Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria x ananassa Duch.) using a range of explant types.

Abstract:

:The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf discs, petioles, roots and stipules. Regeneration from cv. Elsanta proved to be difficult from all explant material, although unpollinated ovaries proved to be a promising explant source, with 12% of the explants regenerating shoots. In cv. Eros, regeneration occurred only from root tissue. A comparison of the genetic background suggests that there is a strong genetic component amongst the different cultivars determining their regeneration capacity. The development of these regeneration systems provides a means to use almost the whole stock plant for the efficient genetic transformation of commercial strawberry varieties.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Passey AJ,Barrett KJ,James DJ

doi

10.1007/s00299-002-0530-4

keywords:

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

397-401

issue

5

eissn

0721-7714

issn

1432-203X

journal_volume

21

pub_type

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