Mechanism of exogenous cytokinins inducing bulbil formation in Lilium lancifolium in vitro.

Abstract:

KEY MESSAGE:The cytokinin pathway promotes the initiation of bulbil formation, and iPA may an important type of cytokinin during bulbil formation in Lilium lancifolium. Bulbils are important vegetative reproductive organs in triploid Lilium lancifolium. We previously showed that cytokinins are involved in bulbil formation, but how cytokinins participate in bulbil formation is not clear. In this study, bulbil formation was divided into three stages on the basis of anatomical and histological observations: the bulbil initiation stage, bulbil primordium-formation stage and bulbil structure-formation stage. The results indicated that iPA was the most critical cytokinin during the bulbil initiation. qRT-PCR revealed that increased iPA content during bulbil initiation was mainly due to increased expression of cytokinin synthesis genes (IPT1/5) and cytokinin activation genes (LOG1/3/5/7) and significantly decreased expression of the cytokinin degradation gene CKX4. Exogenous 6-BA and lovastatin affected the cytokinin pathway and promoted or inhibited bulbil initiation by increasing or decreasing the content of endogenous iPA, respectively. In summary, we demonstrate that cytokinins positively regulate bulbil formation and provide preliminary insight into the regulatory mechanisms by which the cytokinin pathway promotes bulbil initiation.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

He G,Yang P,Tang Y,Cao Y,Qi X,Xu L,Ming J

doi

10.1007/s00299-020-02535-x

subject

Has Abstract

pub_date

2020-07-01 00:00:00

pages

861-872

issue

7

eissn

0721-7714

issn

1432-203X

pii

10.1007/s00299-020-02535-x

journal_volume

39

pub_type

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