Shikonin derivative formation on the stem of cultured shoots in Lithospermum erythrorhizon.

Abstract:

:LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography and 1 H nuclear magnetic resonance analyses revealed that the red pigments which accumulated on the cultured shoots were shikonin derivatives. The effects of various basal media and phytohormones (indole-3-acetic acid, indole-3-butyric acid and kinetin) on the growth and the formation of shikonin derivatives were investigated. When the shoots were cultured on Murashige and Skoog solid medium, the addition of kinetin remarkably enhanced shikonin derivative accumulation in the shoots. However, these effects of kinetin were not observed in the liquid culture when cultured in Gamborg B5 medium. The maximum content of shikonin derivatives (2.3% as dry weight, ca. 1.5 mg/100 ml flask) was observed in the shoots cultured in phytohormone-free B5 liquid medium for 5 weeks.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Touno K,Harada K,Yoshimatsu K,Yazaki K,Shimomura K

doi

10.1007/s002990000237

subject

Has Abstract

pub_date

2000-11-01 00:00:00

pages

1121-1126

issue

11

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990000237

journal_volume

19

pub_type

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