Enzymatic synthesis of 6'-deoxychalcone in cultured Glycyrrhiza echinata cells.

abstract：:The effects of NaCl, feeder cells and the embedding of protoplasts in calcium alginate have been investigated in an attempt to improve culture conditions of recalcitrant sugar beet (Beta vulgaris L.) mesophyll protoplasts. While the use of NaCl in all instances proved detrimental to protoplast development, the other t...

journal_title：Plant cell reports

authors： Hall RD,Pedersen C,Krens FA

更新日期：1993-04-01 00:00:00

abstract：:Medicago sativa lines with a high incidence of regeneration were established as suspension cultures and used to select for NaCl tolerant lines. Attempts were then made to regenerate plants from these lines. Regeneration was severely depressed in NaCl tolerant calli and the only plants that were successfully regenerate...

journal_title：Plant cell reports

authors： Smith MK,McComb JA

更新日期：1983-06-01 00:00:00

abstract：:Yucca valida is an important potential source of steroidal saponins closely related to Yucca schidigera, the species that is commercially exploited from the wild as a source of steroidal extracts. Neither of the species has been domesticated mainly because of their slow growth and long life span before harvesting. Her...

journal_title：Plant cell reports

authors： Arce-Montoya M,Rodríguez-Alvarez M,Hernández-González JA,Robert ML

更新日期：2006-08-01 00:00:00

abstract：:Accurate and timely detection of transgene copy number in sugarcane is currently hampered by the requirement to use Southern blotting, needing relatively large amounts of genomic DNA and, therefore, the continued growth and maintenance of bulky plants in containment glasshouses. In addition, the sugarcane genome is bo...

journal_title：Plant cell reports

authors： Casu RE,Selivanova A,Perroux JM

更新日期：2012-01-01 00:00:00

abstract：:In maize, Mutator transposable elements are either active or silenced within the genome. In response to environmental stress, silenced Mutator elements could be reactivated, leading to changes in genome structure and gene function. However, there is no direct experimental evidence linking environmental stress and Muta...

journal_title：Plant cell reports

authors： Qian Y,Cheng X,Liu Y,Jiang H,Zhu S,Cheng B

更新日期：2010-12-01 00:00:00

abstract：:The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...

journal_title：Plant cell reports

authors： Tian L,Séguin A,Charest PJ

更新日期：1997-02-01 00:00:00

abstract：:Hypocotyl explants of three leguminous forest tree species, Albizia amara, A. lucida and A. richardiana, have differentiated shoot buds on B5 basal medium. Maximum number of shoots per explant developed on basal medium augmented with 2,4-D (0.1 μM) in A. amara (2) and BA (10 μM) for both A. lucida (2) and A. richardia...

journal_title：Plant cell reports

authors： Tomar UK,Gupta SC

更新日期：1988-10-01 00:00:00

abstract：:Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...

journal_title：Plant cell reports

authors： Gupta SC,Goldsbrough PB

更新日期：1990-12-01 00:00:00

abstract：:Microcallus (more than 60 cells) formation was obtained from leaf mesophyll protoplasts of 6 species and varieties in the genus Actinidia Lindl. (kiwifruit). The best results were achieved by using liquid over agarose culture for A. arguta var. arguta, liquid and agarose disc type culture for A. arguta var. purpurea, ...

journal_title：Plant cell reports

authors： Xiao XG,Hirsch AM

更新日期：1996-09-01 00:00:00

abstract：:Callus cultures have been established from root explants of aseptically-grown Podophyllum hexandrum seedlings. A fully defined medium based on Gamborg's B5 salts supplemented with 2/4-dichlorophenoxyacetic acid, gibberellic acid and 6-benzylaminopurine was effective for both initiation and sustained growth of callus t...

journal_title：Plant cell reports

authors： Heyenga AG,Lucas JA,Dewick PM

更新日期：1990-11-01 00:00:00

abstract：KEY MESSAGE:The radish WRKY gene family was genome-widely identified and played critical roles in response to multiple abiotic stresses. The WRKY is among the largest transcription factors (TFs) associated with multiple biological activities for plant survival, including control response mechanisms against abiotic stre...

journal_title：Plant cell reports

authors： Karanja BK,Fan L,Xu L,Wang Y,Zhu X,Tang M,Wang R,Zhang F,Muleke EM,Liu L

更新日期：2017-11-01 00:00:00

abstract：KEY MESSAGE:This study established a rapid method for the gene expression analysis in potato tubers. The use of microtubers would be useful for primary evaluation of tuber-expressed genes. In the development of transgenic potato or of potato with other genome modifications (e.g., genome editing or RNA-directed DNA meth...

journal_title：Plant cell reports

authors： Wakasa Y,Kasai A,Yamazaki M,Tabei Y,Tsuyama M,Igarashi T,Okazaki T,Yamamoto K,Fujihara H,Kanno A,Noro O,Harada T,Akada S

更新日期：2020-11-01 00:00:00

abstract：:Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines d...

journal_title：Plant cell reports

authors： Evans DA,Gamborg OL

更新日期：1982-04-01 00:00:00

abstract：:Salt tolerant cell lines have been selected from Medicago sativa, by a single step selection process on tissue culture medium containing 1% NaCl. Plants regenerated from these lines show improved salt tolerance compared to parent plants. The regenerated plants are vigorous, have flowered and are self fertile. The cell...

