Modified ELISA for the detection of neomycin phosphotransferase II in transformed plant species.

abstract：:Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos dev...

journal_title：Plant cell reports

authors： Xu N,Bewley JD

更新日期：1992-06-01 00:00:00

abstract：:A novel strategy for the production and maintenance of morphogenic callus for 1 year from mature leaf explants of apple has been developed using micropropagated primary leaves of cv. Golden Delicious. The technique required second generation adventitious buds produced from cultured primary leaves also produced from es...

journal_title：Plant cell reports

authors： Rugini E,Muganu M

更新日期：1998-04-01 00:00:00

abstract：:Hypocotyl explants of three leguminous forest tree species, Albizia amara, A. lucida and A. richardiana, have differentiated shoot buds on B5 basal medium. Maximum number of shoots per explant developed on basal medium augmented with 2,4-D (0.1 μM) in A. amara (2) and BA (10 μM) for both A. lucida (2) and A. richardia...

journal_title：Plant cell reports

authors： Tomar UK,Gupta SC

更新日期：1988-10-01 00:00:00

abstract：KEY MESSAGE:Overexpression of SoSnRK2.1 improved drought tolerance and growth of tobacco plants. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. In this research, SoSnRK2.1...

journal_title：Plant cell reports

authors： Phan TT,Sun B,Niu JQ,Tan QL,Li J,Yang LT,Li YR

更新日期：2016-09-01 00:00:00

abstract：KEY MESSAGE:Transgenic Arabidopsis and lettuce plants overexpressing AtHSP17.8 showed ABA-hypersensitive but abiotic stress-resistant phenotypes. ABA treatment caused a dramatic induction of early ABA-responsive genes in AtHSP17.8 -overexpressing transgenic lettuce. Plant small heat shock proteins function as chaperone...

journal_title：Plant cell reports

authors： Kim DH,Xu ZY,Hwang I

更新日期：2013-12-01 00:00:00

abstract：:The DNA-binding domain of the yeast transcriptional activator GAL4 was expressed in transgenic tobacco plants in order to attempt to obtain specific repression of reporter genes. Expression of the GUS and the NPTII gene was essentially the same regardless of the presence of a gene encoding a functional GAL4 DNA-bindin...

journal_title：Plant cell reports

authors： Reichel C,Feltkamp D,Walden R,Steinbiß HH,Schell J,Rosahl S

更新日期：1995-10-01 00:00:00

abstract：:Quantitative real-time polymerase chain reaction (PCR) assays were designed that enabled the zygosity of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) to be determined. The two zygosity assays, based on TaqMan technology that uses a fluorogenic probe which hybridizes to a PCR target...

journal_title：Plant cell reports

authors： Schmidt M,Parrott W

更新日期：2001-07-01 00:00:00

abstract：:Genetic transformation of arctic bramble (Rubus arcticus L.) was achieved utilizing a Ti-plasmid vector system of Agrobacterium tumefaciens. Internodal stem segments were inoculated with Agrobacterium strain EHA101 carrying a T-DNA with the CaMV 35 S promoter-gus-int marker gene from which β-glucuronidase (GUS) is exp...

journal_title：Plant cell reports

authors： Kokko HI,Kärenlampi SO

更新日期：1998-07-01 00:00:00

abstract：:High yields of viable protoplasts were obtained by enzymatic treatment from cotyledonary leaves of various greenhouse grown Phaseolus vulgaris L. cultivars. The protoplasts divided and formed cell clusters in a liquid medium. Early transfer before 10 days in the same medium was necessary for the development of cell co...

journal_title：Plant cell reports

authors： Crepy L,Barros LM,Valente VR

更新日期：1986-04-01 00:00:00

abstract：KEY MESSAGE:Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Crosses between different genotypes and even between different species are commonly used in plant breeding...

journal_title：Plant cell reports

authors： Gimenez MD,Vazquez DV,Trepat F,Cambiaso V,Rodríguez GR

更新日期：2021-01-01 00:00:00

abstract：:Shoot regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L.) was tested on MS basal medium supplemented with four different growth regulators. Regeneration frequencies for medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 60 μM 4amino-3, 5,6-picolinic acid (piclora...

journal_title：Plant cell reports

authors： Griffin JD,Dibble MS

更新日期：1995-08-01 00:00:00

abstract：:Emission and plant uptake of atmospheric nitrogen oxides (NO + NO(2)) significantly influence regional climate change by regulating the oxidative chemistry of the lower atmosphere, species composition and the recycling of carbon and nutrients, etc. Plant uptake of nitrogen dioxide (NO(2)) is concentration-dependent an...

journal_title：Plant cell reports

authors： Hu Y,Sun G

更新日期：2010-10-01 00:00:00

abstract：:From the in vitro hairy root cultures of a Datura candida hybrid, 19 tropane alkaloids have been identified using capillary gas-liquid chromatography and mass spectrometry. As in the parent plants, scopolamine is the major alkaloid. Two hitherto undescribed alkaloids have been detected and their structure tentatively ...

journal_title：Plant cell reports

authors： Christen P,Roberts MF,David Phillipson J,Evans WC

更新日期：1990-07-01 00:00:00

abstract：:Innovations in plant breeding like genome editing methods raised questions about the adequacy of established regulatory policies for plant breeding and biotechnology in view of these new breeding methods and the resulting products. Most countries follow the principle approach that only those plants will be regulated u...

journal_title：Plant cell reports

authors： Jorasch P

更新日期：2020-01-01 00:00:00

abstract：:Methods of shoot and plant development from cotyledon explants of the tomato cultivar 'Bonner Beste' and its mutant chloronerva are described. Cotyledon mesophyll protoplasts of the mutant chloronerva have been isolated and cultured, and induced to regenerate shoots and plants. By grafting regenerated shoots of the ch...

