Abstract：
KEY MESSAGE:We developed transgenic sweet potato with Spomin (sucrose-inducible minimal promoter)-GUS gene-fused constructs. Induced GUS activities by Spomin were higher than those by CaMV 35S promoter. We developed transgenic sweet potato (Ipomoea batatas L. Lam. cv. Kokei no. 14) plants with Spomin (sucrose-inducible minimal promoter)-GUS gene-fused constructs with signal peptides for sorting to cytosol, apoplast and ER, and we analyzed the GUS expression pattern of cut tissue after sucrose treatment. Induced GUS activities by Spomin were several hundred times higher than those by the CaMV 35S promoter. Also, GUS activities in storage roots induced with a Spomin-cytosol-GUS construct were higher than those with either Spomin-apoplast or -ER-GUS constructs. The induced GUS activities by Spomin were higher in storage roots without sucrose treatment than those with sucrose treatment. Chilling (4 °C) storage roots with Spomin constructs for 4 weeks produced higher GUS activities than in storage roots stored at 25 °C for 4 weeks. The calculated maximum GUS content in the storage roots was up to about 224.2 μg/g fresh weight. The chilling treatment increased the free sucrose content in the storage roots, and this increase in endogenous sugar levels induced increased GUS activities in the storage roots. Therefore, Spomin appears to be a useful promoter to develop protein production systems using sweet potato variety Kokei no. 14 storage roots by postharvest treatment.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Honma Y,Yamakawa Tdoi
10.1007/s00299-019-02453-7subject
Has Abstractpub_date
2019-11-01 00:00:00pages
1417-1426issue
11eissn
0721-7714issn
1432-203Xpii
10.1007/s00299-019-02453-7journal_volume
38pub_type
杂志文章abstract：:Recently, five novel fluorescent proteins have been isolated from non-bioluminescent species of reef-coral organisms and have been made available through ClonTech. They are AmCyan, AsRed, DsRed, ZsGreen and ZsYellow. These proteins are valuable as reporters for transformation because they do not require a substrate or...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0690-x
更新日期：2003-11-01 00:00:00
abstract：KEY MESSAGE:Our study shows that the expression of AtCBF3 and AtCOR15A improved the chilling tolerance in transgenic eggplant. In an attempt to improve chilling tolerance of eggplant (Solanum melongena L) plants, Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) genes both driven by an Ar...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1670-z
更新日期：2014-12-01 00:00:00
abstract：:Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(me...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0621-x
更新日期：2003-08-01 00:00:00
abstract：:A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234146
更新日期：1994-04-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00820203
更新日期：1990-01-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02409-x
更新日期：2019-07-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2228-7
更新日期：2018-02-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0682-x
更新日期：2003-11-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0439-z
更新日期：2008-01-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02482-2
更新日期：2020-01-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232455
更新日期：1996-04-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00193714
更新日期：1995-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000198
更新日期：2000-09-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233152
更新日期：1996-08-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0095-0
更新日期：2006-06-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1773-1
更新日期：2015-07-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232330
更新日期：1991-02-01 00:00:00
abstract：:Green fluorescent protein (GFP) has been used widely as a powerful bioluminescent reporter, but its visualization by existing methods in tissues or whole plants and its utilization for high-throughput screening remains challenging in many species. Here, we report a fluorescence image analyzer-based method for GFP dete...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0657-z
更新日期：2009-03-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231624
更新日期：1994-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00299-009-0712-4
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050004
更新日期：2000-01-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0974-x
更新日期：2011-04-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272039
更新日期：1989-03-01 00:00:00
abstract：:Somatic hybrids between Solanum tuberosum cv 'Brodick' and S. sanctae-rosae were selected for having elevated chromosome numbers, intermediate morphology and potato cyst nematode resistance (derived from S. sanctae-rosae). DNA was extracted from this material and subjected to inter-SSR PCR directed by oligonucleotide ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050735
更新日期：1999-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232412
更新日期：1992-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1254-8
更新日期：2012-08-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-017-2127-y
更新日期：2017-08-01 00:00:00
abstract：:A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 μM 2,4-D or NAA in combination with 10 μM BA, or 25 μM 2,4-D alone. The callus grew most vigorously o...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232946
更新日期：1994-06-01 00:00:00
abstract：KEY MESSAGE:Rice and chickpea GDPD s are transcriptionally influenced by mineral deficiencies; especially, by phosphate starvation and CaGDP1 encodes an active glycerophosphodiester phosphodiesterase enzyme. Glycerophosphodiester phosphodiesterases (GDPDs) are enzymes involved in the degradation of glycerophosphodieste...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1984-0
更新日期：2016-08-01 00:00:00
