Transcriptome-based gene expression profiling identifies differentially expressed genes critical for salt stress response in radish (Raphanus sativus L.).


KEY MESSAGE:Transcriptome-based gene expression analysis identifies many critical salt-responsive genes in radish and facilitates further dissecting the molecular mechanism underlying salt stress response. Salt stress severely impacts plant growth and development. Radish, a moderately salt-sensitive vegetable crop, has been studied for decades towards the physiological and biochemical performances under salt stress. However, no systematic study on isolation and identification of genes involved in salt stress response has been performed in radish, and the molecular mechanism governing this process is still indistinct. Here, the RNA-Seq technique was applied to analyze the transcriptomic changes on radish roots treated with salt (200 mM NaCl) for 48 h in comparison with those cultured in normal condition. Totally 8709 differentially expressed genes (DEGs) including 3931 up- and 4778 down-regulated genes were identified. Functional annotation analysis indicated that many genes could be involved in several aspects of salt stress response including stress sensing and signal transduction, osmoregulation, ion homeostasis and ROS scavenging. The association analysis of salt-responsive genes and miRNAs exhibited that 36 miRNA-mRNA pairs had negative correlationship in expression trends. Reverse-transcription quantitative PCR (RT-qPCR) analysis revealed that the expression profiles of DEGs were in line with results from the RNA-Seq analysis. Furthermore, the putative model of DEGs and miRNA-mediated gene regulation was proposed to elucidate how radish sensed and responded to salt stress. This study represents the first comprehensive transcriptome-based gene expression profiling under salt stress in radish. The outcomes of this study could facilitate further dissecting the molecular mechanism underlying salt stress response and provide a valuable platform for further genetic improvement of salt tolerance in radish breeding programs.


Plant Cell Rep


Plant cell reports


Sun X,Xu L,Wang Y,Luo X,Zhu X,Kinuthia KB,Nie S,Feng H,Li C,Liu L




Has Abstract


2016-02-01 00:00:00














  • Callus formation from protoplasts of a sugarbeet cell suspension culture.

    abstract::Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protopla...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Szabados L,Gaggero C

    更新日期:1985-08-01 00:00:00

  • Reduced glutathione promotes callus growth and shoot development in a shoot tip culture of apple root stock M26.

    abstract::Reduced glutathione (GSH) was applied to prevent browning in shoot tip explants of apple (Malus pumila Mill.). Development was compared between shoot tips treated either by (a) dipping into 0.1 mM GSH solution prior to culture (dip treatment), (b) dipping into the GSH solution and transferring to a medium containing 0...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Nomura K,Matsumoto S,Masuda K,Inoue M

    更新日期:1998-05-01 00:00:00

  • Quantitative trait loci associated with androgenic responsiveness in triticale (×Triticosecale Wittm.) anther culture.

    abstract::Quantitative trait loci (QTLs) associated with androgenic responsiveness in triticale were analyzed using a population of 90 DH lines derived from the F1 cross between inbred line 'Saka 3006' and cv. 'Modus', which was used in a number of earlier studies on molecular mapping in this crop. Using Windows QTL Cartographe...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Krzewska M,Czyczyło-Mysza I,Dubas E,Gołębiowska-Pikania G,Golemiec E,Stojałowski S,Chrupek M,Zur I

    更新日期:2012-11-01 00:00:00

  • Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans.

    abstract:KEY MESSAGE:We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestans infection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different sig...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Fantino E,Segretin ME,Santin F,Mirkin FG,Ulloa RM

    更新日期:2017-07-01 00:00:00

  • Endogenous jasmonic and salicylic acids levels in the Cd-hyperaccumulator Noccaea (Thlaspi) praecox exposed to fungal infection and/or mechanical stress.

    abstract:KEY MESSAGE:Sensitivity to Erysiphe in Noccaea praecox with low metal supply is related to the failure in enhancing SA. Cadmium protects against fungal-infection by direct toxicity and/or enhanced fungal-induced JA signaling. Metal-based defense against biotic stress is an attractive hypothesis on evolutionary advantag...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Llugany M,Martin SR,Barceló J,Poschenrieder C

