Abstract：
:The promotive effect of ethylene inhibitors (Els), i.e. AgNO3 and aminoethoxyvinylglycine (AVG) on de novo shoot regeneration from cultured cotyledonary explants of Brassica campestris ssp. pekinensis cv. Shantung in relation to polyamines (PAs) was investigated. The endogenous levels of free putrescine and spermidine in the explant decreased sharply after 1-3 days of culture, whereas endogenous spermine increased, irrespective of the absence or presence of Els. AgNO3 at 30 μM did not affect endogenous PAs during two weeks of culture. In contrast, explants grown on medium containing 5 μM AVG produced higher levels of free putrescine and spermine which increased rapidly after three days and reached a peak at 10 days. An exogenous application of 5 mM putrescine also resulted in a similar surge of endogenous free spermine of the explant. More strikingly, shoot regeneration from explants grown in the presence of 1-20 mM putrescine, 0.1-2.5 mM spermidine, or 0.1-1 mM spermine was enhanced after three weeks of culture. However, exogenous PAs generally did not affect ethylene production, and endogenous levels of 1-aminocyclopropane-1-carboxylate (ACC) synthase activity and ACC of the explant. This study shows the PA requirement for shoot regeneration from cotyledons of B. campestris ssp. pekinensis in vitro, and also indicates that the promotive effect of PAs on regeneration may not be due to an inhibition of ethylene biosynthesis.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Chi GL,Lin WS,Lee JE,Pua ECdoi
10.1007/BF00232630subject
Has Abstractpub_date
1994-03-01 00:00:00pages
323-9issue
6eissn
0721-7714issn
1432-203Xjournal_volume
13pub_type
杂志文章abstract：:A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231799
更新日期：1991-03-01 00:00:00
abstract：KEY MESSAGE:An antigenic protein targeting two epitopes from the Zaire ebolavirus GP1 protein was expressed in plant cells rendering an antigen capable of inducing humoral responses in mouse when administered subcutaneously or orally. The 2014 Ebola outbreak made clear that new treatments and prophylactic strategies to...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2088-6
更新日期：2017-02-01 00:00:00
abstract：:The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenou...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0046-9
更新日期：2006-03-01 00:00:00
abstract：:Photosynthetically active callus and cell suspension cultures were established from uninfected Lycopersicon peruvianum plants and from uninfected and potato spindle tuber viroid (PSTVd) infected plants of Lycopersicon esculentum cv. Rutgers. Viroid infection was maintained in photoheterotrophic culture on media contai...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232806
更新日期：1993-09-01 00:00:00
abstract：:The goal of the study was to characterize the optimal developmental stage of zygotic embryo expiants of the hardwood forest tree species yellow-poplar (Liriodendron tulipifera L.) for the initiation of embryogenic cultures, using morphological measurements and polypeptide profiles of the embryos. Developing zygotic em...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234289
更新日期：1991-07-01 00:00:00
abstract：:Low initiation frequency is one of the main barriers in applying somatic embryogenesis to the clonal production of Pinus species. Factors affecting initiation, including basal medium, plant growth regulators, and Phytagel concentration, have been investigated in loblolly pine (Pinus taeda L.). BM1 basal medium proved ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050396
更新日期：1998-02-01 00:00:00
abstract：: Embryogenic nucellar callus cultures of different Citrus species and cultivars growing in hormone-free medium were transferred to medium containing either sucrose or glycerol as the only carbohydrate source. Glycerol has been reported to induce further development of Citrus somatic embryos, while in the presence of s...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000280
更新日期：2001-01-01 00:00:00
abstract：KEY MESSAGE:This study established an efficient method of regenerating plants of Ficus lyrata and producing purple-leaved F. lyrata plants through genetic transformation using a VvMybA1 gene of grapevine. ABSTRACT:Ficus lyrata, a species with unique violin- or guitar-shaped leaves, was regenerated from leaf-derived ca...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1491-5
更新日期：2013-11-01 00:00:00
abstract：:Brassica juncea L. is a Zn/Cd accumulator. To determine the physiological basis of its metal accumulation phenotype, the functional properties and role of the metal efflux transporter BjCET2 were investigated using transgenic technology. Heterologous expression of BjCET2 in the double mutant yeast strain Deltazrc1Delt...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0723-1
更新日期：2009-08-01 00:00:00
abstract：:Programmed cell death (PCD) is a process that occurs both in animals and in plants and is an essential element in developmental processes. Pollination is a key factor in fruit production and self-incompatibility is one of the main limiting factors of this process. PCD has recently been put forward as a possible cause ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0845-5
更新日期：2010-06-01 00:00:00
abstract：:Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232092
更新日期：1990-07-01 00:00:00
abstract：KEY MESSAGE:The cytokinin pathway promotes the initiation of bulbil formation, and iPA may an important type of cytokinin during bulbil formation in Lilium lancifolium. Bulbils are important vegetative reproductive organs in triploid Lilium lancifolium. We previously showed that cytokinins are involved in bulbil format...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02535-x
