Reversible variations in the methylation pattern of carrot DNA during somatic embryogenesis.

abstract：:Novel and efficient protocols for plant regeneration and genetic transformation from longitudinally-halved cotyledons ofin vitro raised seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation withAgrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgen...

journal_title：Plant cell reports

authors： Fári M,Nagy I,Csányi M,Mitykó J,Andrásfalvy A

更新日期：1995-01-01 00:00:00

abstract：:This article describes conditions to optimize the yield of viable protoplasts from callus tissue of Asparagus densiflorus cv. Sprengeri and their subsequent regeneration into plantlets. Callus tissue was initiated by culturing spear sections (5-7 mm) on Murashige and Skoog (MS) medium supplemented with 0.8% (wt/vol) B...

journal_title：Plant cell reports

authors： Benmoussa M,Mukhopadhyay S,Desjardins Y

更新日期：1997-12-01 00:00:00

abstract：KEY MESSAGE:Transcriptome-based gene expression analysis identifies many critical salt-responsive genes in radish and facilitates further dissecting the molecular mechanism underlying salt stress response. Salt stress severely impacts plant growth and development. Radish, a moderately salt-sensitive vegetable crop, has...

journal_title：Plant cell reports

authors： Sun X,Xu L,Wang Y,Luo X,Zhu X,Kinuthia KB,Nie S,Feng H,Li C,Liu L

更新日期：2016-02-01 00:00:00

abstract：KEY MESSAGE:A R2R3-MYB transcription factor EsAN2 was isolated from Epimedium sagittatum and functionally characterized to regulate the anthocyanin biosynthetic pathway. Epimedium plants are used widely both as traditional Chinese medicinal herbs and ornamental perennials. Anthocyanins, acting as major contributors to ...

journal_title：Plant cell reports

authors： Huang W,Khaldun AB,Lv H,Du L,Zhang C,Wang Y

更新日期：2016-04-01 00:00:00

abstract：:The objective of this study was to correlate the time that DNA synthesis first occurs in haploid microspores of barley with cell cycle and plant morphological stages and to subsequently assess the influence of pretreatments on DNA synthesis at different stages of microspore development. Spikes with microspores in earl...

journal_title：Plant cell reports

authors： Shim YS,Kasha KJ

更新日期：2003-07-01 00:00:00

abstract：:We report the production of taxadiene by transformation of N. benthamiana with a taxadiene synthase gene. The production was significantly increased by an elicitor treatment or metabolic pathway shunting. Paclitaxel (Taxol(®)) was first isolated from the bark of the pacific yew tree as an anticancer agent and has been...

journal_title：Plant cell reports

authors： Hasan MM,Kim HS,Jeon JH,Kim SH,Moon B,Song JY,Shim SH,Baek KH

更新日期：2014-06-01 00:00:00

abstract：KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...

journal_title：Plant cell reports

authors： Coronel CJ,González AI,Ruiz ML,Polanco C

更新日期：2018-01-01 00:00:00

abstract：:Plants were regenerated from leafy spurge (Euphorbia esula L.) cell suspensions obtained from stem callus. A North Dakota accession was highly regenerable, but two accessions from Oregon and Austria formed only a few plantlets. Organogenesis occurred in media without growth regulators, under fluorescent lights (30 to ...

journal_title：Plant cell reports

authors： Davis DG,Olson PA,Stolzenberg RL

更新日期：1988-06-01 00:00:00

abstract：:The human Bcl-xL gene was transformed into peanut cultivar Georgia Green via microprojectile bombardment. Following selection on hygromycin-containing medium and regeneration, eighty hygromycin-resistant callus clusters were recovered. Southern blot analysis of ten fertile lines revealed multiple insertions of the Bcl...

journal_title：Plant cell reports

authors： Chu Y,Deng XY,Faustinelli P,Ozias-Akins P

更新日期：2008-01-01 00:00:00

abstract：:Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or ligh...

journal_title：Plant cell reports

authors： Mizukami H,Tomita K,Ohashi H

更新日期：1989-12-01 00:00:00

abstract：:Arabinogalactan proteins (AGPs) are important proteoglycans regulating somatic embryogenesis in diverse plant species. Embryogenic cells of somatic embryos are covered by special extracellular cell wall layer called extracellular surface matrix network (ECMSN) at their early developmental stages. Here we show that hig...