    更新日期:2013-08-01 00:00:00

  • Characterization of a chalcone synthase (CHS) flower-specific promoter from Lilium orential 'Sorbonne'.

    abstract::The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Liu Y,Lou Q,Xu W,Xin Y,Bassett C,Wang Y

    更新日期:2011-12-01 00:00:00

  • Genetic relatedness of Portuguese almond cultivars assessed by RAPD and ISSR markers.

    abstract::Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for th...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Martins M,Tenreiro R,Oliveira MM

    更新日期:2003-08-01 00:00:00

  • In planta 2,3,5 truodobenzoic acid treatment promotes high frequency and routine in vitro regeneration of sugarbeet (Beta vulgaris L.) plants.

    abstract::The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Roussy I,Dubois F,Sangwan RS,Sangwan-Norreel BS

    更新日期:1996-12-01 00:00:00

  • Phytochelatin accumulation and stress tolerance in tomato cells exposed to cadmium.

    abstract::Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gupta SC,Goldsbrough PB

    更新日期:1990-12-01 00:00:00

  • Good manufacturing practices production of a purification-free oral cholera vaccine expressed in transgenic rice plants.

    abstract:KEY MESSAGE:The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Despite our knowledge of their advantages, plant-based vaccines remain unavailable ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Kashima K,Yuki Y,Mejima M,Kurokawa S,Suzuki Y,Minakawa S,Takeyama N,Fukuyama Y,Azegami T,Tanimoto T,Kuroda M,Tamura M,Gomi Y,Kiyono H

    更新日期:2016-03-01 00:00:00

  • Effect of promoter-leader sequences on transient expression of reporter gene chimeras biolistically transferred into sugarbeet (Beta vulgaris) suspension cells.

    abstract::Chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells....

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ingersoll JC,Heutte TM,Owens LD

    更新日期:1996-08-01 00:00:00

  • Development of a phosphomannose isomerase-based Agrobacterium-mediated transformation system for chickpea (Cicer arietinum L.).

    abstract::To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Patil G,Deokar A,Jain PK,Thengane RJ,Srinivasan R

    更新日期:2009-11-01 00:00:00

  • Genotype-specific heat shock proteins in two maize inbreds.

    abstract::Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Jorgensen JA,Weng J,Ho TH,Nguyen HT

    更新日期:1992-10-01 00:00:00

  • Effects of cadmium stress on the antioxidant system and chlorophyll fluorescence characteristics of two Taxodium clones.

    abstract:KEY MESSAGE:The T.118 and T.406 seedlings showed strong adaptability under Cd concentrations ≤ 50 µM. The mechanisms of photoprotection in T.118 and T.406 differed in high-Cd concentrations. To explore the physiological response characteristics of Taxodium hybrids to cadmium (Cd) stress and provide basis for screening ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ma YL,Wang HF,Wang P,Yu CG,Luo SQ,Zhang YF,Xie YF

    更新日期:2018-11-01 00:00:00

  • Genetic modification in Malaysia and India: current regulatory framework and the special case of non-transformative RNAi in agriculture.

    abstract::Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审


    authors: Darsan Singh JK,Mat Jalaluddin NS,Sanan-Mishra N,Harikrishna JA

    更新日期:2019-12-01 00:00:00

  • Genotypic and developmental regulation of transient expression of a reporter gene in soybean zygotic cotyledons.

    abstract::Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Moore PJ,Moore AJ,Collins GB

    更新日期:1994-07-01 00:00:00

  • Production of pineapple plants in vitro.

    abstract::In vitro culture of pineapple (Ananas comosus) was studied to determine the efficiency of axillary bud culture for rapid propagation of several cultivars. The technique used maximizes the success rate of various steps in the production of pineapple plants. Rapid mass multiplication of plantlets started 9 months after ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Dewald MG,Moore GA,Sherman WB,Evans MH

    更新日期:1988-12-01 00:00:00

  • Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets.

