The promoter of the Arabidopsis thaliana BAN gene is active in proanthocyanidin-accumulating cells of the Brassica napus seed coat.


:As part of an ongoing research program dedicated to the understanding of proanthocyanidin (PA) accumulation in Brassica napus seed coat, transgenic rapeseed plants carrying a 2.3-kb fragment of the Arabidopsis thaliana BAN promoter (ProAtBAN) fused to the uidA reporter gene (GUS) were generated. Analysis of these plants revealed that ProAtBAN was activated in B. napus seed coat, following a spatio-temporal pattern that was very similar to the PA deposition profile in rapeseed and also to the one previously described in Arabidopsis. ProAtBAN activity occurred as soon as the early stages of embryogenesis and was restricted to the cells where PAs were shown to accumulate. Therefore, the Arabidopsis BAN promoter can be used to trigger gene expression in B. napus seed coat for both genetic engineering and functional validation of candidate genes. In addition, these data strongly suggest that the transcriptional regulatory network of the BAN gene is conserved between Arabidopsis and rapeseed. This is consistent with the fact that similarity searches of the public rapeseed sequence databases allowed recovering the rapeseed homologs for several BAN regulators, namely TT1, TT2, TT8, TT16 and TTG1, which have been previously described in Arabidopsis.


Plant Cell Rep


Plant cell reports


Nesi N,Lucas MO,Auger B,Baron C,Lécureuil A,Guerche P,Kronenberger J,Lepiniec L,Debeaujon I,Renard M




Has Abstract


2009-04-01 00:00:00












  • Self-compatibility in 'Cristobalina' sweet cherry is not associated with duplications or modified transcription levels of S-locus genes.

    abstract::Sweet cherry shows S-RNase-based gametophytic self-incompatibility, which prevents self- and cross-fertilization between genetically related individuals. The specificity of the self-incompatible reaction is determined by two genes located in the S-locus. These encode a pistil-expressed ribonuclease (S-RNase) that inhi...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Wünsch A,Tao R,Hormaza JI

    更新日期:2010-07-01 00:00:00

  • Improvement of protoplast culture protocols for Beta vulgaris L. (sugar beet).

    abstract::The effects of NaCl, feeder cells and the embedding of protoplasts in calcium alginate have been investigated in an attempt to improve culture conditions of recalcitrant sugar beet (Beta vulgaris L.) mesophyll protoplasts. While the use of NaCl in all instances proved detrimental to protoplast development, the other t...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hall RD,Pedersen C,Krens FA

    更新日期:1993-04-01 00:00:00

  • In vitro propagation through axillary bud multiplication in different mulberry genotypes.

    abstract::Axillary buds from 5 genotypes of mulberry belonging to 4 species were cultured on modified MS basal medium. A total of 30 media combinations were tried for all the genotypes. The response of axillary buds and the requirement for growth regulators varied with genotype. In Morus indica BAP (0.25-0.5 mg/l), and in M. al...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Jain AK,Dandin SB,Sengupta K

    更新日期:1990-05-01 00:00:00

  • In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir.

    abstract::An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Guo B,Gao M,Liu CZ

    更新日期:2007-03-01 00:00:00

  • The shikonin derivatives and pyrrolizidine alkaloids in hairy root cultures of Lithospermum canescens (Michx.) Lehm.

    abstract::Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of a...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Pietrosiuk A,Sykłowska-Baranek K,Wiedenfeld H,Wolinowska R,Furmanowa M,Jaroszyk E

    更新日期:2006-10-01 00:00:00

  • Stability of tissue culture medium pH as a function of autoclaving, time, and cultured plant material.

    abstract::Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Skirvin RM,Chu MC,Mann ML,Young H,Sullivan J,Fermanian T

    更新日期:1986-08-01 00:00:00

  • Development of somatic hybrids Solanum × michoacanum Bitter. (Rydb.) (+) S. tuberosum L. and autofused 4x S. × michoacanum plants as potential sources of late blight resistance for potato breeding.

    abstract:KEY MESSAGE:Phytophthora infestans resistant somatic hybrids of S. × michoacanum (+) S. tuberosum and autofused 4 x S. × michoacanum were obtained. Our material is promising to introgress resistance from S. × michoacanum into cultivated potato background. Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Smyda P,Jakuczun H,Dębski K,Sliwka J,Thieme R,Nachtigall M,Wasilewicz-Flis I,Zimnoch-Guzowska E

    更新日期:2013-08-01 00:00:00

  • Genetic transformation of Populus nigra by Agrobacterium tumefaciens.

    abstract::Two clones of Populus nigra L. were tested in vivo and in vitro for their susceptibility to three different Agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. Strain C58 proved to be the most virulent.Various parameters affecting Agrobacterium-mediated transformation of P. ni...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Confalonieri M,Balestrazzi A,Bisoffi S

    更新日期:1994-02-01 00:00:00

  • Production of phosphinothricin-tolerant rice (Oryza sativa L.) through the application of phosphinothricin as growth regulator.

