Production of aposporous gametophytes and calli from Pteris vittata L. pinnae strips cultured in vitro.

Abstract:

:Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata cultured in vitro at lower concentrations of all the mentioned components. Aposporous gametophytes and vegetative calli were produced at higher concentrations. The calli regenerated sporophytes when cultured on MS medium without growth regulators. The gametophytes grew vegetatively on MS medium but produced sporophytes when transferred into 0.1 strength MS medium. This is the first report of simultaneous production of calli and gametophytes from fern explants.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Kwa SH,Wee YC,Loh CS

doi

10.1007/BF00232608

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

392-3

issue

8

eissn

0721-7714

issn

1432-203X

journal_volume

10

pub_type

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