journal_title：Plant cell reports

authors： Koblitz H,Koblitz D

更新日期：1983-08-01 00:00:00

abstract：:Kidney vetch seedlings were induced to form hairy roots by inoculating their mesocotyls with the wild-type strain 15834 of Agrobacterium rhizogenes or with the A. tumefaciens strain C58C1 containing a binary vector system (the pRiA4b as a helper and the vector pCB1346 bearing a pTiC58-derived isopentenyl transferase g...

journal_title：Plant cell reports

authors： Stiller J,Sinec VN,Svoboda S,Němcová B,Macháčková I

更新日期：1992-07-01 00:00:00

abstract：KEY MESSAGE:MANNANASE7 gene in Brassica napus L. encodes a hemicellulose which located at cell wall or extracellular space and dehiscence-resistance can be manipulated by altering the expression of MANNANASE7. Silique dehiscence is an important physiological process in plant reproductive development, but causes heavy y...

journal_title：Plant cell reports

authors： Li YL,Yu YK,Zhu KM,Ding LN,Wang Z,Yang YH,Cao J,Xu LZ,Li YM,Tan XL

更新日期：2021-02-01 00:00:00

abstract：:Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...

journal_title：Plant cell reports

authors： Skirvin RM,Chu MC,Mann ML,Young H,Sullivan J,Fermanian T

更新日期：1986-08-01 00:00:00

abstract：KEY MESSAGE:Cadmium sensitivity in sultr1;1 - sultr1;2 double mutant with limiting sulfate supply is attributed to the decreased glutathione content that affected oxidative defense but not phytochelatins' synthesis. In plants, glutathione (GSH) homeostasis plays pivotal role in cadmium (Cd) detoxification. GSH is synth...

journal_title：Plant cell reports

authors： Liu X,Wu FH,Li JX,Chen J,Wang GH,Wang WH,Hu WJ,Gao LJ,Wang ZL,Chen JH,Simon M,Zheng HL

更新日期：2016-02-01 00:00:00

abstract：:A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...

journal_title：Plant cell reports

authors： Nussbaumer P,Kapétanidis I,Christen P

更新日期：1998-03-01 00:00:00

abstract：:Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...

journal_title：Plant cell reports

authors： Jorgensen JA,Weng J,Ho TH,Nguyen HT

更新日期：1992-10-01 00:00:00

abstract：:While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was...

journal_title：Plant cell reports

authors： Sorkina A,Bardosh G,Liu YZ,Fridman I,Schlizerman L,Zur N,Or E,Goldschmidt EE,Blumwald E,Sadka A

更新日期：2011-09-01 00:00:00

abstract：:We have assessed the capacity of cultured protoplasts from two tissue sources of several commercially-grown broccoli cultivars to regenerate plants. A procedure that employs hypocotyl protoplasts and a culture medium with a high NAA:2,4-D auxin ratio was developed. The procedure permits highly efficient formation of c...

journal_title：Plant cell reports

authors： Kao HM,Keller WA,Gleddie S,Brown GG

更新日期：1990-10-01 00:00:00

abstract：:Protoplasts isolated from cell suspensions of albinoMedicago borealis andM. sativa were fused chemically, using two methods, and electrically. Although a small scale method of chemical fusion gave the highest fusion frequency, electrofusion was the superior technique on the basis of throughput of green somatic hybrid ...

journal_title：Plant cell reports

authors： Gilmour DM,Davey MR,Cocking EC

更新日期：1989-05-01 00:00:00

abstract：:Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus inductio...

journal_title：Plant cell reports

authors： Utomo HS,Croughan SS,Croughan TP

更新日期：1995-01-01 00:00:00

abstract：:Somatic hybrids between Solanum tuberosum cv 'Brodick' and S. sanctae-rosae were selected for having elevated chromosome numbers, intermediate morphology and potato cyst nematode resistance (derived from S. sanctae-rosae). DNA was extracted from this material and subjected to inter-SSR PCR directed by oligonucleotide ...

journal_title：Plant cell reports

authors： Matthews D,McNicoll J,Harding K,Millam S

更新日期：1999-12-01 00:00:00

abstract：KEY MESSAGE:Drastic changes in soil water content can activate the short-term high expression of key enzyme-encoding genes involved in secondary metabolite synthesis thereby increasing the content of secondary metabolites. Bupleurum chinense DC. is a traditional medicinal herb that is famous for its abundant saikosapon...

journal_title：Plant cell reports

authors： Yang L,Zhao Y,Zhang Q,Cheng L,Han M,Ren Y,Yang L

更新日期：2019-09-01 00:00:00

abstract：:Reduced glutathione (GSH) was applied to prevent browning in shoot tip explants of apple (Malus pumila Mill.). Development was compared between shoot tips treated either by (a) dipping into 0.1 mM GSH solution prior to culture (dip treatment), (b) dipping into the GSH solution and transferring to a medium containing 0...

journal_title：Plant cell reports

authors： Nomura K,Matsumoto S,Masuda K,Inoue M

更新日期：1998-05-01 00:00:00

abstract：:Cell suspension cultures of chickpea (Cicer arietinum L.) were established from cultivars ILC 3279 and ILC 1929, resistant and susceptible towards the chickpea pathogenic fungus Ascochyta rabiei. The two cell culture lines possess identical growth properties and show high accumulation of the isoflavones biochanin A an...

journal_title：Plant cell reports

authors： Keßmann H,Barz W

更新日期：1987-02-01 00:00:00

abstract：:Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or ligh...

journal_title：Plant cell reports

authors： Mizukami H,Tomita K,Ohashi H

更新日期：1989-12-01 00:00:00