Abstract:
:Transgenic tobacco plants expressing an inhibitor of ubiquitin-dependent protein degradation, ubiquitin variant ubR48, spontaneously formed necrotic lesions and displayed altered responsiveness to tobacco mosaic virus attack. These plants were analyzed for the accumulation of defense-related compounds and the expression of pathogenesis-related proteins, which serve as convenient markers for systemic acquired resistance. Callose was detected in the cells of vascular bundles and in the leaf blade. In addition, ubR48 transgenic plants constitutively accumulated enhanced levels of salicylic acid (SA) and/or its glucoside. Accumulation of SA glucoside coincided with high levels of pathogenesis-related protein 1, underscoring the similarity of certain changes in ubR48-expressing plants to an authentic defense reaction.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Conrath U,Klessig DF,Bachmair Adoi
10.1007/s002990050501subject
Has Abstractpub_date
1998-08-01 00:00:00pages
876-880issue
11eissn
0721-7714issn
1432-203Xpii
10.1007/s002990050501journal_volume
17pub_type
杂志文章abstract::Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootsto...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0422-8
更新日期:2008-01-01 00:00:00
abstract::A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0697-z
更新日期:2009-06-01 00:00:00
abstract::The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0920-5
更新日期:2005-03-01 00:00:00
abstract:KEY MESSAGE:We report global gene expression patterns of poplar in response to chilling stress. A total of 1,085 significantly differentially expressed genes, involved in photosynthesis, signal transduction, and regulation of transcription, were identified. To understand the gene network underlying the response to chil...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1454-x
更新日期:2013-09-01 00:00:00
abstract::An efficient plant regeneration system from leaf-derived callus in 6 genotypes of Avena sativa L. has been established. Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. Plants were regenerated from the regenerable callus and grown to maturity. The frequency of regenerable callus f...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00238604
更新日期:1995-03-01 00:00:00
abstract::A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 μM 2,4-D or NAA in combination with 10 μM BA, or 25 μM 2,4-D alone. The callus grew most vigorously o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232946
更新日期:1994-06-01 00:00:00
abstract::Cell walls and media were obtained from three kinds of carrot cell culture, namely, embryogenic callus (EC), non-embryogenic callus (NC) and somatic embryos (SE), and analyzed for their sugar content and sugar composition by electrophoresis and gas chromatography. EC formed large cell clusters while NC formed small cl...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232028
更新日期:1995-02-01 00:00:00
abstract::Accurate and timely detection of transgene copy number in sugarcane is currently hampered by the requirement to use Southern blotting, needing relatively large amounts of genomic DNA and, therefore, the continued growth and maintenance of bulky plants in containment glasshouses. In addition, the sugarcane genome is bo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1150-7
更新日期:2012-01-01 00:00:00
abstract::Shoot regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L.) was tested on MS basal medium supplemented with four different growth regulators. Regeneration frequencies for medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 60 μM 4amino-3, 5,6-picolinic acid (piclora...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232655
更新日期:1995-08-01 00:00:00
abstract::Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus inductio...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01690249
更新日期:1995-01-01 00:00:00
abstract::Root vigor is an important trait for the growth of terrestrial plants, especially in water-deficit environments. Although deserts plants are known for their highly developed root architecture, the molecular mechanism responsible for this trait has not been determined. Here we established an efficient protocol for the ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0863-3
更新日期:2010-07-01 00:00:00
abstract:KEY MESSAGE:A R2R3-MYB transcription factor EsAN2 was isolated from Epimedium sagittatum and functionally characterized to regulate the anthocyanin biosynthetic pathway. Epimedium plants are used widely both as traditional Chinese medicinal herbs and ornamental perennials. Anthocyanins, acting as major contributors to ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1929-z
更新日期:2016-04-01 00:00:00
abstract::Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. 'Russet Burbank'. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233028
更新日期:1991-05-01 00:00:00
abstract::Taro (Colocasia esculenta) is one of the most important crops in the Pacific Islands, however, taro yields have been declining in Hawaii over the past 30 years partly due to diseases caused by oomycete and fungal pathogens. In this study, an efficient Agrobacterium tumefaciens-mediated transformation method for taro i...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0519-8
