Transcriptome-based gene expression profiling identifies differentially expressed genes critical for salt stress response in radish (Raphanus sativus L.).


KEY MESSAGE:Transcriptome-based gene expression analysis identifies many critical salt-responsive genes in radish and facilitates further dissecting the molecular mechanism underlying salt stress response. Salt stress severely impacts plant growth and development. Radish, a moderately salt-sensitive vegetable crop, has been studied for decades towards the physiological and biochemical performances under salt stress. However, no systematic study on isolation and identification of genes involved in salt stress response has been performed in radish, and the molecular mechanism governing this process is still indistinct. Here, the RNA-Seq technique was applied to analyze the transcriptomic changes on radish roots treated with salt (200 mM NaCl) for 48 h in comparison with those cultured in normal condition. Totally 8709 differentially expressed genes (DEGs) including 3931 up- and 4778 down-regulated genes were identified. Functional annotation analysis indicated that many genes could be involved in several aspects of salt stress response including stress sensing and signal transduction, osmoregulation, ion homeostasis and ROS scavenging. The association analysis of salt-responsive genes and miRNAs exhibited that 36 miRNA-mRNA pairs had negative correlationship in expression trends. Reverse-transcription quantitative PCR (RT-qPCR) analysis revealed that the expression profiles of DEGs were in line with results from the RNA-Seq analysis. Furthermore, the putative model of DEGs and miRNA-mediated gene regulation was proposed to elucidate how radish sensed and responded to salt stress. This study represents the first comprehensive transcriptome-based gene expression profiling under salt stress in radish. The outcomes of this study could facilitate further dissecting the molecular mechanism underlying salt stress response and provide a valuable platform for further genetic improvement of salt tolerance in radish breeding programs.


Plant Cell Rep


Plant cell reports


Sun X,Xu L,Wang Y,Luo X,Zhu X,Kinuthia KB,Nie S,Feng H,Li C,Liu L




Has Abstract


2016-02-01 00:00:00














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    authors: Shoyama Y,Zhu XX,Nakai R,Shiraishi S,Kohda H

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    更新日期:2017-11-01 00:00:00

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    authors: Beracochea VC,Almasia NI,Peluffo L,Nahirñak V,Hopp EH,Paniego N,Heinz RA,Vazquez-Rovere C,Lia VV

    更新日期:2015-10-01 00:00:00

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    abstract::A collection of 29 pathogenesis-related 10 (PR10) genes of Medicago sativa and Medicago truncatula showed that they were almost all obtained from cDNA libraries of tissues undergoing abiotic or biotic stresses. The predicted proteins could be divided into two subclasses, PR10.1 and PR10.2, but in silico predicted mode...

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    authors: Bahramnejad B,Goodwin PH,Zhang J,Atnaseo C,Erickson LR

    更新日期:2010-11-01 00:00:00

  • Site-specific recombination for genetic engineering in plants.

    abstract::Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements us...

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    authors: Lyznik LA,Gordon-Kamm WJ,Tao Y

    更新日期:2003-06-01 00:00:00

  • High-throughput assessment of transgene copy number in sugarcane using real-time quantitative PCR.

    abstract::Accurate and timely detection of transgene copy number in sugarcane is currently hampered by the requirement to use Southern blotting, needing relatively large amounts of genomic DNA and, therefore, the continued growth and maintenance of bulky plants in containment glasshouses. In addition, the sugarcane genome is bo...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Casu RE,Selivanova A,Perroux JM

    更新日期:2012-01-01 00:00:00

  • Physiological and transcriptomic responses of reproductive stage soybean to drought stress.

    abstract:KEY MESSAGE:The dynamic alterations of the physiological and molecular processes in reproductive stage soybean indicated the dramatic impact caused by drought. Drought is a major abiotic stress that limits soybean (Glycine max) production. Most prior studies were focused on either model species or crops that are at the...

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    pub_type: 杂志文章


    authors: Xu C,Xia C,Xia Z,Zhou X,Huang J,Huang Z,Liu Y,Jiang Y,Casteel S,Zhang C

    更新日期:2018-12-01 00:00:00

  • Stable transformation and regeneration of transgenic plants of Pinus radiata D. Don.

    abstract::A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...

