Isolation of protoplasts and regeneration of callus from suspension cultures of cultivated beets.

Abstract:

:Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were found to be quite simple: protoplasts could be cultured in MS, B5 or PGo based media with 0.4 M glucose with the optimum result being produced on KM8p medium. Plating efficiency (P.E) was genotype-dependent with the sugar beet giving better P.E. than the fodder or garden beets used, and higher values being achieved with the use of desalted Driselase for isolation followed by culture on KMBp medium.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Bhat SR,Ford-Lloyd BV,Callow JA

doi

10.1007/BF00269896

subject

Has Abstract

pub_date

1985-12-01 00:00:00

pages

348-50

issue

6

eissn

0721-7714

issn

1432-203X

journal_volume

4

pub_type

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