Abstract：
:The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf discs, petioles, roots and stipules. Regeneration from cv. Elsanta proved to be difficult from all explant material, although unpollinated ovaries proved to be a promising explant source, with 12% of the explants regenerating shoots. In cv. Eros, regeneration occurred only from root tissue. A comparison of the genetic background suggests that there is a strong genetic component amongst the different cultivars determining their regeneration capacity. The development of these regeneration systems provides a means to use almost the whole stock plant for the efficient genetic transformation of commercial strawberry varieties.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Passey AJ,Barrett KJ,James DJdoi
10.1007/s00299-002-0530-4keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
397-401issue
5eissn
0721-7714issn
1432-203Xjournal_volume
21pub_type
杂志文章abstract：:In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050459
更新日期：1998-05-01 00:00:00
abstract：:A reliable protocol for the transformation of cultivated sunflower (Helianthus annuus L.) has been established, based on microprojectile bombardment of half shoot apices in combination with Agrobacterium tumefaciens coculture. Transgenic shoots have been obtained from 5 inbred lines, although transformation efficienci...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233766
更新日期：1994-12-01 00:00:00
abstract：KEY MESSAGE:The radish WRKY gene family was genome-widely identified and played critical roles in response to multiple abiotic stresses. The WRKY is among the largest transcription factors (TFs) associated with multiple biological activities for plant survival, including control response mechanisms against abiotic stre...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2190-4
更新日期：2017-11-01 00:00:00
abstract：:A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1230-3
更新日期：2012-06-01 00:00:00
abstract：:We previously reported that Arabidopsis indole-3-acetic acid (IAA)-methyltransferase-1 (IAMT1) catalyzes the conversion of IAA, an essential phytohormone, to methyl-IAA (MeIAA) and that IAMT1 plays an important role in leaf development. Here, we present the possible mechanisms of action of MeIAA in Arabidopsis. We sho...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0458-9
更新日期：2008-03-01 00:00:00
abstract：:A double-gene construct with one chitinase and one beta-1,3-glucanase gene from barley, both driven by enhanced 35S promoters, was transformed into oilseed rape. From six primary transformants expressing both transgenes 10 doubled haploid lines were produced and studied for five generations. The number of inserted cop...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0153-2
更新日期：2006-09-01 00:00:00
abstract：:Shoot and leaf segments of a non-regenerable Medicago sativa L. genotype were cocultivated with the "shooty" mutant of Agrobacterium tumefaciens carrying the pGV 2206 plasmid. Transformed callus lines were selected and regenerated on the hormone free B5 medium. Southern blot analysis demonstrated integration of T-DNA ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269987
更新日期：1990-04-01 00:00:00
abstract：:A method for regenerating pigeonpea [Cajanus cajan (L.) Millsp.] plants has been developed using distal cotyledonary segments of mature seeds as explants. A large number of shoot buds were induced directly from explants of genotypes T-15-15 and GAUT-82-90 when cultured on six different basal media fortified with 22.2 ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050469
更新日期：1998-06-01 00:00:00
abstract：:Isolated ovules from the maize homozygous recessive brown midrib (bm3) were in vitro pollinated by pollen carrying the dominant allele - and the purple embryo marker (PEM). The colour characters of the embryos and kernels corresponded to the results of control pollination and confirmed the process of double fertilizat...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00267652
更新日期：1981-08-01 00:00:00
abstract：:A T-DNA insertional mutant OsPE of rice gives twin and triplet seedlings in up to 20 % of the seeds. Detailed cytological and histological analysis of OsPE indicated normal male and female gametogenesis in the OsPE mutant. Confocal laser scanning microscopic (CLSM) analysis of the developing seeds of OsPE showed multi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1291-3
更新日期：2012-10-01 00:00:00
abstract：:The activity and activation potential of polyphenoloxidase (PPO, E.C. 1.10.3.1.) of tissue from shoot tips, adult leaves and embryogenic and non-embryogenic cell suspension cultures of Euphorbia pulcherrima was investigated using an oxygen probe technique. PPO derived from differentiated in vivo plant tissue (shoot ti...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234048
更新日期：1995-04-01 00:00:00
abstract：:DNA transfer into zygotic embryos of Eucalyptus globulus by microprojectile bombardment was studied with two devices: a gunpowder apparatus and a compressed-helium system. Using, as a test, the transient expression of a reporter gene, we optimized the physical and biological conditions of bombardment. Six-day-old cult...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232737
更新日期：1995-07-01 00:00:00
abstract：KEY MESSAGE:Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Crosses between different genotypes and even between different species are commonly used in plant breeding...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02624-x
更新日期：2021-01-01 00:00:00