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    pub_type: 杂志文章


    authors: Walter C,Grace LJ,Wagner A,White DWR,Walden AR,Donaldson SS,Hinton H,Gardner RC,Smith DR

    更新日期:1998-04-01 00:00:00

  • Differential expression analysis by cDNA-AFLP of Saccharum spp. after inoculation with the host pathogen Sporisorium scitamineum.

    abstract::A differential expression study on the Saccharum spp.-Sporisorium scitamineum pathogenic interaction was carried out in the susceptible Ja60-5 and the resistant M31/45 genotypes. Using cDNA-AFLP analysis, a total of 64 transcript-derived fragments (TDFs) was found to be differentially expressed, with the majority (67....

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    pub_type: 杂志文章


    authors: Lao M,Arencibia AD,Carmona ER,Acevedo R,Rodríguez E,León O,Santana I

    更新日期:2008-06-01 00:00:00

  • LchERF, a novel ethylene-responsive transcription factor from Lycium chinense, confers salt tolerance in transgenic tobacco.

    abstract:KEY MESSAGE:An ERF gene, LchERF , was cloned from L. chinense for the first time. Overexpression of LchERF conferred salt stress tolerance in transgenic tobacco lines during seed germination and vegetative growth. Ethylene-responsive transcription factors (ERFs) play important roles in tolerance to biotic and abiotic s...

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    pub_type: 杂志文章


    authors: Wu D,Ji J,Wang G,Guan C,Jin C

    更新日期:2014-12-01 00:00:00

  • Plant regeneration via somatic embryogenesis in creeping bentgrass (Agrostis palustris Huds.).

    abstract::We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Zhong H,Srinivasan C,Sticklen MB

    更新日期:1991-11-01 00:00:00

  • Cell reabsorption in the Euphorbia dulcis endosperm.

    abstract::Euphorbia dulcis endosperm is the site of controlled long lasting endocellular lysis involving segregation and autophagy of portions of the cytoplasm within the endoplasmic reticulum membrane. The lysis products may constitute a food source for the benefit of the early developing embryo. ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Muccifora S,Gori P

    更新日期:1996-05-01 00:00:00

  • Regeneration of plants from callus tissue of the pasture legume Centrosema brasilianum.

    abstract::Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and B...

    journal_title:Plant cell reports

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    authors: Angeloni PN,Rey HY,Mroginski LA

    更新日期:1992-09-01 00:00:00

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    abstract::Gastrodia elata Bl. is an achlorophyllous orchid plant feeding on the fungus Armillaria mellea. The plant lives underground during its life cycle except for florescence. Gastrodianins, members of the superfamily of monocot mannose-binding protein (MBP), have been identified from Gastrodia elata, yet their physiologica...

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    pub_type: 杂志文章


    authors: Wang HX,Yang T,Zeng Y,Hu Z

    更新日期:2007-03-01 00:00:00

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    abstract::Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide reduction through a plasma membrane redox system which may be associated with proton translocation. Evidence shows that endogenous pyridine nucleotides serve as hydrogen donors for the reaction. The proton translocating function of the redox system ...

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    authors: Marigo G,Belkoura M

    更新日期:1985-12-01 00:00:00

  • Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria x ananassa Duch.) using a range of explant types.

    abstract::The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf dis...

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    authors: Passey AJ,Barrett KJ,James DJ

    更新日期:2003-01-01 00:00:00

  • Can the world's favorite fruit, tomato, provide an effective biosynthetic chassis for high-value metabolites?

    abstract::Tomato has a relatively short growth cycle (fruit ready to pick within 65-85 days from planting) and a relatively high yield (the average for globe tomatoes is 3-9 kg fruit per plant rising to as much as 40 kg fruit per plant). Tomatoes also produce large amounts of important primary and secondary metabolites which ca...

