Transcriptome-based gene expression profiling identifies differentially expressed genes critical for salt stress response in radish (Raphanus sativus L.).


KEY MESSAGE:Transcriptome-based gene expression analysis identifies many critical salt-responsive genes in radish and facilitates further dissecting the molecular mechanism underlying salt stress response. Salt stress severely impacts plant growth and development. Radish, a moderately salt-sensitive vegetable crop, has been studied for decades towards the physiological and biochemical performances under salt stress. However, no systematic study on isolation and identification of genes involved in salt stress response has been performed in radish, and the molecular mechanism governing this process is still indistinct. Here, the RNA-Seq technique was applied to analyze the transcriptomic changes on radish roots treated with salt (200 mM NaCl) for 48 h in comparison with those cultured in normal condition. Totally 8709 differentially expressed genes (DEGs) including 3931 up- and 4778 down-regulated genes were identified. Functional annotation analysis indicated that many genes could be involved in several aspects of salt stress response including stress sensing and signal transduction, osmoregulation, ion homeostasis and ROS scavenging. The association analysis of salt-responsive genes and miRNAs exhibited that 36 miRNA-mRNA pairs had negative correlationship in expression trends. Reverse-transcription quantitative PCR (RT-qPCR) analysis revealed that the expression profiles of DEGs were in line with results from the RNA-Seq analysis. Furthermore, the putative model of DEGs and miRNA-mediated gene regulation was proposed to elucidate how radish sensed and responded to salt stress. This study represents the first comprehensive transcriptome-based gene expression profiling under salt stress in radish. The outcomes of this study could facilitate further dissecting the molecular mechanism underlying salt stress response and provide a valuable platform for further genetic improvement of salt tolerance in radish breeding programs.


Plant Cell Rep


Plant cell reports


Sun X,Xu L,Wang Y,Luo X,Zhu X,Kinuthia KB,Nie S,Feng H,Li C,Liu L




Has Abstract


2016-02-01 00:00:00














  • Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.).

    abstract::The efficacy of benzyladenine (BA) to induce multiple shoots from seed explants of chickpea (Cicer arietinum L.) was assessed. Shoot differentiation was influenced by the type of seed explant, genotype and concentration of BA. Orientation of the explant also strongly influenced the shoot regeneration response. The opt...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Polisetty R,Paul V,Deveshwar JJ,Khetarpal S,Suresh K,Chandra R

    更新日期:1997-05-01 00:00:00

  • Improved nitrogen use efficiency in transgenic sugarcane: phenotypic assessment in a pot trial under low nitrogen conditions.

    abstract:KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Snyman SJ,Hajari E,Watt MP,Lu Y,Kridl JC

    更新日期:2015-05-01 00:00:00

  • Ploidy variation among herbicide-resistant bermudagrass plants of cv. TifEagle transformed with the bar gene.

    abstract::A protocol was developed for biolistic transformation of hybrid bermudagrass cv. TifEagle using the bar gene. TifEagle is an ultradwarf used exclusively on golf greens. Herbicide resistance should serve as a useful management tool, especially if methyl-bromide is unavailable for fumigation prior to plant establishment...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Goldman JJ,Hanna WW,Fleming GH,Ozias-Akins P

    更新日期:2004-03-01 00:00:00

  • Gynogenic plant regeneration from unpollinated flower explants of Eragrostis tef (Zuccagni) Trotter.

    abstract::Tef [Eragrostis tef (Zucc.) Trotter] is the most important cereal in Ethiopia. In its wild relative E. mexicana, regeneration of six green plants resulted from culture of 121 non-pollinated immature pistils. In the allotetraploid crop species tef, however, only callus and root formation was obtained by this method. By...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gugsa L,Sarial AK,Lörz H,Kumlehn J

    更新日期:2006-12-01 00:00:00

  • Somatic embryogenesis and organogenesis in callus cultures of a tree legume - Albizia richardiana King.

    abstract::Hypocotyl explants of 1 and 10 mm lengths were excised from 12-day-old in vitro-grown seedlings of Albizia richardiana. The larger pieces, after 40 days of culture, developed shoots along with green calli on B5 + BAP (10(-7)-10(-5)M), while the smaller segments produced only green calli on B5+BAP (10(-7)-10(-4)M) medi...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Tomar UK,Gupta SC

    更新日期:1988-01-01 00:00:00

  • Auxin and gibberellin responsive Arabidopsis SMALL AUXIN UP RNA36 regulates hypocotyl elongation in the light.

    abstract:KEY MESSAGE:The Arabidopsis SAUR36, renamed RAG1, integrates auxin and gibberellin signals to regulate apical hook maintenance in etiolated seedlings, hypocotyl elongation in the light and fertility. Phytohormone signalling intermediates integrate responses to developmental cues and the variety of environmental inputs ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Stamm P,Kumar PP

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  • Molecular cloning and characterization of the light-regulation and circadian-rhythm of the VDE gene promoter from Zingiber officinale.

