Production of a hepatoprotective cerebroside from suspension cultures of Lycium chinense.

abstract：:A reliable and efficient system for transformation and regeneration of 'Chardonnay' (Vitis vinifera L.) plants via microprojectile bombardment was developed. Improvements over the previous biolistic transformation system included: (1) the use of gold particles for bombardment; (2) step-wise selection at 10 then 15 mg/...

journal_title：Plant cell reports

authors： Vidal JR,Kikkert JR,Wallace PG,Reisch BI

更新日期：2003-11-01 00:00:00

abstract：:Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparati...

journal_title：Plant cell reports

authors： Li LC,Kohlenbach HW

更新日期：1982-10-01 00:00:00

abstract：KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...

journal_title：Plant cell reports

authors： Machado RD,Christoff AP,Loss-Morais G,Margis-Pinheiro M,Margis R,Körbes AP

更新日期：2015-07-01 00:00:00

abstract：KEY MESSAGE:The recovery of non-functional-enhanced green fluorescence protein can be used as indicator to facilitate the identification of mutants generated by CRISPR/Cas9. The CRISPR/Cas9 system is a powerful tool for genome editing and it has been employed to knock out genes of interest in multiple plant species. Id...

journal_title：Plant cell reports

authors： Ren C,Guo Y,Gathunga EK,Duan W,Li S,Liang Z

更新日期：2019-12-01 00:00:00

abstract：:Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulat...

journal_title：Plant cell reports

authors： Pillai KG,Rao IU,Rao IV,Ram HY

更新日期：1992-01-01 00:00:00

abstract：KEY MESSAGE:We describe a steroid-inducible BABY BOOM system that improves plant regeneration in Arabidopsis leaf cultures and yields fertile plants. Regeneration of Arabidopsis thaliana plants for extended periods of time in tissue culture may result in sterile plants. We report here a novel approach for A. thaliana r...

journal_title：Plant cell reports

authors： Lutz KA,Martin C,Khairzada S,Maliga P

更新日期：2015-10-01 00:00:00

abstract：:Taro (Colocasia esculenta) is one of the most important crops in the Pacific Islands, however, taro yields have been declining in Hawaii over the past 30 years partly due to diseases caused by oomycete and fungal pathogens. In this study, an efficient Agrobacterium tumefaciens-mediated transformation method for taro i...

journal_title：Plant cell reports

authors： He X,Miyasaka SC,Fitch MM,Moore PH,Zhu YJ

更新日期：2008-05-01 00:00:00

abstract：KEY MESSAGE:We report global gene expression patterns of poplar in response to chilling stress. A total of 1,085 significantly differentially expressed genes, involved in photosynthesis, signal transduction, and regulation of transcription, were identified. To understand the gene network underlying the response to chil...

journal_title：Plant cell reports

authors： Song Y,Chen Q,Ci D,Zhang D

更新日期：2013-09-01 00:00:00

abstract：:Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 microM of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that...

journal_title：Plant cell reports

authors： Yoshihara T,Hodoshima H,Miyano Y,Shoji K,Shimada H,Goto F

更新日期：2006-04-01 00:00:00

abstract：:The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...

journal_title：Plant cell reports

authors： Tian L,Séguin A,Charest PJ

更新日期：1997-02-01 00:00:00

abstract：:Surface-sterilized leaf disks of horse-radish (Armoracia lapathifolia) were immersed in a suspension of Agrobacterium rhizogenes harboring the root-inducing plasmid (pRi) and cultured on a solid medium. Within about 10 days after inoculation, adventitious roots (hairy roots) emerged from the leaf disks. No roots emerg...

journal_title：Plant cell reports

authors： Noda T,Tanaka N,Mano Y,Nabeshima S,Ohkawa H,Matsui C

更新日期：1987-07-01 00:00:00

abstract：:Shoot cultures of Allium sativum L. were established from stem-tip and scape-tip. Various combinations of media, growth regulators were compared. The best establishment and proliferation of stem-tip and scape-tip explants were obtained on MS medium containing 0.5 mg l(-1) NAA and 0.5 mg l(-1) KT. The roots were produc...

journal_title：Plant cell reports

authors： Ma Y,Wang HL,Zhang CJ,Kang YQ

更新日期：1994-11-01 00:00:00

abstract：:We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were ...

journal_title：Plant cell reports

authors： Renau-Morata B,Arrillaga I,Segura J

更新日期：2006-07-01 00:00:00

abstract：: An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. T...

journal_title：Plant cell reports

authors： Eady CC,Weld RJ,Lister CE

更新日期：2000-03-01 00:00:00

abstract：:The lateral cotyledonary meristems of germinatingPisum sativum cv. Puget seeds were used to develop a reproducibleAgrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerab...

journal_title：Plant cell reports

authors： Bean SJ,Gooding PS,Mullincaux PM,Davies DR

更新日期：1997-05-01 00:00:00

abstract：:Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on l...

journal_title：Plant cell reports

authors： Wang T,Ran Y,Atkinson RG,Gleave AP,Cohen D

更新日期：2006-05-01 00:00:00

abstract：:An early identification of fusion products was based on the presumed vigorous growth of hybrid calluses after fusion between Solanum brevidens and S. tuberosum leaf protoplasts. The S. brevidens protoplasts were unable to form multicellular colonies under the applied culture conditions. Three size groups of calluses w...

