Abstract:
:The lateral cotyledonary meristems of germinatingPisum sativum cv. Puget seeds were used to develop a reproducibleAgrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal, self-fertile plants containing functional transgenes inherited in a Mendelian fashion.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Bean SJ,Gooding PS,Mullincaux PM,Davies DRdoi
10.1007/BF01142315subject
Has Abstractpub_date
1997-05-01 00:00:00pages
513-519issue
8eissn
0721-7714issn
1432-203Xpii
10.1007/BF01142315journal_volume
16pub_type
杂志文章abstract::Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three d...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233055
更新日期:1993-08-01 00:00:00
abstract::Expression of the green fluorescent protein (gfp) gene, under regulatory control of either the constitutive 35S promoter or the developmentally-regulated lectin promoter was monitored and quantified using a newly-developed automated tracking system. The automated system consisted of a computer-controlled two-dimension...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0142-5
更新日期:2006-09-01 00:00:00
abstract::Callus cultures of Vitis labruscana cv. Concord were initiated from both immature fruit and petiole tissue. Analysis revealed that the acid hydrolyzable precursors to β-damascenone, a compound important to the flavor of most grape species, were present in both callus types. Light and increased sucrose concentration we...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231971
更新日期:1994-05-01 00:00:00
abstract:KEY MESSAGE:We developed transgenic sweet potato with Spomin (sucrose-inducible minimal promoter)-GUS gene-fused constructs. Induced GUS activities by Spomin were higher than those by CaMV 35S promoter. We developed transgenic sweet potato (Ipomoea batatas L. Lam. cv. Kokei no. 14) plants with Spomin (sucrose-inducible...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02453-7
更新日期:2019-11-01 00:00:00
abstract:KEY MESSAGE:An RNAseq-based analysis of the cassava plants inoculated with Xam allowed the identification of transcriptional upregulation of genes involved in jasmonate metabolism, phenylpropanoid biosynthesis and putative targets for a TALE. Cassava bacterial blight, a disease caused by the gram-negative bacterium Xan...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1667-7
更新日期:2014-11-01 00:00:00
abstract:KEY MESSAGE:By comparing series full-length cDNA libraries stressed and control, the dynamic process of salt stress response in Upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed. The Upland cotton is the most important fiber plant with highly salt tolerance. Howeve...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1761-5
更新日期:2015-06-01 00:00:00
abstract::In an attempt to improve growth and secondary metabolite production, and to understand the possible mechanism involved in relation to the changes in physiology and activities of antioxidant enzymes, we cultured Morinda citrifolia adventitious roots in different strength (0.25, 0.50, 0.75, 1.0, 1.5 and 2.0) of Murashig...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0854-4
更新日期:2010-07-01 00:00:00
abstract::Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193724
更新日期:1995-12-01 00:00:00
abstract::Essential oils were produced by flowers of Hyacinthus orientalis L. that had been regenerated in vitro. The production of these oils was affected by the concentration of gibberellic acid and sucrose in the medium and by temperature. The highest concentration of essential oils was obtained when regenerated flowers were...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231941
更新日期:1995-06-01 00:00:00
abstract::Cultured cells of tobacco (Nicotiana tabacum L. cv Petit Havana) were used to investigate signals regulating the expression of the "model" nuclear gene encoding the alternative oxidase (AOX) (AOX1), the terminal oxidase of the mitochondrial alternative respiratory pathway. Several conditions shown to induce AOX1 mRNA ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0848-1
更新日期:2004-12-01 00:00:00
abstract:: Synthesis of five different Sudan-β-D-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroacetimidoyl-α-D-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000219
更新日期:2000-10-01 00:00:00
abstract:: Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-old nodular green ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050763
更新日期:2000-04-01 00:00:00
abstract::Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233096
更新日期:1992-10-01 00:00:00
abstract::Pigeon pea [Cajanus cajan (L.) Millsp.] (Family: Fabaceae) is an important legume crop cultivated across 50 countries in Asia, Africa, and the Americas; and ranks fifth in area among pulses after soybean, common bean, peanut, and chickpea. It is consumed as a major source of protein (21%) to the human population in ma...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-010-0899-4
