Abstract:
KEY MESSAGE:The cytokinin pathway promotes the initiation of bulbil formation, and iPA may an important type of cytokinin during bulbil formation in Lilium lancifolium. Bulbils are important vegetative reproductive organs in triploid Lilium lancifolium. We previously showed that cytokinins are involved in bulbil formation, but how cytokinins participate in bulbil formation is not clear. In this study, bulbil formation was divided into three stages on the basis of anatomical and histological observations: the bulbil initiation stage, bulbil primordium-formation stage and bulbil structure-formation stage. The results indicated that iPA was the most critical cytokinin during the bulbil initiation. qRT-PCR revealed that increased iPA content during bulbil initiation was mainly due to increased expression of cytokinin synthesis genes (IPT1/5) and cytokinin activation genes (LOG1/3/5/7) and significantly decreased expression of the cytokinin degradation gene CKX4. Exogenous 6-BA and lovastatin affected the cytokinin pathway and promoted or inhibited bulbil initiation by increasing or decreasing the content of endogenous iPA, respectively. In summary, we demonstrate that cytokinins positively regulate bulbil formation and provide preliminary insight into the regulatory mechanisms by which the cytokinin pathway promotes bulbil initiation.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
He G,Yang P,Tang Y,Cao Y,Qi X,Xu L,Ming Jdoi
10.1007/s00299-020-02535-xsubject
Has Abstractpub_date
2020-07-01 00:00:00pages
861-872issue
7eissn
0721-7714issn
1432-203Xpii
10.1007/s00299-020-02535-xjournal_volume
39pub_type
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journal_title:Plant cell reports
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journal_title:Plant cell reports
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050501
更新日期:1998-08-01 00:00:00