    abstract::Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitab...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Shoyama Y,Zhu XX,Nakai R,Shiraishi S,Kohda H

    更新日期:1997-04-01 00:00:00

  • Regeneration of plants from mesophyll protoplasts of the wild crucifer Eruca sativa Lam.

    abstract::Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Reg...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Sikdar SR,Chatterjee G,Das S,Sen SK

    更新日期:1987-12-01 00:00:00

  • Shoot regeneration from cultured root explants of spinach (Spinacia oleracea L.): a system for Agrobacterium transformation.

    abstract::A reliable plant regeneration system is described for the production of adventitious shoots from root explants of spinach. Explants from roots of axenic shoots and roots induced on cultured hypocotyl explants were used for adventitious shoot induction. Explants from apical, middle and basal root regions were incubated...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Knoll KA,Short KC,Curtis IS,Power JB,Davey MR

    更新日期:1997-12-01 00:00:00

  • In vitro storage of cedar shoot cultures under minimal growth conditions.

    abstract::We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Renau-Morata B,Arrillaga I,Segura J

    更新日期:2006-07-01 00:00:00

  • Alfalfa MsCBL4 enhances calcium metabolism but not sodium transport in transgenic tobacco under salt and saline-alkali stress.

    abstract:KEY MESSAGE:MsCBL4 expression in tobacco enhanced its salt and saline-alkali stress tolerance by regulating calcium accumulation in roots, indicating the important role of calcium metabolism in plant saline-alkali stress tolerance The calcineurin B-like (CBL) family of proteins play important roles in plant abiotic str...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: An Y,Yang XX,Zhang L,Zhang J,Du B,Yao L,Li XT,Guo C

    更新日期:2020-08-01 00:00:00

  • Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus.

    abstract::Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootsto...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Zanek MC,Reyes CA,Cervera M,Peña EJ,Velázquez K,Costa N,Plata MI,Grau O,Peña L,García ML

    更新日期:2008-01-01 00:00:00

  • Fertile indica and japonica rice plants regenerated from protoplasts isolated from embryogenic haploid suspension cultures.

    abstract::A system to regenerate fertile rice (Oryza sativa L.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. Green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiati...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Su RC,Rudert ML,Hodges TK

    更新日期:1992-12-01 00:00:00

  • Heterologous expression of a novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene, ZjGRP, caused salt sensitivity in Arabidopsis.

    abstract:KEY MESSAGE:A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic Arabidopsis. Glycine-rich RNA-binding proteins (GRPs) play crucial roles in diverse plant developmental processes. However, the mechanisms and func...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Teng K,Tan P,Xiao G,Han L,Chang Z,Chao Y

    更新日期:2017-01-01 00:00:00

  • Genetic transformation ofRhododendron byAgrobacterium tumefaciens.

    abstract::TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin v...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ueno K,Fukunaga Y,Arisumi K

    更新日期:1996-11-01 00:00:00

  • Influence of light intensity and selection scheme on regeneration time of transgenic flax plants.

    abstract::This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Caillot S,Rosiau E,Laplace C,Thomasset B

    更新日期:2009-03-01 00:00:00

  • Plant regeneration fromArabidopsis thaliana protoplasts.

    abstract::Arabidopsis thaliana protoplasts, isolated from a cell suspension, regenerated cell walls and then exhibited sustained cell divisions. Within 10 days after isolation as many as 40% of the protoplast-derived cells divided. Protoplast-derived calli exhibited a wide range of developmental responses, including apparent em...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Ford KG

    更新日期:1990-01-01 00:00:00

  • Plant regeneration from leaf explants of Glycine clandestina Wendl.

    abstract::Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hymowitz T,Chalmers NL,Costanza SH,Saam MM

    更新日期:1986-06-01 00:00:00

  • Two EST-derived marker systems for cultivar identification in tree peony.

    abstract::Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Zhang JJ,Shu QY,Liu ZA,Ren HX,Wang LS,De Keyser E

    更新日期:2012-02-01 00:00:00