Abstract:
:LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography and 1 H nuclear magnetic resonance analyses revealed that the red pigments which accumulated on the cultured shoots were shikonin derivatives. The effects of various basal media and phytohormones (indole-3-acetic acid, indole-3-butyric acid and kinetin) on the growth and the formation of shikonin derivatives were investigated. When the shoots were cultured on Murashige and Skoog solid medium, the addition of kinetin remarkably enhanced shikonin derivative accumulation in the shoots. However, these effects of kinetin were not observed in the liquid culture when cultured in Gamborg B5 medium. The maximum content of shikonin derivatives (2.3% as dry weight, ca. 1.5 mg/100 ml flask) was observed in the shoots cultured in phytohormone-free B5 liquid medium for 5 weeks.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Touno K,Harada K,Yoshimatsu K,Yazaki K,Shimomura Kdoi
10.1007/s002990000237subject
Has Abstractpub_date
2000-11-01 00:00:00pages
1121-1126issue
11eissn
0721-7714issn
1432-203Xpii
10.1007/s002990000237journal_volume
19pub_type
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