Comparative analysis of cell cycle events in zygotic and somatic embryos of Cyclamen persicum indicates strong resemblance of somatic embryos to recalcitrant seeds.

abstract：KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...

journal_title：Plant cell reports

authors： Coronel CJ,González AI,Ruiz ML,Polanco C

更新日期：2018-01-01 00:00:00

abstract：:Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of a...

journal_title：Plant cell reports

authors： Pietrosiuk A,Sykłowska-Baranek K,Wiedenfeld H,Wolinowska R,Furmanowa M,Jaroszyk E

更新日期：2006-10-01 00:00:00

abstract：KEY MESSAGE:We developed a method for detection of mutants in a large number of plants, and found this method to be applicable to detection of a mutant allele at a concentration of 1/1000. Many techniques for SNP analysis have been developed, but most of these techniques are not so sensitive to be used for detection of...

journal_title：Plant cell reports

authors： Kohata R,Koitabashi K,Kitashiba H,Nishio T

更新日期：2018-06-01 00:00:00

abstract：:This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...

journal_title：Plant cell reports

authors： Caillot S,Rosiau E,Laplace C,Thomasset B

更新日期：2009-03-01 00:00:00

abstract：:LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...

journal_title：Plant cell reports

authors： Touno K,Harada K,Yoshimatsu K,Yazaki K,Shimomura K

更新日期：2000-11-01 00:00:00

abstract：KEY MESSAGE:The Arabidopsis AP2/ERF family transcription factor AtERF15 is nuclear-localized and positively regulates ABA and stress responses. Abscisic acid (ABA) is a major plant hormone that controls the expression of hundreds genes involved in various aspects of plant growth and development, such as seed developmen...

journal_title：Plant cell reports

authors： Lee SB,Lee SJ,Kim SY

更新日期：2015-01-01 00:00:00

abstract：:A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protop...

journal_title：Plant cell reports

authors： Edwards ES,Roux SJ

更新日期：1998-06-01 00:00:00

abstract：:Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived...

journal_title：Plant cell reports

authors： Darsan Singh JK,Mat Jalaluddin NS,Sanan-Mishra N,Harikrishna JA

更新日期：2019-12-01 00:00:00

abstract：KEY MESSAGE:The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Despite our knowledge of their advantages, plant-based vaccines remain unavailable ...

journal_title：Plant cell reports

authors： Kashima K,Yuki Y,Mejima M,Kurokawa S,Suzuki Y,Minakawa S,Takeyama N,Fukuyama Y,Azegami T,Tanimoto T,Kuroda M,Tamura M,Gomi Y,Kiyono H

更新日期：2016-03-01 00:00:00

abstract：:Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...

journal_title：Plant cell reports

authors： Aviv D,Chen R,Galun E

更新日期：1986-06-01 00:00:00

abstract：:Protoplasts isolated enzymatically from seedling roots, hypocotyls and cotyledons of Lotus corniculatus L. produced callus which underwent prolific shoot regeneration. The rapidity and ease of recovering plants from protoplast-derived tissues makes this forage legume an attractive experimental system for genetic manip...

journal_title：Plant cell reports

authors： Ahuja PS,Hadiuzzaman S,Davey MR,Cocking EC

更新日期：1983-04-01 00:00:00

abstract：:A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yi...

journal_title：Plant cell reports

authors： Chang YF,Wang WC,Warfield CY,Nguyen HT,Wong JR

更新日期：1991-03-01 00:00:00

abstract：:Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos dev...

journal_title：Plant cell reports

authors： Xu N,Bewley JD

更新日期：1992-06-01 00:00:00

abstract：:Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, sug...

journal_title：Plant cell reports

authors： Rodríguez-Concepción M,Gómez MD,Beltrán JP

更新日期：1996-04-01 00:00:00

abstract：:Shoot buds of ginger were successfully encapsulated in 4% sodium alginate gel. Encapsulated buds were germinated in vitro to form roots and shoots. In vitro germination (emergence of sprouts) of encapsulated buds ranged from 16.7% to 81.8% on different media after 5 weeks of incubation. Normal plantlets with an averag...

journal_title：Plant cell reports

authors： Sharma TR,Singh BM,Chauhan RS

更新日期：1994-02-01 00:00:00

abstract：:Callus cultures have been established from root explants of aseptically-grown Podophyllum hexandrum seedlings. A fully defined medium based on Gamborg's B5 salts supplemented with 2/4-dichlorophenoxyacetic acid, gibberellic acid and 6-benzylaminopurine was effective for both initiation and sustained growth of callus t...

journal_title：Plant cell reports

authors： Heyenga AG,Lucas JA,Dewick PM

更新日期：1990-11-01 00:00:00

abstract：:This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that wer...

journal_title：Plant cell reports

authors： Igasaki T,Sato T,Akashi N,Mohri T,Maruyama E,Kinoshita I,Walter C,Shinohara K

