Abstract:
:As part of an ongoing research program dedicated to the understanding of proanthocyanidin (PA) accumulation in Brassica napus seed coat, transgenic rapeseed plants carrying a 2.3-kb fragment of the Arabidopsis thaliana BAN promoter (ProAtBAN) fused to the uidA reporter gene (GUS) were generated. Analysis of these plants revealed that ProAtBAN was activated in B. napus seed coat, following a spatio-temporal pattern that was very similar to the PA deposition profile in rapeseed and also to the one previously described in Arabidopsis. ProAtBAN activity occurred as soon as the early stages of embryogenesis and was restricted to the cells where PAs were shown to accumulate. Therefore, the Arabidopsis BAN promoter can be used to trigger gene expression in B. napus seed coat for both genetic engineering and functional validation of candidate genes. In addition, these data strongly suggest that the transcriptional regulatory network of the BAN gene is conserved between Arabidopsis and rapeseed. This is consistent with the fact that similarity searches of the public rapeseed sequence databases allowed recovering the rapeseed homologs for several BAN regulators, namely TT1, TT2, TT8, TT16 and TTG1, which have been previously described in Arabidopsis.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Nesi N,Lucas MO,Auger B,Baron C,Lécureuil A,Guerche P,Kronenberger J,Lepiniec L,Debeaujon I,Renard Mdoi
10.1007/s00299-008-0667-xsubject
Has Abstractpub_date
2009-04-01 00:00:00pages
601-17issue
4eissn
0721-7714issn
1432-203Xjournal_volume
28pub_type
杂志文章abstract::Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231624
更新日期:1994-09-01 00:00:00
abstract::The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1183-y
更新日期:2012-04-01 00:00:00
abstract::In leek (Allium ampeloprasum L.) a cyclic system of somatic embryogenesis was developed. Somatic embryos used for cyclic embryogenesis were able to develop the same type of embryogenic callus as zygotic embryos in the primary cycle. For the first time a comparison of the efficiencies of both expiants was made. Ten fam...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233638
更新日期:1995-01-01 00:00:00
abstract:KEY MESSAGE:MsCBL4 expression in tobacco enhanced its salt and saline-alkali stress tolerance by regulating calcium accumulation in roots, indicating the important role of calcium metabolism in plant saline-alkali stress tolerance The calcineurin B-like (CBL) family of proteins play important roles in plant abiotic str...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02543-x
更新日期:2020-08-01 00:00:00
abstract::Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). How...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269060
更新日期:1989-12-01 00:00:00
abstract:KEY MESSAGE:The recovery of non-functional-enhanced green fluorescence protein can be used as indicator to facilitate the identification of mutants generated by CRISPR/Cas9. The CRISPR/Cas9 system is a powerful tool for genome editing and it has been employed to knock out genes of interest in multiple plant species. Id...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02465-3
更新日期:2019-12-01 00:00:00
abstract::Approximately 10(6) protoplast-derived cell colonies of sainfoin were stressed with streptomycin and two resistant colonies were recovered. Plants regenerated from these colonies could be recallused on streptomycin-containing medium three years after growth in the absence of the antibiotic.Ultrastructural studies show...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269636
更新日期:1986-12-01 00:00:00
abstract::Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three d...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233055
更新日期:1993-08-01 00:00:00
abstract::Ethylene emanation rates were assessed from leaf tissues of an embryogenic seed plant (Cycle 0) and regeneration cycle plants selected for enhanced embryogenesis (Cycles I, II and IV). In all experiments, ethylene was assessed from the basal 1 cm portion of the innermost leaf. Ethylene emanation was five-fold higher i...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272059
更新日期:1989-03-01 00:00:00
abstract::KEY MESSAGE : Antioxidant activity of seven leafy vegetables and four beans against five reactive oxygen species and reactive nitrogen species was clearly characterized with a protocol using myoglobin as a reporter probe. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1368-z
更新日期:2013-03-01 00:00:00
abstract::Transgenic papaya (Carica papaya L.) plants were regenerated from embryogenic cultures that were cocultivated with a disarmed C58 strain of Agrobacterium tumefaciens containing one of the following binary cosmid vectors: pGA482GG or pGA482GG/cpPRV-4. The T-DNA region of both binary vectors includes the chimeric genes ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237128
更新日期:1993-03-01 00:00:00
abstract::The transit peptide sequence of ibAGP2 (TP2) was found to be capable of targeting protein into the chloroplast in the Arabidopsis protoplasts. TP2 was fused to a beta-glucuronidase (GUS) reporter gene and expressed in Arabidopsis under the control of the ibAGP2 promoter with the aim of dissecting the effect of the tra...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0563-4
更新日期:2008-08-01 00:00:00