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    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Zhao W,Wang S,Li X,Huang H,Sui X,Zhang Z

    更新日期:2012-08-01 00:00:00

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    journal_title:Plant cell reports

    pub_type: 杂志文章


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    更新日期:1985-05-01 00:00:00

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    journal_title:Plant cell reports

    pub_type: 杂志文章


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    journal_title:Plant cell reports

    pub_type: 杂志文章


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    更新日期:2016-11-01 00:00:00

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    journal_title:Plant cell reports

    pub_type: 杂志文章


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    更新日期:1995-10-01 00:00:00

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    abstract::The purpose of this study was to develop a new micropropagation system for Tylophora indica, an important medicinal plant in India, using root explants as starting material. Root explants cultured on MS medium supplemented with 6-benzyladenine (BA) or 2-isopentyladenine (2iP) produced organogenic nodular meristemoids ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Chaudhuri KN,Ghosh B,Jha S

    更新日期:2004-05-01 00:00:00

  • Efficient plant regeneration from hypocotyl protoplasts of broccoli (Brassica oleracea L. ssp. italica Plenck).

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    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Kao HM,Keller WA,Gleddie S,Brown GG

    更新日期:1990-10-01 00:00:00

  • Improved production of doubled haploids by colchicine application to wheat (Triticum aestivum L.) anther culture.

    abstract::The aim of this study was to optimize the in vitro chromosome-doubling procedure in wheat anther culture. Colchicine, at concentrations of 100-5000 mg/l, was added to the induction medium for 1-5 days. Beneficial effects were obtained with concentrations of 100 and 1000 mg/l colchicine. With time, significant reductio...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Redha A,Attia T,Büter B,Saisingtong S,Stamp P,Schmid JE

    更新日期:1998-09-01 00:00:00

  • Genotype- and promoter-induced variability in transient β-glucuronidase expression in pea protoplasts.

    abstract::Leaf mesophyll protoplasts isolated from pea (Pisum sativum L.) genotypes Century and PI244253 showed transient expression of β-glucuronidase (GUS) when electroporated with plasmid DNA containing various promoter-leader sequence constructs driving the GUS gene. The optimum conditions for transient expression were: usi...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hobbs SL,Jackson JA,Baliski DS,Delong CM,Mahon JD

    更新日期:1990-06-01 00:00:00

  • Low night temperature acclimation of Phalaenopsis.

    abstract::The capability of Phalaenopsis to acclimate its photosynthetic capacity and metabolic activity to cool night temperature conditions is crucial for improving orchid production in terms of efficient greenhouse heating. The extent to which Phalaenopsis possesses acclimation potential and the mechanistic background of the...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Pollet B,Vanhaecke L,Dambre P,Lootens P,Steppe K

    更新日期:2011-06-01 00:00:00

  • Plant regeneration from different explants in Helianthus smithii Heiser.

    abstract::Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of t...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Laparra H,Stoeva P,Ivanov P,Hahne G

    更新日期:1997-07-01 00:00:00

  • Competitive interaction between two functional S-haplotypes confer self-compatibility on tetraploid Chinese cherry (Prunus pseudocerasus Lindl. CV. Nanjing Chuisi).

    abstract::Self-incompatibility (SI) has been studied extensively at the molecular level in Solanaceae, Rosaceae and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility (GSI). In the present study, four PpsS-haplotypes (Prunus pseudocerasus S-haplotypes) comprising at least two genes, i.e., PpsS-RNase (P. ps...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Huang SX,Wu HQ,Li YR,Wu J,Zhang SJ,Heng W,Zhang SL

    更新日期:2008-06-01 00:00:00

  • Successful recovery of transgenic cowpea (Vigna unguiculata) using the 6-phosphomannose isomerase gene as the selectable marker.

    abstract::A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-d...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Bakshi S,Saha B,Roy NK,Mishra S,Panda SK,Sahoo L

    更新日期:2012-06-01 00:00:00

  • Sequential rooting media and rooting capacity of Sequoiadendron giganteum in vitro. Peroxidase activity as a marker.

    abstract::The rooting capacities of tips of seedling, juvenile and mature shoots of Sequoiadendron giganteum were compared on different rooting media (inductive and expressive media) after passage on an elongating medium. None of the cuttings rooted when continuously kept on medium containing the auxin NAA and vitamin D2. Perox...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Berthon JY,Boyer N,Gaspar T

    更新日期:1987-10-01 00:00:00

  • RNAseq analysis of cassava reveals similar plant responses upon infection with pathogenic and non-pathogenic strains of Xanthomonas axonopodis pv. manihotis.

    abstract:KEY MESSAGE:An RNAseq-based analysis of the cassava plants inoculated with Xam allowed the identification of transcriptional upregulation of genes involved in jasmonate metabolism, phenylpropanoid biosynthesis and putative targets for a TALE. Cassava bacterial blight, a disease caused by the gram-negative bacterium Xan...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Muñoz-Bodnar A,Perez-Quintero AL,Gomez-Cano F,Gil J,Michelmore R,Bernal A,Szurek B,Lopez C