    abstract:: A novel procedure has been developed to produce rice (Oryza sativa L.) tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. First, sublethal and lethal concentrations of PPT on 7-day-old seedlings were determined and morphogenetic events in response to the PPT treatment evaluated. Differen...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Toldi O,Tóth S,Oreifig AS,Kiss E,Jenes B

    更新日期:2000-12-01 00:00:00

  • Plant regeneration from callus and protoplasts of Brassica nigra (IC 257) through somatic embryogenesis.

    abstract::A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gupta V,Agnihotri A,Jagannathan V

    更新日期:1990-12-01 00:00:00

  • Development of multiple embryos in polyembryonic insertional mutant OsPE of rice.

    abstract::A T-DNA insertional mutant OsPE of rice gives twin and triplet seedlings in up to 20 % of the seeds. Detailed cytological and histological analysis of OsPE indicated normal male and female gametogenesis in the OsPE mutant. Confocal laser scanning microscopic (CLSM) analysis of the developing seeds of OsPE showed multi...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Paul P,Awasthi A,Kumar S,Verma SK,Prasad R,Dhaliwal HS

    更新日期:2012-10-01 00:00:00

  • Successful recovery of transgenic cowpea (Vigna unguiculata) using the 6-phosphomannose isomerase gene as the selectable marker.

    abstract::A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-d...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Bakshi S,Saha B,Roy NK,Mishra S,Panda SK,Sahoo L

    更新日期:2012-06-01 00:00:00

  • Physiological and transcriptomic responses of reproductive stage soybean to drought stress.

    abstract:KEY MESSAGE:The dynamic alterations of the physiological and molecular processes in reproductive stage soybean indicated the dramatic impact caused by drought. Drought is a major abiotic stress that limits soybean (Glycine max) production. Most prior studies were focused on either model species or crops that are at the...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Xu C,Xia C,Xia Z,Zhou X,Huang J,Huang Z,Liu Y,Jiang Y,Casteel S,Zhang C

    更新日期:2018-12-01 00:00:00

  • Quantitative trait loci from identification to exploitation for crop improvement.

    abstract::Advancement in the field of genetics and genomics after the discovery of Mendel's laws of inheritance has led to map the genes controlling qualitative and quantitative traits in crop plant species. Mapping of genomic regions controlling the variation of quantitatively inherited traits has become routine after the adve...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审


    authors: Kumar J,Gupta DS,Gupta S,Dubey S,Gupta P,Kumar S

    更新日期:2017-08-01 00:00:00

  • Level of tissue differentiation influences the activation of a heat-inducible flower-specific system for genetic containment in poplar (Populus tremula L.).

    abstract:KEY MESSAGE:Differentiation level but not transgene copy number influenced activation of a gene containment system in poplar. Heat treatments promoted CRE gene body methylation. The flower-specific transgene deletion was confirmed. Gene flow between genetic modified trees and their wild relatives is still motive of con...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hoenicka H,Lehnhardt D,Nunna S,Reinhardt R,Jeltsch A,Briones V,Fladung M

    更新日期:2016-02-01 00:00:00

  • Elicitation of benzophenanthridine alkaloid synthesis in Eschscholtzia cell cultures.

    abstract::Quaternary benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelirubine, chelilutine and macarpine) are specifically induced by cell wall components of Penicillium and Saccharomyces in a colorless strain of Eschscholtzia californica cell suspension cultures. Classical elicitors such as the Phytophthora mega...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Schumacher HM,Gundlach H,Fiedler F,Zenk MH

    更新日期:1987-12-01 00:00:00

  • Characterization of salt tolerant alfalfa (Medicago sativa L.) plants regenerated from salt tolerant cell lines.

    abstract::Salt tolerant cell lines have been selected from Medicago sativa, by a single step selection process on tissue culture medium containing 1% NaCl. Plants regenerated from these lines show improved salt tolerance compared to parent plants. The regenerated plants are vigorous, have flowered and are self fertile. The cell...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Winicov I

    更新日期:1991-12-01 00:00:00

  • Callus formation from protoplasts of a sugarbeet cell suspension culture.

    abstract::Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protopla...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Szabados L,Gaggero C

    更新日期:1985-08-01 00:00:00

  • β-glucuronidase gene expression and mRNA stability in oat protoplasts.

    abstract::Protoplasts derived from oat (Avena sativa L.) suspension culture cells (7 days after subculturing) were electroporated with plasmid DNA containing the Escherichia coli uidA gene encoding the ß-glucuronidase reporter enzyme. Consistently high enzyme activity was observed with electroporation conditions of 500 μF and 1...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Higgs DC,Colbert JT

    更新日期:1993-05-01 00:00:00

  • Efficient callus formation and plant regeneration from leaves of oats (Avena sativa L.).

    abstract::An efficient plant regeneration system from leaf-derived callus in 6 genotypes of Avena sativa L. has been established. Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. Plants were regenerated from the regenerable callus and grown to maturity. The frequency of regenerable callus f...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Chen H,Xu G,Loschke DC,Tomaska L,Rolfe BG