abstract::Shoot cultures of Allium sativum L. were established from stem-tip and scape-tip. Various combinations of media, growth regulators were compared. The best establishment and proliferation of stem-tip and scape-tip explants were obtained on MS medium containing 0.5 mg l(-1) NAA and 0.5 mg l(-1) KT. The roots were produc...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233301
更新日期:1994-11-01 00:00:00
abstract::Quaternary benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelirubine, chelilutine and macarpine) are specifically induced by cell wall components of Penicillium and Saccharomyces in a colorless strain of Eschscholtzia californica cell suspension cultures. Classical elicitors such as the Phytophthora mega...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272770
更新日期:1987-12-01 00:00:00
abstract::Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0695-1
更新日期:2009-06-01 00:00:00
abstract::The expression of green fluorescent protein (GFP) and its inheritance were studied in transgenic oat ( Avena sativa L.) plants transformed with a synthetic green fluorescent protein gene [sgfp(S65T)] driven by a rice actin promoter. In vitro shoot meristematic cultures (SMCs) induced from shoot apices of germinating m...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-002-0542-0
更新日期:2003-01-01 00:00:00
abstract::Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0109-y
更新日期:2006-06-01 00:00:00
abstract::Tissue culture methods for improvement of cotton has lagged seriously compared to other major crops. A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation. Preliminary screening of eight str...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268487
更新日期:1987-06-01 00:00:00
abstract::Callus cultures of Vitis labruscana cv. Concord were initiated from both immature fruit and petiole tissue. Analysis revealed that the acid hydrolyzable precursors to β-damascenone, a compound important to the flavor of most grape species, were present in both callus types. Light and increased sucrose concentration we...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231971
更新日期:1994-05-01 00:00:00
abstract::Transgenic herbicide tolerant Acacia sinuata plants were produced by transformation with the bar gene conferring phosphinothricin resistance. Precultured hypocotyl explants were infected with Agrobacterium tumefaciens strain EHA105 in the presence of 100 microM acetosyringone and shoots regenerated on MS (Murashige an...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0176-8
更新日期:2006-11-01 00:00:00
abstract::Microcallus (more than 60 cells) formation was obtained from leaf mesophyll protoplasts of 6 species and varieties in the genus Actinidia Lindl. (kiwifruit). The best results were achieved by using liquid over agarose culture for A. arguta var. arguta, liquid and agarose disc type culture for A. arguta var. purpurea, ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231583
更新日期:1996-09-01 00:00:00
abstract::Arabidopsis thaliana protoplasts, isolated from a cell suspension, regenerated cell walls and then exhibited sustained cell divisions. Within 10 days after isolation as many as 40% of the protoplast-derived cells divided. Protoplast-derived calli exhibited a wide range of developmental responses, including apparent em...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00820203
更新日期:1990-01-01 00:00:00
abstract::Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269825
更新日期:1986-08-01 00:00:00
abstract::The effects of cytokinins on the different branches of the indole alkaloid pathway were investigated in Catharanthus roseus cell cultures. Addition of zeatin to a 2,4-dichlorophenoxyacetic acid-containing medium decreased tryptamine levels and increased the bioconversion of secologanin to ajmalicine. Zeatin also enhan...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233425
更新日期:1993-10-01 00:00:00
abstract::Carotenoid cleavage dioxygenases (CCDs) are involved in the production of diverse apocarotenoids including phytohormones, the visual molecules and the aromatic volatile compounds derived from carotenoids. Here, we examined the spatial expression of four of the CCD genes (AtCcd1, 4, 7 and 8) among the nine members of t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0999-1
更新日期:2011-06-01 00:00:00
abstract::Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269126
更新日期:1986-06-01 00:00:00
abstract::Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233096
更新日期:1992-10-01 00:00:00
abstract::Callus cultures from cotyledon explants were established and maintained in culture for more than two years. After several months callus cultures were transferred into liquid medium and cultured as cell suspensions. Protoplasts were isolated from these cell suspension cultures and cultured in a liquid medium. After for...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269184
更新日期:1982-06-01 00:00:00
abstract::Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239891
更新日期:1994-01-01 00:00:00
abstract::Extracts of cranberry, rich in flavonols and proanthocyanins, inactivated β-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing the gusA gene. Histochemical GUS assays produced ran...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050294
更新日期:1997-06-01 00:00:00