Efficient plant regeneration from hypocotyl protoplasts of broccoli (Brassica oleracea L. ssp. italica Plenck).

Abstract:

:We have assessed the capacity of cultured protoplasts from two tissue sources of several commercially-grown broccoli cultivars to regenerate plants. A procedure that employs hypocotyl protoplasts and a culture medium with a high NAA:2,4-D auxin ratio was developed. The procedure permits highly efficient formation of colonies that regenerate shoots at frequencies of 8-17% with two of the four cultivars tested. The time required for the development of plants from protoplasts was 8-11 weeks. No mtDNA rearrangements were observed among any of 17 analysed regenerants. Double-stranded RNAs were detected in mitochondrial DNA (mtDNA) preparations of some, but not all, regenerants of one of the cultivars.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Kao HM,Keller WA,Gleddie S,Brown GG

doi

10.1007/BF00232858

subject

Has Abstract

pub_date

1990-10-01 00:00:00

pages

311-5

issue

6

eissn

0721-7714

issn

1432-203X

journal_volume

9

pub_type

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