    更新日期:1995-03-01 00:00:00

  • Use of an ultrasound cell retention system for the size fractionation of somatic embryos of woody species.

    abstract:: The potential of ultrasonic standing waves for trapping suspended particles was utilized successfully for differential size fractionation of plant somatic embryos. In a flow-through resonator equipped with a 200-kHz piezoceramic transducer, embryos of different sizes corresponding to different developmental stages co...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Maitz M,Trampler F,Gröschl M,da Câmara Machado A,Laimer da Câmara Machado M

    更新日期:2000-11-01 00:00:00

  • Expression of an immunogenic LTB-based chimeric protein targeting Zaire ebolavirus epitopes from GP1 in plant cells.

    abstract:KEY MESSAGE:An antigenic protein targeting two epitopes from the Zaire ebolavirus GP1 protein was expressed in plant cells rendering an antigen capable of inducing humoral responses in mouse when administered subcutaneously or orally. The 2014 Ebola outbreak made clear that new treatments and prophylactic strategies to...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ríos-Huerta R,Monreal-Escalante E,Govea-Alonso DO,Angulo C,Rosales-Mendoza S

    更新日期:2017-02-01 00:00:00

  • Shoot apex explants for induction of somatic embryogenesis in mature Quercus robur L. trees.

    abstract::A procedure for inducing somatic embryos in shoot apex explants (2 mm) excised from shoot proliferation cultures established from adult oak trees (Quercus robur) was investigated. Embryogenesis was induced in shoot tip as well as leaf explants in three out of the five genotypes evaluated. Somatic embryos were formed b...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: San-José MC,Corredoira E,Martínez MT,Vidal N,Valladares S,Mallón R,Vieitez AM

    更新日期:2010-06-01 00:00:00

  • Overexpression of wheat dehydrin DHN-5 enhances tolerance to salt and osmotic stress in Arabidopsis thaliana.

    abstract::Late Embryogenesis Abundant (LEA) proteins are associated with tolerance to water-related stress. A wheat (Triticum durum) group 2 LEA proteins, known also as dehydrin (DHN-5), has been previously shown to be induced by salt and abscisic acid (ABA). In this report, we analyze the effect of ectopic expression of Dhn-5 ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Brini F,Hanin M,Lumbreras V,Amara I,Khoudi H,Hassairi A,Pagès M,Masmoudi K

    更新日期:2007-11-01 00:00:00

  • Systems biology of seeds: deciphering the molecular mechanisms of seed storage, dormancy and onset of germination.

    abstract::Seeds are heterogeneous storage reserves with wide array of storage compounds that include various soluble carbohydrates, starch polymer, storage proteins and lipids. These stored reserves comprise 70% of the world's caloric intake in the form of food and animal feed produced through sustainable agriculture, which con...

    journal_title:Plant cell reports

    pub_type: 社论


    authors: Sreenivasulu N

    更新日期:2017-05-01 00:00:00

  • Polyamine biosynthesis during somatic embryogenesis in interior spruce (Picea glauca x Picea engelmannii complex).

    abstract::Putrescine, spermidine, and spermine levels during somatic embryogenesis of interior spruce (Picea glauca x Picea engelmannii complex) were quantified On abscisic acid supplemented growth medium putrescine and spermidine levels increased two-fold coinciding with maturation of the early somatic embryos to globular embr...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Amarasinghe V,Dhami R,Carlson JE

    更新日期:1996-03-01 00:00:00

  • Changes in DNA composition during cell differentiation in a green alga.

    abstract::In the coenobia-forming green alga, Hydrodictyon reticulatum, changes in DNA composition during growth and differentiation of reproductive cells were followed. A "loss" of DNA per nucleus could be attributed to underreplication of an amplified DNA fraction being part of a (GC)-rich satellite band in CsCl gradients of ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ssymank V

    更新日期:1983-10-01 00:00:00

  • Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens.

    abstract::We report here an efficient Arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. Subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces see...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: van der Graaff E,Hooykaas PJ

    更新日期:1996-04-01 00:00:00

  • Cryopreservation of in vitro-grown axillary shoot-tip meristems of mint (Mentha spicata L.) by encapsulation vitrification.

    abstract:: Alginate-coated meristems from in vitro-grown axillary buds of mint (Mentha spicata L.) were successfully cryopreserved by vitrification. Excised meristems from nodal segments cold hardened at 4  °C for 3 weeks were encapsulated and osmoprotected by a mixture of 2 M glycerol plus 0.4 M sucrose. These meristems were d...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hirai D,Sakai A

    更新日期:1999-12-01 00:00:00

  • Seed development of Jatropha curcas L. (Euphorbiaceae): integrating anatomical, ultrastructural and molecular studies.

    abstract:KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Soares EL,Lima MLB,Nascimento JRS,Soares AA,Coutinho ÍAC,Campos FAP

    更新日期:2017-11-01 00:00:00