Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria x ananassa Duch.) using a range of explant types.


:The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf discs, petioles, roots and stipules. Regeneration from cv. Elsanta proved to be difficult from all explant material, although unpollinated ovaries proved to be a promising explant source, with 12% of the explants regenerating shoots. In cv. Eros, regeneration occurred only from root tissue. A comparison of the genetic background suggests that there is a strong genetic component amongst the different cultivars determining their regeneration capacity. The development of these regeneration systems provides a means to use almost the whole stock plant for the efficient genetic transformation of commercial strawberry varieties.


Plant Cell Rep


Plant cell reports


Passey AJ,Barrett KJ,James DJ





Has Abstract


2003-01-01 00:00:00












  • Evolution of endogenous hormone concentration in embryogenic cultures of carrot during early expression of somatic embryogenesis.

    abstract::Embryogenic callus and suspension cultures of carrot (Daucus carota L., cv. Nantaise), growing on/in medium including 1 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), were transferred to medium with or without this plant growth regulator, to impair or induce, respectively, further development of somatic embryos. The en...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Jiménez VM,Guevara E,Herrera J,Bangerth F

    更新日期:2005-01-01 00:00:00

  • Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans.

    abstract:KEY MESSAGE:We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestans infection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different sig...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Fantino E,Segretin ME,Santin F,Mirkin FG,Ulloa RM

    更新日期:2017-07-01 00:00:00

  • The Arabidopsis CORI3 promoter contains two cis-acting regulatory regions required for transcriptional activity in companion cells.

    abstract::Companion cells are metabolically active and functionally specialized cells that behave as terminals for the transport of materials between phloem and the surrounding tissue. Although previous research has clarified the distinct function of companion cells, it is still largely unknown how plants establish and maintain...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Tsuwamoto R,Harada T

    更新日期:2011-09-01 00:00:00

  • Production of kanamycin resistant rice tissues following DNA uptake into protoplasts.

    abstract::Rice protoplasts (Oryza sativa L. v Taipei 309) have been transformed to kanamycin resistance following uptake of pCaMVNEO induced by electroporation, PEG and PEG combined with electroporation. Protoplast-derived colonies selected on medium containing 100 μg/ml of kanamycin expressed NPTII activity, and contained DNA ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Yang H,Zhang HM,Davey MR,Mulligan BJ,Cocking EC

    更新日期:1988-10-01 00:00:00

  • Transformation of floral organs with GFP in Medicago truncatula.

    abstract:: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this met...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Kamaté K,Rodriguez-Llorente ID,Scholte M,Durand P,Ratet P,Kondorosi E,Kondorosi A,Trinh TH

    更新日期:2000-06-01 00:00:00

  • Vegetative propagation of Quercus suber L. by somatic embryogenesis. II. Plant regeneration from selected cork oak trees.

    abstract::The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segmen...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hernández I,Celestino C,Alegre J,Toribio M

    更新日期:2003-04-01 00:00:00

  • Phytochelatin accumulation and stress tolerance in tomato cells exposed to cadmium.

    abstract::Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gupta SC,Goldsbrough PB

    更新日期:1990-12-01 00:00:00

  • Isolation of protoplasts and regeneration of callus from suspension cultures of cultivated beets.

    abstract::Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were fou...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Bhat SR,Ford-Lloyd BV,Callow JA

    更新日期:1985-12-01 00:00:00

  • Modified ELISA for the detection of neomycin phosphotransferase II in transformed plant species.

    abstract:: Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the us...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: McKenzie MJ,Mett V,Jameson PE

    更新日期:2000-01-01 00:00:00

  • Growth and rosmarinic acid production in cell suspension cultures of Salvia officinalis L.

    abstract::Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hippolyte I,Marin B,Baccou JC,Jonard R

    更新日期:1992-04-01 00:00:00

  • Separation of root nodule cells and identification of tissue-specific genes.

    abstract::The interior tissues of the alfalfa (Medicago sativa) root nodule differ in form and function from the peripheral layers. The interior tissues are specialized for the fixation of nitrogen in cells infected by rhizobia. In contrast, the peripheral nodule tissues perform roles that assist the interior tissues: they prov...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ganter G,Duquette M,Dunn K

    更新日期:2000-09-01 00:00:00

  • Desiccation of microspore derived embryos of oilseed rape (Brassica napus L.).

    abstract::Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or liquid medium gave s...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Senaratna T,Kott L,Beversdorf WD,McKersie BD

    更新日期:1991-09-01 00:00:00

  • Thermoinduction of genes encoding the enzymes of gibberellin biosynthesis and a putative negative regulator of gibberellin signal transduction in Eustoma grandiflorum.

    abstract::Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Mino M,Oka M,Tasaka Y,Iwabuchi M

    更新日期:2003-09-01 00:00:00

  • In vitro storage of cedar shoot cultures under minimal growth conditions.

    abstract::We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Renau-Morata B,Arrillaga I,Segura J

    更新日期:2006-07-01 00:00:00

  • Increased formation of cinnamoyl putrescines by fedbatch fermentation of cell suspension cultures of Nicotiana tabacum.

    abstract::The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Schiel O,Jarchow-Redecker K,Piehl GW,Lehmann J,Berlin J

    更新日期:1984-02-01 00:00:00

  • DNA fingerprinting of red clover (Trifolium pratense L.) with Jeffrey's probes: detection of somaclonal variation and other applications.

    abstract::DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Nelke M,Nowak J,Wright JM,McLean NL