    更新日期:2014-11-01 00:00:00

  • Establishment of a transgenic hairy root system in wild and domesticated watermelon (Citrullus lanatus) for studying root vigor under drought.

    abstract::Root vigor is an important trait for the growth of terrestrial plants, especially in water-deficit environments. Although deserts plants are known for their highly developed root architecture, the molecular mechanism responsible for this trait has not been determined. Here we established an efficient protocol for the ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Kajikawa M,Morikawa K,Abe Y,Yokota A,Akashi K

    更新日期:2010-07-01 00:00:00

  • Putative sites of cytokinin action during their enhancing effect on indole alkaloid accumulation in periwinkle cell suspensions.

    abstract::The effects of cytokinins on the different branches of the indole alkaloid pathway were investigated in Catharanthus roseus cell cultures. Addition of zeatin to a 2,4-dichlorophenoxyacetic acid-containing medium decreased tryptamine levels and increased the bioconversion of secologanin to ajmalicine. Zeatin also enhan...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Decendit A,Petit G,Andreu F,Doireau P,Mérillon JM,Rideau M

    更新日期:1993-10-01 00:00:00

  • Enzymatic synthesis of 6'-deoxychalcone in cultured Glycyrrhiza echinata cells.

    abstract::5-Deoxy-(iso)flavonoids are biosynthesized from 6'-deoxychalcone (isoliquiritigenin). The coaction of a reductase with chalcone synthase (CHS) has been established in soybean cells to be responsible for the synthesis of 6'-deoxychalcone. Western blot analysis of crude extracts from cultured cells of Glycyrrhiza echina...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Haranô K,Okada N,Furuno T,Takahashi T,Ayabe S,Welle R

    更新日期:1993-01-01 00:00:00

  • Establishment of a novel tissue culture method, stem-disc culture, and its practical application to micropropagation of garlic (Allium sativum L.).

    abstract::A restricted part of the undeveloped stem of the garlic clove, called the "stem disc", which is just under the basement of the immature foliage leaves, proved to be a very potent explant for the micropropagation of garlic. Twenty to thirty tissue-cultured shoots consistently were differentiated from a single clove dur...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ayabe M,Sumi S

    更新日期:1998-07-01 00:00:00

  • Effects of agrobacterial oncogenes in kidney vetch (Anthyllis vulneraria L.).

    abstract::Kidney vetch seedlings were induced to form hairy roots by inoculating their mesocotyls with the wild-type strain 15834 of Agrobacterium rhizogenes or with the A. tumefaciens strain C58C1 containing a binary vector system (the pRiA4b as a helper and the vector pCB1346 bearing a pTiC58-derived isopentenyl transferase g...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Stiller J,Sinec VN,Svoboda S,Němcová B,Macháčková I

    更新日期:1992-07-01 00:00:00

  • A novel strategy for the induction and maintenance of shoot regeneration from callus derived from established shoots of apple [Malus×Domestica Borkh.] cv. Golden Delicious.

    abstract::A novel strategy for the production and maintenance of morphogenic callus for 1 year from mature leaf explants of apple has been developed using micropropagated primary leaves of cv. Golden Delicious. The technique required second generation adventitious buds produced from cultured primary leaves also produced from es...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Rugini E,Muganu M

    更新日期:1998-04-01 00:00:00

  • Defense gene expression in elicitor-treated cell suspension cultures of french bean cv. Imuna.

    abstract::Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). How...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ellis JS,Jennings AC,Edwards LA,Mavandad M,Lamb CJ,Dixon RA

    更新日期:1989-12-01 00:00:00

  • Myb14, a direct activator of STS, is associated with resveratrol content variation in berry skin in two grape cultivars.

    abstract:KEY MESSAGE:High and low resveratrol (Res) contents in two cultivars are correlated with the expression abundance of Myb14 , which could directly activate transcriptional expression of stilbene synthase gene ( STS ). Resveratrol (3,5,4'-trihydroxystilbene) is one of the natural polyphenols produced by secondary metabol...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Fang L,Hou Y,Wang L,Xin H,Wang N,Li S

    更新日期:2014-10-01 00:00:00

  • Level of tissue differentiation influences the activation of a heat-inducible flower-specific system for genetic containment in poplar (Populus tremula L.).

    abstract:KEY MESSAGE:Differentiation level but not transgene copy number influenced activation of a gene containment system in poplar. Heat treatments promoted CRE gene body methylation. The flower-specific transgene deletion was confirmed. Gene flow between genetic modified trees and their wild relatives is still motive of con...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hoenicka H,Lehnhardt D,Nunna S,Reinhardt R,Jeltsch A,Briones V,Fladung M

    更新日期:2016-02-01 00:00:00