Abstract:
:Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of the tested media incorporating auxins, while shoots were observed on media containing exclusively 6-benzylaminopurine. Fertile plants were recovered from all explant types under a wide range of conditions, albeit with different efficiencies. The induction mechanism of the observed shoots is discussed.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Laparra H,Stoeva P,Ivanov P,Hahne Gdoi
10.1007/s002990050303subject
Has Abstractpub_date
1997-07-01 00:00:00pages
692-695issue
10eissn
0721-7714issn
1432-203Xpii
10.1007/s002990050303journal_volume
16pub_type
杂志文章abstract::A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269202
更新日期:1985-04-01 00:00:00
abstract:KEY MESSAGE:Reciprocal effects were found for tomato fruit quality and DNA methylation. The epigenetic identity of reciprocal hybrids indicates that DNA methylation might be one of the mechanisms involved in POEs. Crosses between different genotypes and even between different species are commonly used in plant breeding...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02624-x
更新日期:2021-01-01 00:00:00
abstract:KEY MESSAGE:Autotetraploid Arabidopsis line esd and 4COL exhibit enhanced tolerance to Cu stress by enhancing activation of antioxidative defenses, altering expression of genes related to Cu transport, chelation, and ABA-responsive. Autopolyploidy is ubiquitous among angiosperms and often results in better adaptation t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2176-2
更新日期:2017-10-01 00:00:00
abstract::The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-(13)C] proline was followed by (13)C NMR and ninhydrin analyses of suspensions cultured...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269924
更新日期:1988-08-01 00:00:00
abstract::Shoot cultures of Allium sativum L. were established from stem-tip and scape-tip. Various combinations of media, growth regulators were compared. The best establishment and proliferation of stem-tip and scape-tip explants were obtained on MS medium containing 0.5 mg l(-1) NAA and 0.5 mg l(-1) KT. The roots were produc...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233301
更新日期:1994-11-01 00:00:00
abstract::The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segmen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0604-y
更新日期:2003-04-01 00:00:00
abstract::The voltage-dependent anion channels (VDACs), mitochondrial outer membrane components, are present in organisms from fungi to animals and plants. They are thought to function in the regulation of metabolite transport between mitochondria and the cytoplasm. Sufficient knowledge on plant VDACs has been accumulated, so t...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-009-0741-z
更新日期:2009-09-01 00:00:00
abstract::Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes. The transformed cultures of ginseng could be subcultured as an axenic root culture in the absence of phytohormones, and grew with extensive lateral branches more rapidly t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272780
更新日期:1987-12-01 00:00:00
abstract::Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193724
更新日期:1995-12-01 00:00:00
abstract::The DNA-binding domain of the yeast transcriptional activator GAL4 was expressed in transgenic tobacco plants in order to attempt to obtain specific repression of reporter genes. Expression of the GUS and the NPTII gene was essentially the same regardless of the presence of a gene encoding a functional GAL4 DNA-bindin...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232920
更新日期:1995-10-01 00:00:00
abstract::Callus cultures of Vitis labruscana cv. Concord were initiated from both immature fruit and petiole tissue. Analysis revealed that the acid hydrolyzable precursors to β-damascenone, a compound important to the flavor of most grape species, were present in both callus types. Light and increased sucrose concentration we...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231971
更新日期:1994-05-01 00:00:00
abstract::An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1583-x
更新日期:2014-06-01 00:00:00
abstract::Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239891
更新日期:1994-01-01 00:00:00
abstract:KEY MESSAGE:High and low resveratrol (Res) contents in two cultivars are correlated with the expression abundance of Myb14 , which could directly activate transcriptional expression of stilbene synthase gene ( STS ). Resveratrol (3,5,4'-trihydroxystilbene) is one of the natural polyphenols produced by secondary metabol...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1642-3
更新日期:2014-10-01 00:00:00
abstract::A cDNA clone encoding a putative RTE1-like protein (Rh-RTH1) was obtained from total RNA isolated from senescing rose (Rosa hybrida cv. Tineke) petals using RT-PCR and RACE techniques. The cDNA (1,061 bp) contained an open reading frame of 684 bp corresponding to 227 amino acids. The amino acid sequence had 60.0, 49.6...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0875-z
更新日期:2010-08-01 00:00:00
abstract::Cell structures were compared between alkaloid-producing and non-producing cell cultures of Coptis japonica and Thalictrum minus by electron microscopic observation. In alkaloid-producing cells of C. japonica, prior to the onset of alkaloid synthesis, the vacuoles showed a greater volume than in non-producing cells. T...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269721
更新日期:1986-02-01 00:00:00
abstract::Protoplasts derived from oat (Avena sativa L.) suspension culture cells (7 days after subculturing) were electroporated with plasmid DNA containing the Escherichia coli uidA gene encoding the ß-glucuronidase reporter enzyme. Consistently high enzyme activity was observed with electroporation conditions of 500 μF and 1...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234710
更新日期:1993-05-01 00:00:00
abstract::The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed. The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0097-y
更新日期:2006-04-01 00:00:00
abstract::While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1073-3
更新日期:2011-09-01 00:00:00
abstract::High yields of viable protoplasts were obtained by enzymatic treatment from cotyledonary leaves of various greenhouse grown Phaseolus vulgaris L. cultivars. The protoplasts divided and formed cell clusters in a liquid medium. Early transfer before 10 days in the same medium was necessary for the development of cell co...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269250
更新日期:1986-04-01 00:00:00
abstract::Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium pH on strain virulence. Tumours were incited on all dico...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00235354
更新日期:1991-04-01 00:00:00
abstract::Euphorbia dulcis endosperm is the site of controlled long lasting endocellular lysis involving segregation and autophagy of portions of the cytoplasm within the endoplasmic reticulum membrane. The lysis products may constitute a food source for the benefit of the early developing embryo. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231924
更新日期:1996-05-01 00:00:00
abstract::In an attempt to improve growth and secondary metabolite production, and to understand the possible mechanism involved in relation to the changes in physiology and activities of antioxidant enzymes, we cultured Morinda citrifolia adventitious roots in different strength (0.25, 0.50, 0.75, 1.0, 1.5 and 2.0) of Murashig...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0854-4
更新日期:2010-07-01 00:00:00
abstract::Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269296
更新日期:1984-12-01 00:00:00
abstract::Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0672-z
更新日期:2003-09-01 00:00:00
abstract::The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01890855
更新日期:1996-12-01 00:00:00
abstract::The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenou...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0046-9
更新日期:2006-03-01 00:00:00
abstract::The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0664-0
更新日期:2009-04-01 00:00:00
abstract::The expressed sequence tags (ESTs) referenced in this report are the first transcriptomes in a leaf from a half-shade ginseng plant. A cDNA library was constructed from samples of the leaves of 4-year-old Panax ginseng plants, which were cultured in a field. The 2,896 P. ginseng cDNA clones represent 1,576 unique sequ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0095-0
更新日期:2006-06-01 00:00:00
abstract::To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0766-3
更新日期:2009-11-01 00:00:00