Abstract：
:Recently, five novel fluorescent proteins have been isolated from non-bioluminescent species of reef-coral organisms and have been made available through ClonTech. They are AmCyan, AsRed, DsRed, ZsGreen and ZsYellow. These proteins are valuable as reporters for transformation because they do not require a substrate or external co-factor to emit fluorescence and can be tested in vivo without destruction of the tissue under study. We have evaluated them in a large range of plants, both monocots and dicots, and our results indicate that they are valuable reporting tools for transformation in a wide variety of crops. We report here their successful expression in wheat, maize, barley, rice, banana, onion, soybean, cotton, tobacco, potato and tomato. Transient expression could be observed as early as 24 h after DNA delivery in some cases, allowing for very clear visualization of individually transformed cells. Stable transgenic events were generated, using mannose, kanamycin or hygromycin selection. Transgenic plants were phenotypically normal, showing a wide range of fluorescence levels, and were fertile. Expression of AmCyan, ZsGreen and AsRed was visible in maize T1 seeds, allowing visual segregation to more than 99% accuracy. The excitation and emission wavelengths of some of these proteins are significantly different; the difference is enough for the simultaneous visualization of cells transformed with more than one of the fluorescent proteins. These proteins will become useful tools for transformation optimization and other studies. The wide variety of plants successfully tested demonstrates that these proteins will potentially find broad use in plant biology.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Wenck A,Pugieux C,Turner M,Dunn M,Stacy C,Tiozzo A,Dunder E,van Grinsven E,Khan R,Sigareva M,Wang WC,Reed J,Drayton P,Oliver D,Trafford H,Legris G,Rushton H,Tayab S,Launis K,Chang YF,Chen DF,Melchers Ldoi
10.1007/s00299-003-0690-xkeywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
244-51issue
4eissn
0721-7714issn
1432-203Xjournal_volume
22pub_type
杂志文章abstract：:The promotive effect of ethylene inhibitors (Els), i.e. AgNO3 and aminoethoxyvinylglycine (AVG) on de novo shoot regeneration from cultured cotyledonary explants of Brassica campestris ssp. pekinensis cv. Shantung in relation to polyamines (PAs) was investigated. The endogenous levels of free putrescine and spermidine...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232630
更新日期：1994-03-01 00:00:00
abstract：:Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1164-1
更新日期：2012-02-01 00:00:00
abstract：KEY MESSAGE:Rice and chickpea GDPD s are transcriptionally influenced by mineral deficiencies; especially, by phosphate starvation and CaGDP1 encodes an active glycerophosphodiester phosphodiesterase enzyme. Glycerophosphodiester phosphodiesterases (GDPDs) are enzymes involved in the degradation of glycerophosphodieste...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1984-0
更新日期：2016-08-01 00:00:00
abstract：:We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 106 cells/g fresh weight cells and then cultured at a concentration of 2.5×105 cells/ml in NH4NO3-free Mu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050378
更新日期：1998-01-01 00:00:00
abstract：KEY MESSAGE:An ERF gene, LchERF , was cloned from L. chinense for the first time. Overexpression of LchERF conferred salt stress tolerance in transgenic tobacco lines during seed germination and vegetative growth. Ethylene-responsive transcription factors (ERFs) play important roles in tolerance to biotic and abiotic s...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1678-4
更新日期：2014-12-01 00:00:00
abstract：KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1768-y
更新日期：2015-05-01 00:00:00
abstract：:Callus cultures and cell suspension cultures derived from Ginkgo biloba L. leaves produced ginkgolidc B. In cell suspension cultures, the production reached a maximum by the 13th day of subculture and followed by a sharp decrease. The medium of Murashige and Skoog induced the highest ginkgolide B content in cultures w...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232783
更新日期：1995-05-01 00:00:00
abstract：:DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235293
更新日期：1993-12-01 00:00:00
abstract：:Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulat...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232378
更新日期：1992-01-01 00:00:00
abstract：:A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Avera...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0809-8
更新日期：2004-10-01 00:00:00
abstract：:Diarrheal diseases caused by Vibrio cholerae and enterotoxigenic Escherichia coli (ETEC) are worldwide health problems that might be prevented with vaccines based on edible plants expressing the B subunit from either the cholera toxin (CTB) or the E. coli heat labile toxin (LTB). In this work we analyzed the immunity ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0439-z
更新日期：2008-01-01 00:00:00
abstract：KEY MESSAGE:Effective microspore embryogenesis in triticale is determined by a specific hormonal homeostasis: low value of IAA/cytokinins, IAA/ABA and cytokinins/ABA ratios as well as proper endogenous/exogenous auxin balance, which favours androgenic structure formation and green plant regeneration ability. The concen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1686-4
更新日期：2015-01-01 00:00:00
abstract：:Quantitative trait loci (QTLs) associated with androgenic responsiveness in triticale were analyzed using a population of 90 DH lines derived from the F1 cross between inbred line 'Saka 3006' and cv. 'Modus', which was used in a number of earlier studies on molecular mapping in this crop. Using Windows QTL Cartographe...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1320-2