更新日期：2003-11-01 00:00:00

abstract：:Donor plants of Hordeum vulgare L. cv. Igri were grown in a conditioned environment to minimise fluctuations in the composition of the microspore population. After isolation different types of microspores were identified within each population, amongst others an embryogenic subpopulation. It was shown that the optimum...

journal_title：Plant cell reports

authors： Hoekstra S,van Zijderveld MH,Heidekamp F,van der Mark F

更新日期：1993-10-01 00:00:00

abstract：KEY MESSAGE:The cytokinin pathway promotes the initiation of bulbil formation, and iPA may an important type of cytokinin during bulbil formation in Lilium lancifolium. Bulbils are important vegetative reproductive organs in triploid Lilium lancifolium. We previously showed that cytokinins are involved in bulbil format...

journal_title：Plant cell reports

authors： He G,Yang P,Tang Y,Cao Y,Qi X,Xu L,Ming J

更新日期：2020-07-01 00:00:00

abstract：:A differential expression study on the Saccharum spp.-Sporisorium scitamineum pathogenic interaction was carried out in the susceptible Ja60-5 and the resistant M31/45 genotypes. Using cDNA-AFLP analysis, a total of 64 transcript-derived fragments (TDFs) was found to be differentially expressed, with the majority (67....

journal_title：Plant cell reports

authors： Lao M,Arencibia AD,Carmona ER,Acevedo R,Rodríguez E,León O,Santana I

更新日期：2008-06-01 00:00:00

abstract：:The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35...

journal_title：Plant cell reports

authors： Shirasawa-Seo N,Sano Y,Nakamura S,Murakami T,Gotoh Y,Naito Y,Hsia CN,Seo S,Mitsuhara I,Kosugi S,Ohashi Y

更新日期：2005-06-01 00:00:00

abstract：:In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...

journal_title：Plant cell reports

authors： Liu S,Kriz A,Duncan D,Widholm J

更新日期：1998-05-01 00:00:00

abstract：KEY MESSAGE:Sustainability and safety aspects of plant cell cultures as food are presented. Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown. Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agr...

journal_title：Plant cell reports

authors： Häkkinen ST,Nygren H,Nohynek L,Puupponen-Pimiä R,Heiniö RL,Maiorova N,Rischer H,Ritala A

更新日期：2020-12-01 00:00:00

abstract：:Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata ...

journal_title：Plant cell reports

authors： Kwa SH,Wee YC,Loh CS

更新日期：1991-10-01 00:00:00

abstract：:Glyoxalase-I activity in growingDatura callus showed 184% increase with the age of the culture. Spermidine increased the enzyme activity together with DNA and protein synthesis. With the addition of mitotic inhibitors, vinblastine and methylglyoxal in the growth medium, the enzyme activity was inhibited by 92 percent ...

journal_title：Plant cell reports

authors： Ramaswamy O,Pal S,Guha-Mukherjee S,Sopory SK

更新日期：1984-06-01 00:00:00

abstract：KEY MESSAGE:TaUBA functions as a negative regulator of salt and drought stress response in transgenic Arabidopsis, either the UBA domain or the zinc finger domain is crucial for TaUBA's function. TaUBA (DQ211935), which is a UBA domain-containing protein in wheat, was cloned and functionally characterized. Southern blo...

journal_title：Plant cell reports

authors： Li X,Zhang SS,Ma JX,Guo GY,Zhang XY,Liu X,Bi CL

更新日期：2015-05-01 00:00:00

abstract：:To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...

journal_title：Plant cell reports

authors： Patil G,Deokar A,Jain PK,Thengane RJ,Srinivasan R

更新日期：2009-11-01 00:00:00

abstract：:Plants have their own mechanisms for overcoming various stresses. In cold regions, plants are subject to stress and must enter an inherent dormancy, through several complex mechanisms, if they are to continue to exist. In winter, regulation of tonoplast and plasma membrane aquaporin genes differed in the bud cushions ...

journal_title：Plant cell reports

authors： Yooyongwech S,Sugaya S,Sekozawa Y,Gemma H

更新日期：2009-11-01 00:00:00

abstract：:Head-space volatiles above embryogenicPicea sitchensis (Bong.) Carr. (Sitka spruce) tissues cultured in glass Petri dishes sealed with Parafilm M or cling-film, were captured on Tenax adsorption traps and analysed by gas chromatography / mass spectrometry. Each sealing system released a single major compound into the ...

journal_title：Plant cell reports

authors： Selby C,McRoberts WC,Hamilton JT,Harvey BM

更新日期：1996-12-01 00:00:00

abstract：:Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...

journal_title：Plant cell reports

authors： Hippolyte I,Marin B,Baccou JC,Jonard R

更新日期：1992-04-01 00:00:00