更新日期：2020-07-01 00:00:00
abstract：:In vitro clonal propagation of plants should generate identical copies of the selected genotype. However, associated stress might result in a breakdown of control mechanisms and consequent instability of the genome. We have used several molecular methods to assess the genetic stability of long-term propagated (24 year...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0413-9
更新日期：2007-11-01 00:00:00
abstract：:Axillary buds from 5 genotypes of mulberry belonging to 4 species were cultured on modified MS basal medium. A total of 30 media combinations were tried for all the genotypes. The response of axillary buds and the requirement for growth regulators varied with genotype. In Morus indica BAP (0.25-0.5 mg/l), and in M. al...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272107
更新日期：1990-05-01 00:00:00
abstract：:Vitrification is a technically simple method for cryopreserving plant germplasm, requiring only the application of suitable cryoprotectants and rapid cooling rates. Sweetpotato (Ipomoea batatas [L.] Lam.) shoot tips obtained from in vitro plants survived liquid nitrogen (-196°C) exposure following a vitrification-indu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232527
更新日期：1992-05-01 00:00:00
abstract：KEY MESSAGE:Oral administration of maize-expressed H3N2 nucleoprotein induced antibody responses in mice showing the immunogenicity of plant-derived antigen and its potential to be utilized as a universal flu vaccine. Influenza A viruses cause influenza epidemics that are devastating to humans and livestock. The vaccin...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1758-0
更新日期：2015-06-01 00:00:00
abstract：:Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232275
更新日期：1990-12-01 00:00:00
abstract：:The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0500-y
更新日期：2008-05-01 00:00:00
abstract：KEY MESSAGE:A complete set of monosomic alien addition lines of Brassica napus with one of the seven chromosomes of Isatis indigotica and the recombinant mitochondria was developed and characterized. Monosomic alien addition lines (MAALs) are valuable for elucidating the genome structure and transferring the useful gen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1621-8
更新日期：2014-08-01 00:00:00
abstract：:Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and alpha-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0224-4
更新日期：2007-02-01 00:00:00
abstract：:Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on l...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0080-7
更新日期：2006-05-01 00:00:00
abstract：:Microcallus (more than 60 cells) formation was obtained from leaf mesophyll protoplasts of 6 species and varieties in the genus Actinidia Lindl. (kiwifruit). The best results were achieved by using liquid over agarose culture for A. arguta var. arguta, liquid and agarose disc type culture for A. arguta var. purpurea, ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231583
更新日期：1996-09-01 00:00:00
abstract：KEY MESSAGE:Overexpression of Withania somnifera SGT gene (WssgtL3.1) in transgenic Arabidopsis improves various agronomic and physiological traits and alters conjugated sterol levels to mitigate the effect of salt stress. Sterols are essential constituents of cell membranes that are involved in several biological func...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-021-02666-9
更新日期：2021-02-01 00:00:00
abstract：:The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as thos...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s002990100346
更新日期：2001-07-01 00:00:00
abstract：:Cell walls and media were obtained from three kinds of carrot cell culture, namely, embryogenic callus (EC), non-embryogenic callus (NC) and somatic embryos (SE), and analyzed for their sugar content and sugar composition by electrophoresis and gas chromatography. EC formed large cell clusters while NC formed small cl...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232028
更新日期：1995-02-01 00:00:00
abstract：:Methods of shoot and plant development from cotyledon explants of the tomato cultivar 'Bonner Beste' and its mutant chloronerva are described. Cotyledon mesophyll protoplasts of the mutant chloronerva have been isolated and cultured, and induced to regenerate shoots and plants. By grafting regenerated shoots of the ch...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270102
更新日期：1983-08-01 00:00:00
abstract：:Diuraphis noxia (Russian wheat aphid, RWA) is a major pest on wheat in South Africa and most other wheat growing countries. Being a probing-sucking insect, RWAs insert their stylets into the phloem sieve elements and feed on the phloem sap. This feeding causes necrotic lesions in resistant varieties, or decoloration o...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0001-9
更新日期：2006-02-01 00:00:00
abstract：:Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232295
更新日期：1990-09-01 00:00:00
abstract：:Expression of the green fluorescent protein (gfp) gene, under regulatory control of either the constitutive 35S promoter or the developmentally-regulated lectin promoter was monitored and quantified using a newly-developed automated tracking system. The automated system consisted of a computer-controlled two-dimension...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0142-5
更新日期：2006-09-01 00:00:00
abstract：:Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232160
更新日期：1992-04-01 00:00:00