journal_title：Plant cell reports

authors： Polgár Z,Preiszner J,Dudits D,Fehér A

更新日期：1993-05-01 00:00:00

abstract：:The anthracnose fungus Colletotrichum gloeosporioides deleteriously affects unripe pepper fruit, but not ripe fruit. Here, we show that the induction of local acquired resistance (LAR) by salicylic acid (SA), 2,6-dichloroisonicotinic acid, or benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester pretreatment pro...

journal_title：Plant cell reports

authors： Lee S,Hong JC,Jeon WB,Chung YS,Sung S,Choi D,Joung YH,Oh BJ

更新日期：2009-10-01 00:00:00

abstract：:Advancement in the field of genetics and genomics after the discovery of Mendel's laws of inheritance has led to map the genes controlling qualitative and quantitative traits in crop plant species. Mapping of genomic regions controlling the variation of quantitatively inherited traits has become routine after the adve...

journal_title：Plant cell reports

authors： Kumar J,Gupta DS,Gupta S,Dubey S,Gupta P,Kumar S

更新日期：2017-08-01 00:00:00

abstract：:The efficacy of benzyladenine (BA) to induce multiple shoots from seed explants of chickpea (Cicer arietinum L.) was assessed. Shoot differentiation was influenced by the type of seed explant, genotype and concentration of BA. Orientation of the explant also strongly influenced the shoot regeneration response. The opt...

journal_title：Plant cell reports

authors： Polisetty R,Paul V,Deveshwar JJ,Khetarpal S,Suresh K,Chandra R

更新日期：1997-05-01 00:00:00

abstract：:The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...

journal_title：Plant cell reports

authors： Liu Y,Lou Q,Xu W,Xin Y,Bassett C,Wang Y

更新日期：2011-12-01 00:00:00

abstract：:A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...

journal_title：Plant cell reports

authors： Cannon G,Heinhorst S,Siedlecki J,Weissbach A

更新日期：1985-04-01 00:00:00

abstract：:A test system for root regeneration was developed that consists of stem slices of apple shoots (ca. 0.5 mm thick; fresh weight ca. 1 mg). Roots regenerated synchronously without intervening callus formation and without interference with compounds originating from other parts of the plant. Supply of indolebutyric acid ...

journal_title：Plant cell reports

authors： Van der Krieken WM,Breteler H,Visser MH,Mavridou D

更新日期：1993-02-01 00:00:00

abstract：KEY MESSAGE:We developed a method for detection of mutants in a large number of plants, and found this method to be applicable to detection of a mutant allele at a concentration of 1/1000. Many techniques for SNP analysis have been developed, but most of these techniques are not so sensitive to be used for detection of...

journal_title：Plant cell reports

authors： Kohata R,Koitabashi K,Kitashiba H,Nishio T

更新日期：2018-06-01 00:00:00

abstract：:Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes. The transformed cultures of ginseng could be subcultured as an axenic root culture in the absence of phytohormones, and grew with extensive lateral branches more rapidly t...

journal_title：Plant cell reports

authors： Yoshikawa T,Furuya T

更新日期：1987-12-01 00:00:00

abstract：:Methods of shoot and plant development from cotyledon explants of the tomato cultivar 'Bonner Beste' and its mutant chloronerva are described. Cotyledon mesophyll protoplasts of the mutant chloronerva have been isolated and cultured, and induced to regenerate shoots and plants. By grafting regenerated shoots of the ch...

journal_title：Plant cell reports

authors： Koblitz H,Koblitz D

更新日期：1983-08-01 00:00:00

abstract：KEY MESSAGE:Transcriptome analysis of barley embryogenic callus from isolated microspore culture under salt stress uncovered a role of translation inhibition and selective activation of stress-specific proteins in cellular defense. Soil salinity is one of the major abiotic stresses which constrains the plant growth and...

journal_title：Plant cell reports

authors： Liu CH,Lu RJ,Guo GM,He T,Li YB,Xu HW,Gao RH,Chen ZW,Huang JH

更新日期：2016-08-01 00:00:00

abstract：:The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...

journal_title：Plant cell reports

authors： Sethi U,Basu A,Mukherjee SG

更新日期：1990-03-01 00:00:00

abstract：KEY MESSAGE:Oral administration of maize-expressed H3N2 nucleoprotein induced antibody responses in mice showing the immunogenicity of plant-derived antigen and its potential to be utilized as a universal flu vaccine. Influenza A viruses cause influenza epidemics that are devastating to humans and livestock. The vaccin...

journal_title：Plant cell reports

authors： Nahampun HN,Bosworth B,Cunnick J,Mogler M,Wang K

更新日期：2015-06-01 00:00:00

abstract：:Somatic hybrids between Solanum tuberosum cv 'Brodick' and S. sanctae-rosae were selected for having elevated chromosome numbers, intermediate morphology and potato cyst nematode resistance (derived from S. sanctae-rosae). DNA was extracted from this material and subjected to inter-SSR PCR directed by oligonucleotide ...

journal_title：Plant cell reports

authors： Matthews D,McNicoll J,Harding K,Millam S

更新日期：1999-12-01 00:00:00