journal_title：Plant cell reports

authors： Winicov I

更新日期：1991-12-01 00:00:00

abstract：:The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were amplified from mungbean (Vigna radiata) genome using degenerate primers, cloned and sequenced. Among these 34% and 65% of respective clones of copia and gypsy RT sequences pos...

journal_title：Plant cell reports

authors： Dixit A,Ma KH,Yu JW,Cho EG,Park YJ

更新日期：2006-03-01 00:00:00

abstract：:We report the production of taxadiene by transformation of N. benthamiana with a taxadiene synthase gene. The production was significantly increased by an elicitor treatment or metabolic pathway shunting. Paclitaxel (Taxol(®)) was first isolated from the bark of the pacific yew tree as an anticancer agent and has been...

journal_title：Plant cell reports

authors： Hasan MM,Kim HS,Jeon JH,Kim SH,Moon B,Song JY,Shim SH,Baek KH

更新日期：2014-06-01 00:00:00

abstract：:Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protopla...

journal_title：Plant cell reports

authors： Szabados L,Gaggero C

更新日期：1985-08-01 00:00:00

abstract：:Mitogen-activated protein kinase (MAPK) signaling cascades are evolutionarily conserved fundamental signal transduction pathways. A MAPK cascade consists of many distinct MAPKKK-MAPKK-MAPK modules linked to various upstream receptors and downstream targets through sequential phosphorylation and activation of the casca...

journal_title：Plant cell reports

authors： Singh R,Jwa NS

更新日期：2013-06-01 00:00:00

abstract：:A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Avera...

journal_title：Plant cell reports

authors： Sigareva M,Spivey R,Willits MG,Kramer CM,Chang YF

更新日期：2004-10-01 00:00:00

abstract：:A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protop...

journal_title：Plant cell reports

authors： Edwards ES,Roux SJ

更新日期：1998-06-01 00:00:00

abstract：:The purpose of this study was to develop a new micropropagation system for Tylophora indica, an important medicinal plant in India, using root explants as starting material. Root explants cultured on MS medium supplemented with 6-benzyladenine (BA) or 2-isopentyladenine (2iP) produced organogenic nodular meristemoids ...

journal_title：Plant cell reports

authors： Chaudhuri KN,Ghosh B,Jha S

更新日期：2004-05-01 00:00:00

abstract：:The activity and activation potential of polyphenoloxidase (PPO, E.C. 1.10.3.1.) of tissue from shoot tips, adult leaves and embryogenic and non-embryogenic cell suspension cultures of Euphorbia pulcherrima was investigated using an oxygen probe technique. PPO derived from differentiated in vivo plant tissue (shoot ti...

journal_title：Plant cell reports

authors： Grotkass C,Lieberei R,Preil W

更新日期：1995-04-01 00:00:00

abstract：:An Arabidopsis mutant line T90, exhibiting a stem-specific and wound-responsive GUS expression was identified from a population of Arabidopsis thaliana tagged with a promoterless β-glucuronidase (GUS) in the T-DNA. Sequence flanking the insertion from the right border was amplified by TAIL PCR and cloned. The insertio...

journal_title：Plant cell reports

authors： Gupta NC,Jain PK,Bhat SR,Srinivasan R

更新日期：2012-05-01 00:00:00

abstract：:Twenty five clones from a carrot genomic library were used as probes to detect variations in the 5-methyl-cytosine pattern during somatic embryogenesis. The majority of them evidenced an invariant pattern. With two clones however, differences were found between the adulttype DNA pattern and the embryonic one. The leve...

journal_title：Plant cell reports

authors： Vergara R,Verde F,Pitto L,Lo Schiavo F,Terzi M

更新日期：1990-05-01 00:00:00

abstract：:Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...

journal_title：Plant cell reports

authors： Kim YH,Woloshuk CP,Cho EH,Bae JM,Song YS,Huh GH

更新日期：2007-04-01 00:00:00

abstract：:Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Fo...

journal_title：Plant cell reports

authors： Patat-Ochatt EM,Boccon-Gibod J,Duron M,Ochatt SJ

更新日期：1993-01-01 00:00:00

abstract：:Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). How...

journal_title：Plant cell reports

authors： Ellis JS,Jennings AC,Edwards LA,Mavandad M,Lamb CJ,Dixon RA

更新日期：1989-12-01 00:00:00

abstract：:The interconversion between 5 to 8-membered cycloalkanones and the corresponding cycloalkanols by a cell suspension culture of Nicotiana tabacum was studied. It was found that the interconversion is at equilibrium and the balance in the equilibrium depends on the number of carbon atoms in the carbocyclic ring of the s...

journal_title：Plant cell reports

authors： Suga T,Hamada H,Hirata T

更新日期：1983-04-01 00:00:00

abstract：:A simple and reliable method was developed for isolating pollen nuclei from Brassica napus and Triticum aestivum for DNA analysis using flow cytometry. The nuclei were released from pollen by ultrasonic treatment. The isolated nuclei following filtration through nylon mesh and a purification procedure were suitable fo...

journal_title：Plant cell reports

authors： Pan G,Zhou Y,Fowke LC,Wang H

更新日期：2004-10-01 00:00:00

abstract：:Root vigor is an important trait for the growth of terrestrial plants, especially in water-deficit environments. Although deserts plants are known for their highly developed root architecture, the molecular mechanism responsible for this trait has not been determined. Here we established an efficient protocol for the ...

journal_title：Plant cell reports

authors： Kajikawa M,Morikawa K,Abe Y,Yokota A,Akashi K

更新日期：2010-07-01 00:00:00