更新日期:2008-05-01 00:00:00
abstract::Mimosa tenuiflora (Willd.) Poiret (Leguminosae) was micropropagated throughin vitro culture of axillary buds on Murashige and Skoog (MS) medium. Shoot formation was achieved when the media were supplemented with 0.1 mg.L(-1) IAA + 3 mg.L(-1) KN.In vitro rooting of regenerated shoots was achieved when 0.1 mg.L(-1) KN w...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275455
更新日期:1996-11-01 00:00:00
abstract::Kidney vetch seedlings were induced to form hairy roots by inoculating their mesocotyls with the wild-type strain 15834 of Agrobacterium rhizogenes or with the A. tumefaciens strain C58C1 containing a binary vector system (the pRiA4b as a helper and the vector pCB1346 bearing a pTiC58-derived isopentenyl transferase g...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233367
更新日期:1992-07-01 00:00:00
abstract::The transit peptide sequence of ibAGP2 (TP2) was found to be capable of targeting protein into the chloroplast in the Arabidopsis protoplasts. TP2 was fused to a beta-glucuronidase (GUS) reporter gene and expressed in Arabidopsis under the control of the ibAGP2 promoter with the aim of dissecting the effect of the tra...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0563-4
更新日期:2008-08-01 00:00:00
abstract::RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV)...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0911-z
更新日期:2010-11-01 00:00:00
abstract::This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that wer...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0687-5
更新日期:2003-11-01 00:00:00
abstract:KEY MESSAGE:Red alga, Gracilariopsis chorda, contains seven carbonic anhydrases that can be grouped into α-, β- and γ-classes. Carbonic anhydrases (CAHs) are metalloenzymes that catalyze the reversible hydration of CO2. These enzymes are present in all living organisms and play roles in various cellular processes, incl...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-018-2356-8
更新日期:2019-02-01 00:00:00
abstract::Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobact...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0036-y
更新日期:2006-03-01 00:00:00
abstract::Microcallus (more than 60 cells) formation was obtained from leaf mesophyll protoplasts of 6 species and varieties in the genus Actinidia Lindl. (kiwifruit). The best results were achieved by using liquid over agarose culture for A. arguta var. arguta, liquid and agarose disc type culture for A. arguta var. purpurea, ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231583
更新日期:1996-09-01 00:00:00
abstract::A procedure has been developed for the clonal propagation of Picrorhiza kurroa Royle ex Benth. through shoot tip culture. Murashige and Skoog's medium (1962) supplemented with kinetin (3.0 to 5.0 mg/l) supported rapid proliferation of multiple shoots from the explants. Addition of indole-3-acetic acid (1.0 mg/l) to th...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269324
更新日期:1988-05-01 00:00:00
abstract::Embryo development and germination of Cyclamen persicum have been comparatively characterized for zygotic and somatic embryos with regard to mitotic activity and morphology in order to identify developmental abnormalities in somatic embryogenesis. Zygotic embryo development proved to be highly synchronous with distinc...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0130-9
更新日期:2006-07-01 00:00:00
abstract::A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232265
更新日期:1990-12-01 00:00:00
abstract:KEY MESSAGE:The zeaxanthin epoxidase gene ( MsZEP ) was cloned and characterized from alfalfa and validated for its function of tolerance toward drought and salt stresses by heterologous expression in Nicotiana tabacum. Zeaxanthin epoxidase (ZEP) plays important roles in plant response to various environment stresses d...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1895-5
更新日期:2016-02-01 00:00:00
abstract::The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01890855
更新日期:1996-12-01 00:00:00
abstract::A procedure to produce fruits from cultured shoot tips of Cucumis sativus L. cultivar 'Marketmore-76' in vitro is described. Four-week-old shoot tips, derived from sterile germinated seedlings on a MS medium, were cultured in a 3.8-1 Mason jar using an automated plant culture system. Tips readily generated roots, leav...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232312
更新日期:1993-11-01 00:00:00
abstract::The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro technique...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1829-2
更新日期:2015-10-01 00:00:00
abstract::Sweet cherry shows S-RNase-based gametophytic self-incompatibility, which prevents self- and cross-fertilization between genetically related individuals. The specificity of the self-incompatible reaction is determined by two genes located in the S-locus. These encode a pistil-expressed ribonuclease (S-RNase) that inhi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0857-1
更新日期:2010-07-01 00:00:00