A simple system for pea transformation.

Abstract:

:The lateral cotyledonary meristems of germinatingPisum sativum cv. Puget seeds were used to develop a reproducibleAgrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal, self-fertile plants containing functional transgenes inherited in a Mendelian fashion.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Bean SJ,Gooding PS,Mullincaux PM,Davies DR

doi

10.1007/BF01142315

subject

Has Abstract

pub_date

1997-05-01 00:00:00

pages

513-519

issue

8

eissn

0721-7714

issn

1432-203X

pii

10.1007/BF01142315

journal_volume

16

pub_type

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