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    authors: Li Y,Wang H,Zhang Y,Martin C

    更新日期:2018-10-01 00:00:00

  • Comparison of elicitor-based effects on metabolic responses of Taxus × media hairy roots in perfluorodecalin-supported two-phase culture system.

    abstract:KEY MESSAGE:Two lines of Taxus × media hairy roots harbouring or not the TXS transgene demonstrated diverse gene expression and taxane yield during cultivation in PFD-supported two liquid-phase culture system. Two lines of Taxus × media hairy roots were subjected to single or twice-repeated supplementation with methyl ...

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    authors: Sykłowska-Baranek K,Rymaszewski W,Gaweł M,Rokicki P,Pilarek M,Grech-Baran M,Hennig J,Pietrosiuk A

    更新日期:2019-01-01 00:00:00

  • Development of male gametophyte of Larix leptolepis Gord. with emphasis on diffuse stage of meiosis.

    abstract::A basic developmental framework of the Larix leptolepis Gord male gametophyte is presented in detail by squashing technique. The duration of the meiosis stage was more than 6 months, and included a long diffuse stage during winter. This long duration of the diffuse appearance of the diplotene stage makes L. leptolepis...

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    authors: Zhang SG,Yang WH,Qi YC,Li MX,Wang JH,Sun XM,Wang XS,Qi LW

    更新日期:2008-11-01 00:00:00

  • Cytological changes associated with alkaloid production in cultured cells of Coptis japonica and Thalictrum minus.

    abstract::Cell structures were compared between alkaloid-producing and non-producing cell cultures of Coptis japonica and Thalictrum minus by electron microscopic observation. In alkaloid-producing cells of C. japonica, prior to the onset of alkaloid synthesis, the vacuoles showed a greater volume than in non-producing cells. T...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Yamamoto H,Nakagawa K,Fukui H,Tabata M

    更新日期:1986-02-01 00:00:00

  • Effect of gibberellin biosynthesis inhibitors on shoot regeneration from hypocotyl explants of Albizzia julibrissin.

    abstract::Hypocotyl explants of Albizzia julibrissin were placed on Gamborg's B5 medium supplemented with various levels of paclobutrazol, uniconazole, prohexadione calcium, or GA3. Callus formation was evident within one week after placement of the explants on the culture media. Green nodule-like structures protruded from the ...

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    authors: Sankhla D,Davis TD,Sankhla N

    更新日期:1993-12-01 00:00:00

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    authors: Szopa J,Müller-Röber B

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  • Anthocyanin accumulation and changes in activities of phenylalanine ammonia-lyase and chalcone synthase in roselle (Hibiscus sabdariffa L.) callus cultures.

    abstract::Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or ligh...

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    pub_type: 杂志文章


    authors: Mizukami H,Tomita K,Ohashi H

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  • Asymmetric somatic hybridization between UV-irradiated Citrus unshiu and C. sinensis: regeneration and characterization of hybrid shoots.

    abstract::In the present paper, attempts were made to explore the possibility of employing ultraviolet (UV) irradiation in citrus asymmetric fusion for transfer of limited amount of favorable traits from a desirable cultivar to a target one. Exposure of Satsuma mandarin (Citrus unshiu Marc.) embryogenic protoplasts to UV at an ...

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    pub_type: 杂志文章


    authors: Xu XY,Hu ZY,Li JF,Liu JH,Deng XX

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  • Agrobacterium induced gall formation in lipoxygenase mutant isolines of soybeans.

    abstract::Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos m...

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    pub_type: 杂志文章


    authors: Bhatt D,Parrott WA,Collins GB,Hildebrand DF

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  • Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers.

    abstract:KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...

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    authors: Coronel CJ,González AI,Ruiz ML,Polanco C

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    pub_type: 杂志文章


    authors: Polóniová Z,Jopčík M,Matušíková I,Libantová J,Moravčíková J

    更新日期:2015-03-01 00:00:00

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    abstract::Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...

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    pub_type: 杂志文章


    authors: Toivonen L,Laakso S,Rosenqvist H

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  • Somatic embryogenesis and in vitro flowering of 3 species of bamboo.

    abstract::Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Rout GR,Das P

    更新日期:1994-09-01 00:00:00