abstract：:A reliable and efficient system for transformation and regeneration of 'Chardonnay' (Vitis vinifera L.) plants via microprojectile bombardment was developed. Improvements over the previous biolistic transformation system included: (1) the use of gold particles for bombardment; (2) step-wise selection at 10 then 15 mg/...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0682-x
更新日期：2003-11-01 00:00:00
abstract：:Transgenic tobacco plants expressing an inhibitor of ubiquitin-dependent protein degradation, ubiquitin variant ubR48, spontaneously formed necrotic lesions and displayed altered responsiveness to tobacco mosaic virus attack. These plants were analyzed for the accumulation of defense-related compounds and the expressi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050501
更新日期：1998-08-01 00:00:00
abstract：:The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1183-y
更新日期：2012-04-01 00:00:00
abstract：KEY MESSAGE:The dynamic alterations of the physiological and molecular processes in reproductive stage soybean indicated the dramatic impact caused by drought. Drought is a major abiotic stress that limits soybean (Glycine max) production. Most prior studies were focused on either model species or crops that are at the...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-018-2332-3
更新日期：2018-12-01 00:00:00
abstract：:Modern powerful techniques in plant biotechnology have been developed in lilies (Lilium spp., Liliaceae) to propagate, improve and make new phenotypes. Reliable in vitro culture methods are available to multiply lilies rapidly and shorten breeding programs. Lilium is also an ideal model plant to study in vitro pollina...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-016-2017-8
更新日期：2016-09-01 00:00:00
abstract：:Callus cultures from cotyledon explants were established and maintained in culture for more than two years. After several months callus cultures were transferred into liquid medium and cultured as cell suspensions. Protoplasts were isolated from these cell suspension cultures and cultured in a liquid medium. After for...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269184
更新日期：1982-06-01 00:00:00
abstract：:Suspension cultured cells of the moss, Barbula unguiculata, grow actively in both light and dark culture. Light-grown cells contain chlorophyll and exhibit an undifferentiated callus form. When cells are transferred to a dark condition, they develop into protonemata. Protonemata formation in the dark can be inhibited ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272039
更新日期：1989-03-01 00:00:00
abstract：:High yields of protoplasts were obtained from leaves of aseptically grown plants and calli originated from different explants, in several cultivars of Cajanus cajan L. The protoplasts divided to form cell clusters in modified KM 8p medium and developed to protocolonies after dilution with liquid Caboche's medium withi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232691
更新日期：1992-08-01 00:00:00
abstract：:Treatment of Allium cepa L. cellsuspension cultures with a biotic elicitor derived from the fungus Botrytis cinerea, resulted in phytoalexin synthesis. Two phytoalexins, 5-octylcyclopenta-1,3-dione and 5-hexyl-cyclopenta-1,3-dione, were accumulated in cultured onion cells. Removal of extracellular Ca(2+) by the calciu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231593
更新日期：1996-09-01 00:00:00
abstract：:Protoplasts isolated enzymatically from seedling roots, hypocotyls and cotyledons of Lotus corniculatus L. produced callus which underwent prolific shoot regeneration. The rapidity and ease of recovering plants from protoplast-derived tissues makes this forage legume an attractive experimental system for genetic manip...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270177
更新日期：1983-04-01 00:00:00
abstract：:The interior tissues of the alfalfa (Medicago sativa) root nodule differ in form and function from the peripheral layers. The interior tissues are specialized for the fixation of nitrogen in cells infected by rhizobia. In contrast, the peripheral nodule tissues perform roles that assist the interior tissues: they prov...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000216
更新日期：2000-09-01 00:00:00
abstract：:Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a particularly high abundance of fl...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0255-x
更新日期：2007-04-01 00:00:00
abstract：KEY MESSAGE:Tetraploid clementine displays mainly tetrasomic inheritance. Genetic structures of 2n SDR and 2 × gametes from DD clementine are complementary and will guides triploids citrus breeding strategies. Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be reco...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1972-4
更新日期：2016-08-01 00:00:00
abstract：:Cotyledon explants from zygotic embryos of Panax ginseng produced somatic embryos on Murashige and Skoog basal medium without growth regulators. Somatic embryos developed directly from epidermal cells at the cotyledon base. Somatic embryos were always formed from the side of the cotyledon opposite to the one attached ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050331
更新日期：1997-10-01 00:00:00
abstract：:A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232265
更新日期：1990-12-01 00:00:00
abstract：:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000198
更新日期：2000-09-01 00:00:00
abstract：:Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been as extensively studied. We examined the effect of brassinolide on the kinetics and final division frequencies of regenerating leaf mesophyll protoplasts of Petunia hybrida Vilm v. Coman...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050510
更新日期：1998-09-01 00:00:00