更新日期:2010-10-01 00:00:00
abstract::Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232295
更新日期:1990-09-01 00:00:00
abstract::The expressed sequence tags (ESTs) referenced in this report are the first transcriptomes in a leaf from a half-shade ginseng plant. A cDNA library was constructed from samples of the leaves of 4-year-old Panax ginseng plants, which were cultured in a field. The 2,896 P. ginseng cDNA clones represent 1,576 unique sequ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0095-0
更新日期:2006-06-01 00:00:00
abstract::Heat shock transcription factors (Hsfs) are the terminal components of the signal transduction chain mediating the activation of genes responsive to both heat stress and a large number of chemical stressors. This paper aims to clone Hsf from lily and characterize its function by analyses of mRNA expression, transactiv...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0873-1
更新日期:2010-08-01 00:00:00
abstract:KEY MESSAGE:We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestans infection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different sig...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2144-x
更新日期:2017-07-01 00:00:00
abstract:UNLABELLED:The quality and productivity of chrysanthemum are severely compromised by various abiotic stresses. Here, we describe the isolation of CdICE1 from Chrysanthemum dichrum using RACE PCR, which shared identical nucleotide of ICE1 ORF from Chrysanthemum grandiflorum variety 'Jinba'. CdICE1 contains a conserved b...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1288-y
更新日期:2012-09-01 00:00:00
abstract::Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00778536
更新日期:1989-04-01 00:00:00
abstract::Through the use of Capsicum chromoplast membranes, we report for the first time the direct methylation of γ-tocopherol into α-tocopherol in the presence of S-adenosylmethionine. Furthermore the S-adenosylmethionine: γ-tocopherol methyltransferase activity has been solubilized. On a protein basis, the activity recovere...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00285499
更新日期:1985-02-01 00:00:00
abstract::Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were fou...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269896
更新日期:1985-12-01 00:00:00
abstract::The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269229
更新日期:1983-02-01 00:00:00
abstract::Plants were regenerated from leafy spurge (Euphorbia esula L.) cell suspensions obtained from stem callus. A North Dakota accession was highly regenerable, but two accessions from Oregon and Austria formed only a few plantlets. Organogenesis occurred in media without growth regulators, under fluorescent lights (30 to ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272536
更新日期:1988-06-01 00:00:00
abstract::The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00716844
更新日期:1989-02-01 00:00:00
abstract::A reliable protocol for the transformation of cultivated sunflower (Helianthus annuus L.) has been established, based on microprojectile bombardment of half shoot apices in combination with Agrobacterium tumefaciens coculture. Transgenic shoots have been obtained from 5 inbred lines, although transformation efficienci...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233766
更新日期:1994-12-01 00:00:00
abstract:KEY MESSAGE:Arabidopsis GI negatively regulates chloroplast biogenesis and resistance to the herbicide butafenacil by enhanced activity and transcriptional levels of antioxidant enzymes Chloroplast biogenesis is blocked by retrograde signaling triggered by diverse internal and external cues, including sugar, reactive o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02409-x
更新日期:2019-07-01 00:00:00
abstract::The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-(13)C] proline was followed by (13)C NMR and ninhydrin analyses of suspensions cultured...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269924
更新日期:1988-08-01 00:00:00
abstract::High yields of viable protoplasts were obtained by enzymatic treatment from cotyledonary leaves of various greenhouse grown Phaseolus vulgaris L. cultivars. The protoplasts divided and formed cell clusters in a liquid medium. Early transfer before 10 days in the same medium was necessary for the development of cell co...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269250
更新日期:1986-04-01 00:00:00
abstract::Albinism remains a major problem in cereal improvement programs that rely on doubled haploid (DH) technology, and the factors controlling the phenomenon are not well understood. Here we report on the positive influence of copper on the production of DH plants obtained through microspore embryogenesis (ME) in recalcitr...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0733-z
更新日期:2009-09-01 00:00:00