更新日期：2012-11-01 00:00:00
abstract：KEY MESSAGE:The radish WRKY gene family was genome-widely identified and played critical roles in response to multiple abiotic stresses. The WRKY is among the largest transcription factors (TFs) associated with multiple biological activities for plant survival, including control response mechanisms against abiotic stre...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2190-4
更新日期：2017-11-01 00:00:00
abstract：:Programmed cell death (PCD) is a genetically encoded, active process that results in the death of individual cells, tissues, or whole organs, which plays an important role in the life cycles of plants and animals. Previous studies show that methyl salicylate (MeSA) is a defense signal molecular associated with systemi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1031-0
更新日期：2011-07-01 00:00:00
abstract：:Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibite...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232042
更新日期：1991-06-01 00:00:00
abstract：:A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232727
更新日期：1995-07-01 00:00:00
abstract：KEY MESSAGE:Several hypomethylated sites within the Karma region of EgDEF1 and hotspot regions in chromosomes 1, 2, 3, and 5 may be associated with mantling. One of the main challenges faced by the oil palm industry is fruit abnormalities, such as the "mantled" phenotype that can lead to reduced yields. This clonal abn...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02561-9
更新日期：2020-09-01 00:00:00
abstract：:Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0836-5
更新日期：2004-10-01 00:00:00
abstract：KEY MESSAGE:Transcriptome-based gene expression analysis identifies many critical salt-responsive genes in radish and facilitates further dissecting the molecular mechanism underlying salt stress response. Salt stress severely impacts plant growth and development. Radish, a moderately salt-sensitive vegetable crop, has...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1887-5
更新日期：2016-02-01 00:00:00
abstract：:Yucca valida is an important potential source of steroidal saponins closely related to Yucca schidigera, the species that is commercially exploited from the wild as a source of steroidal extracts. Neither of the species has been domesticated mainly because of their slow growth and long life span before harvesting. Her...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0144-3
更新日期：2006-08-01 00:00:00
abstract：:Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232160
更新日期：1992-04-01 00:00:00
abstract：:A NaCl-tolerant callus line of Cicer arietinum has been isolated, as a spontaneous variant, on agar-solidified MS-medium supplemented with 100 mM NaCl. The growth of this line, in the presence of 100 mM NaCl, was comparable to that of the NaCl-sensitive callus line growing in the absence of NaCl. Regarding relative to...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270968
更新日期：1984-04-01 00:00:00
abstract：KEY MESSAGE:Bacterial wilt resistant somatic hybrids were obtained via protoplast fusion between potato and eggplant and three types of nuclear genomes were identified in the hybrids through GISH and SSR analysis. ABSTRACT:Cultivated potato (Solanum tuberosum L.) lacks resistance to bacterial wilt caused by Ralstonia ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1480-8
更新日期：2013-11-01 00:00:00
abstract：:The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed. The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0097-y
更新日期：2006-04-01 00:00:00
abstract：KEY MESSAGE:Transgenic Arabidopsis and lettuce plants overexpressing AtHSP17.8 showed ABA-hypersensitive but abiotic stress-resistant phenotypes. ABA treatment caused a dramatic induction of early ABA-responsive genes in AtHSP17.8 -overexpressing transgenic lettuce. Plant small heat shock proteins function as chaperone...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1506-2
更新日期：2013-12-01 00:00:00
abstract：:Protoplasts isolated enzymatically from seedling roots, hypocotyls and cotyledons of Lotus corniculatus L. produced callus which underwent prolific shoot regeneration. The rapidity and ease of recovering plants from protoplast-derived tissues makes this forage legume an attractive experimental system for genetic manip...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270177
更新日期：1983-04-01 00:00:00
abstract：:The objective of this study was to correlate the time that DNA synthesis first occurs in haploid microspores of barley with cell cycle and plant morphological stages and to subsequently assess the influence of pretreatments on DNA synthesis at different stages of microspore development. Spikes with microspores in earl...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0635-4
更新日期：2003-07-01 00:00:00
abstract：KEY MESSAGE:Transcriptomes of two switchgrass genotypes representing the upland and lowland ecotypes will be key tools in switchgrass genome annotation and biotic and abiotic stress functional genomics. Switchgrass (Panicum virgatum L.) is an important bioenergy feedstock for cellulosic ethanol production. We report ge...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2065-0
更新日期：2017-01-01 00:00:00
abstract：:Independent carrot (Daucus carota) hairy root lines were established by inoculation of discs taken from the same carrot with Agrobacterium rhizogenes 8196 and A. tumefaciens C58C1(pRi8196) carrying pRi8196. Several lines were compared with respect to T-DNA length. One of them was found to have integrated sequences cov...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270112
更新日期：1988-03-01 00:00:00