    更新日期:1993-12-01 00:00:00

  • Micropropagation and field performance of Yucca valida.

    abstract::Yucca valida is an important potential source of steroidal saponins closely related to Yucca schidigera, the species that is commercially exploited from the wild as a source of steroidal extracts. Neither of the species has been domesticated mainly because of their slow growth and long life span before harvesting. Her...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Arce-Montoya M,Rodríguez-Alvarez M,Hernández-González JA,Robert ML

    更新日期:2006-08-01 00:00:00

  • Gynogenic plant regeneration from unpollinated flower explants of Eragrostis tef (Zuccagni) Trotter.

    abstract::Tef [Eragrostis tef (Zucc.) Trotter] is the most important cereal in Ethiopia. In its wild relative E. mexicana, regeneration of six green plants resulted from culture of 121 non-pollinated immature pistils. In the allotetraploid crop species tef, however, only callus and root formation was obtained by this method. By...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gugsa L,Sarial AK,Lörz H,Kumlehn J

    更新日期:2006-12-01 00:00:00

  • Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture.

    abstract::The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in th...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Soriano M,Cistué L,Castillo AM

    更新日期:2008-05-01 00:00:00

  • Contrasting pattern of somatic and zygotic embryo development in alfalfa (Medicago sativa L.) as revealed by scanning electron microscopy.

    abstract::Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos dev...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Xu N,Bewley JD

    更新日期:1992-06-01 00:00:00

  • Plant lipid environment and membrane enzymes: the case of the plasma membrane H+-ATPase.

    abstract::Several lipid classes constitute the universal matrix of the biological membranes. With their amphipathic nature, lipids not only build the continuous barrier that confers identity to every cell and organelle, but they are also active actors that modulate the activity of the proteins immersed in the lipid bilayer. The...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审


    authors: Morales-Cedillo F,González-Solís A,Gutiérrez-Angoa L,Cano-Ramírez DL,Gavilanes-Ruiz M

    更新日期:2015-04-01 00:00:00

  • Development of a CAPS marker system for genotyping European pear cultivars harboring 17 S alleles.

    abstract::The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Moriya Y,Yamamoto K,Okada K,Iwanami H,Bessho H,Nakanishi T,Takasaki T

    更新日期:2007-03-01 00:00:00

  • Auxin-polyamine interaction with the calcium-controlled secretion of peroxidase by sugarbeet cells.

    abstract::Spermidine and ornithine given to normal auxin-requiring cell suspensions of sugarbeet inhibited peroxidase secretion in the absence of Ca(2+). Habituated (organogenic or not) cells did not respond. Both compounds counteracted the Ca(2+) - promoted enzyme secretion by three cell lines. Auxins (2,4-D and BSAA) did not ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Kevers C,Gaspar T,Penel C,Greppin H

    更新日期:1985-05-01 00:00:00

  • Expression of a salt-induced protein (SALT) in suspension-cultured cells and leaves of rice following exposure to fungal elicitor and phytohormones.

    abstract::Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Kim ST,Kim SG,Hwang DH,Kang SY,Koo SC,Cho MJ,Kang KY

    更新日期:2004-10-01 00:00:00

  • Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum Mill.

    abstract::In long-term callus cultures of Cyclamen persicum Mill. two types of tissue could be distinguished. One type featured a brown suberised outer layer and was poorly organogenic. The other type was yellowish in appearance and gave rise to many shoot buds. Both types co-existed on the same callus, the former prevailing. S...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Dillen W,Dijkstra I,Oud J

    更新日期:1996-03-01 00:00:00

  • Improved nitrogen use efficiency in transgenic sugarcane: phenotypic assessment in a pot trial under low nitrogen conditions.

    abstract:KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Snyman SJ,Hajari E,Watt MP,Lu Y,Kridl JC

    更新日期:2015-05-01 00:00:00

  • Control of foreign polypeptide localization in specific layers of protein body type I in rice seed.

    abstract:KEY MESSAGE:Prolamin-GFP fusion proteins, expressed under the control of native prolamin promoters, were localized in specific layers of PB-Is. Prolamin-GFP fusion proteins were gradually digested from outside by pepsin digestion. In rice seed endosperm, protein body type I (PB-I) has a layered structure consisting of ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Sasou A,Shigemitsu T,Saito Y,Tanaka M,Morita S,Masumura T

    更新日期:2016-06-01 00:00:00

  • Non-antibiotic, efficient selection for alfalfa genetic engineering.

    abstract::A selectable marker gene (SMG), usually conferring resistance to an antibiotic or herbicide, is generally introduced into the plant cells with the gene(s) for the trait of interest to allow only the cells that have integrated and express the foreign sequences to regenerate into a plant. The availability of several SMG...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Rosellini D,Capomaccio S,Ferradini N,Savo Sardaro ML,Nicolia A,Veronesi F

    更新日期:2007-07-01 00:00:00

  • Plant regeneration fromArabidopsis thaliana protoplasts.

    abstract::Arabidopsis thaliana protoplasts, isolated from a cell suspension, regenerated cell walls and then exhibited sustained cell divisions. Within 10 days after isolation as many as 40% of the protoplast-derived cells divided. Protoplast-derived calli exhibited a wide range of developmental responses, including apparent em...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ford KG

    更新日期:1990-01-01 00:00:00

  • Production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker.

    abstract::The use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. MAT (multi-auto-transformation) vector system has been one of the different st...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Khan RS,Ntui VO,Chin DP,Nakamura I,Mii M

    更新日期:2011-04-01 00:00:00