10(6) protoplasts/g tissue) of two Cucumis sativus genotypes were obtained following a 16 h digestion with, respectively, 1.25% pectinase+0.5% Cellulysin and 0.5 % pectinase+ 1.0% Cellulysin. Enzyme solutions were...

journal_title：Plant cell reports

authors： Punja ZK,Tang FA,Sarmento GG

更新日期：1990-07-01 00:00:00

abstract：:Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some c...

journal_title：Plant cell reports

authors： Mullins KV,Llewellyn DJ,Hartney VJ,Strauss S,Dennis ES

更新日期：1997-09-01 00:00:00

abstract：:Stable cell suspension cultures have been established from immature endosperms of A69Y wild-type and opaque-2 maize (Zea mays L.). Cultured cells are capable of storage protein (zein) synthesis and accumulation throughout the growth period. Electrophoretic patterns of zeins show, for opaque-2 cells, the preferential i...

journal_title：Plant cell reports

authors： Manzocchi LA

更新日期：1991-02-01 00:00:00

abstract：:Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes. The transformed cultures of ginseng could be subcultured as an axenic root culture in the absence of phytohormones, and grew with extensive lateral branches more rapidly t...

journal_title：Plant cell reports

authors： Yoshikawa T,Furuya T

更新日期：1987-12-01 00:00:00

abstract：:Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...

journal_title：Plant cell reports

authors： Datta SK,Datta K,Potrykus I

更新日期：1990-09-01 00:00:00

abstract：:The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...

journal_title：Plant cell reports

authors： Liau CH,You SJ,Prasad V,Hsiao HH,Lu JC,Yang NS,Chan MT

更新日期：2003-06-01 00:00:00

abstract：: Embryogenic soybean [Glycine max (L.) Merrill.] suspension cultures were bombarded with five different gene constructions encoding the jellyfish (Aequorea victoria) green fluorescent protein (GFP). These constructions had altered codon usage compared to the native GFP gene and mutations that increased the solubility ...

journal_title：Plant cell reports

authors： Ponappa T,Brzozowski AE,Finer JJ

更新日期：1999-11-01 00:00:00

abstract：:The effects of cytokinins on the different branches of the indole alkaloid pathway were investigated in Catharanthus roseus cell cultures. Addition of zeatin to a 2,4-dichlorophenoxyacetic acid-containing medium decreased tryptamine levels and increased the bioconversion of secologanin to ajmalicine. Zeatin also enhan...

journal_title：Plant cell reports

authors： Decendit A,Petit G,Andreu F,Doireau P,Mérillon JM,Rideau M

更新日期：1993-10-01 00:00:00

abstract：:We have assessed the capacity of cultured protoplasts from two tissue sources of several commercially-grown broccoli cultivars to regenerate plants. A procedure that employs hypocotyl protoplasts and a culture medium with a high NAA:2,4-D auxin ratio was developed. The procedure permits highly efficient formation of c...

journal_title：Plant cell reports

authors： Kao HM,Keller WA,Gleddie S,Brown GG

更新日期：1990-10-01 00:00:00

abstract：KEY MESSAGE:Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Crosses between different genotypes and even between different species are commonly used in plant breeding...

journal_title：Plant cell reports

authors： Gimenez MD,Vazquez DV,Trepat F,Cambiaso V,Rodríguez GR

更新日期：2021-01-01 00:00:00

abstract：UNLABELLED:The quality and productivity of chrysanthemum are severely compromised by various abiotic stresses. Here, we describe the isolation of CdICE1 from Chrysanthemum dichrum using RACE PCR, which shared identical nucleotide of ICE1 ORF from Chrysanthemum grandiflorum variety 'Jinba'. CdICE1 contains a conserved b...

journal_title：Plant cell reports

authors： Chen L,Chen Y,Jiang J,Chen S,Chen F,Guan Z,Fang W

更新日期：2012-09-01 00:00:00

abstract：:The goal of the study was to characterize the optimal developmental stage of zygotic embryo expiants of the hardwood forest tree species yellow-poplar (Liriodendron tulipifera L.) for the initiation of embryogenic cultures, using morphological measurements and polypeptide profiles of the embryos. Developing zygotic em...

journal_title：Plant cell reports

authors： Sotak RJ,Sommer HE,Merkle SA

更新日期：1991-07-01 00:00:00

abstract：:Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles car...

journal_title：Plant cell reports

authors： Fitch MM,Manshardt RM,Gonsalves D,Slightom JL,Sanford JC

更新日期：1990-08-01 00:00:00

abstract：KEY MESSAGE:FLA, the homolog of ubiquitin-specific protease does not have deubiquitination activity, but it is essential for flower and chloroplast development in rice. Ubiquitin-specific proteases (UBPs) are widely distributed and highly conserved proteins and are also members of the most important family of deubiquit...

journal_title：Plant cell reports

authors： Ma X,Zhang J,Han B,Tang J,Cui D,Han L

更新日期：2019-03-01 00:00:00

abstract：:A protocol previously developed for B. napus microspore culture was modified to produce embryos from several lines of Brassica campestris. Bud size, genotype, media constituents, and incubation time and temperature were examined. Donor plants were grown in a growth cabinet at a day/night temperature of 10/5°C. Microsp...

journal_title：Plant cell reports

authors： Baillie AM,Epp DJ,Hutcheson D,Keller WA

更新日期：1992-06-01 00:00:00