Abstract:
:Phenylalanine ammonia-lyase [PAL, EC 4.3.1.24 (formerly EC 4.3.1.5)], functions in the plant phenylpropanoid biosynthetic pathway to deaminate the amino acid L-phenylalanine forming trans-cinnamic acid and ammonia. The human inherited metabolic disorder phenylketonuria (PKU) is characterized by an inability of individuals to metabolize phenylalanine. Toward the development of a plant-PAL based therapeutic for the treatment of this disorder, a comparative analysis of PAL activities within various members of the Poaceae was undertaken. This led to the identification of a Zea mays cultivar, Japanese Striped corn with very high levels of PAL specific activity in seedling tissues. The root tissues of this corn variety contain greater levels of PAL gene transcripts and PAL activities, compared to those of the shoot tissues, and are intensely colored due to the accumulation of anthocyanin pigments. PAL activities in the root tissues of young seedlings of another corn variety that lacked root anthocyanins (Indian Blue corn) were generally 30-50% lower than those of Japanese Striped corn seedlings at equivalent growth stages. In general, various stress or hormonal treatments led to minimal changes in PAL specific activity of maize tissues, as compared to controls. The PAL enzymes of Japanese Striped corn root tissues are robust; roots retained 90% of their PAL activity after freeze-drying and >50% activity after freeze-drying and a subsequent 15-week storage at 4 degrees C. This work serves as a prelude to the formulation of a dietary supplement for treatment of PKU based on preserved edible cereal root tissues with high levels of intrinsic PAL activity.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
McInnis S,Clemens S,Kermode ARdoi
10.1007/s00299-008-0650-6subject
Has Abstractpub_date
2009-03-01 00:00:00pages
503-15issue
3eissn
0721-7714issn
1432-203Xjournal_volume
28pub_type
杂志文章abstract::Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. 'Russet Burbank'. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233028
更新日期:1991-05-01 00:00:00
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01890875
更新日期:1996-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00241936
更新日期:1993-01-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000288
更新日期:2001-01-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2010-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1990-06-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2005-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1725-1
更新日期:2015-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0519-8
更新日期:2008-05-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239891
更新日期:1994-01-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2217-x
更新日期:2018-01-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269518
更新日期:1988-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00571305
更新日期:1985-05-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270102
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1007/s00299-010-0863-3
更新日期:2010-07-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233315
更新日期:1994-02-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272760
更新日期:1988-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1761-5
更新日期:2015-06-01 00:00:00
abstract::Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus dev...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232932
更新日期:1994-08-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000276
更新日期:2000-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234368
更新日期:1992-07-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF02441005
更新日期:1984-06-01 00:00:00
abstract::The Golgi apparatus in plants is organized as a multitude of individual stacks that are motile in the cytoplasm and in close association with the endoplasmic reticulum (ER) (Boevink et al. in Plant J 15:441-447, 1998). These stacks operate as a sorting centre for cargo molecules, providing modification and redirection...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-007-0390-z
更新日期:2007-09-01 00:00:00