journal_title：Plant cell reports

authors： Samaj J,Salaj T,Matúsová R,Salaj J,Takác T,Samajová O,Volkmann D

更新日期：2008-02-01 00:00:00

abstract：:A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...

journal_title：Plant cell reports

authors： Gupta V,Agnihotri A,Jagannathan V

更新日期：1990-12-01 00:00:00

abstract：:The introgression of new desirable characters is very necessary in common bean (Phaseolus vulgaris L.)Interspecific hybridization of P. vulgaris L. with P. angustissimus A. Gray, a wild species with narrow leaves, could be successfully achieved for the first time using embryo culture. Sixteen to twenty-three-day old e...

journal_title：Plant cell reports

authors： Belivanis T,Doré C

更新日期：1986-10-01 00:00:00

abstract：:Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes. The transformed cultures of ginseng could be subcultured as an axenic root culture in the absence of phytohormones, and grew with extensive lateral branches more rapidly t...

journal_title：Plant cell reports

authors： Yoshikawa T,Furuya T

更新日期：1987-12-01 00:00:00

abstract：KEY MESSAGE:A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineeri...

journal_title：Plant cell reports

authors： Li J,Xu RF,Qin RY,Ma H,Li H,Zhang YP,Li L,Wei PC,Yang JB

更新日期：2014-10-01 00:00:00

abstract：:Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobact...

journal_title：Plant cell reports

authors： Peng RH,Yao QH,Xiong AS,Cheng ZM,Li Y

更新日期：2006-03-01 00:00:00

abstract：:The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...

journal_title：Plant cell reports

authors： Meyer G,Bliedung H,Kloppstech K

更新日期：1983-02-01 00:00:00

abstract：:Genistein, 4',5,7-trihydroxyisoflavone, is an isoflavonoid compound predominantly restricted to legumes and known to possess phyto-oestrogenic and antioxidative activities. The key enzyme that redirects phenylpropanoid pathway intermediates from flavonoids to isoflavonoids is the isoflavone synthase (IFS). Brassica na...

journal_title：Plant cell reports

authors： Li X,Qin JC,Wang QY,Wu X,Lang CY,Pan HY,Gruber MY,Gao MJ

更新日期：2011-08-01 00:00:00

abstract：KEY MESSAGE:Red alga, Gracilariopsis chorda, contains seven carbonic anhydrases that can be grouped into α-, β- and γ-classes. Carbonic anhydrases (CAHs) are metalloenzymes that catalyze the reversible hydration of CO2. These enzymes are present in all living organisms and play roles in various cellular processes, incl...

journal_title：Plant cell reports

authors： Razzak MA,Lee J,Lee DW,Kim JH,Yoon HS,Hwang I

更新日期：2019-02-01 00:00:00

abstract：:A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protop...

journal_title：Plant cell reports

authors： Edwards ES,Roux SJ

更新日期：1998-06-01 00:00:00

abstract：:Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1-4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophen...

journal_title：Plant cell reports

authors： Fereol L,Chovelon V,Causse S,Triaire D,Arnault I,Auger J,Kahane R

更新日期：2005-08-01 00:00:00

abstract：:Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, sug...

journal_title：Plant cell reports

authors： Rodríguez-Concepción M,Gómez MD,Beltrán JP

更新日期：1996-04-01 00:00:00

abstract：KEY MESSAGE:The recovery of non-functional-enhanced green fluorescence protein can be used as indicator to facilitate the identification of mutants generated by CRISPR/Cas9. The CRISPR/Cas9 system is a powerful tool for genome editing and it has been employed to knock out genes of interest in multiple plant species. Id...

journal_title：Plant cell reports

authors： Ren C,Guo Y,Gathunga EK,Duan W,Li S,Liang Z

更新日期：2019-12-01 00:00:00

abstract：:A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Avera...

journal_title：Plant cell reports

authors： Sigareva M,Spivey R,Willits MG,Kramer CM,Chang YF

更新日期：2004-10-01 00:00:00

abstract：:DNA transfer into zygotic embryos of Eucalyptus globulus by microprojectile bombardment was studied with two devices: a gunpowder apparatus and a compressed-helium system. Using, as a test, the transient expression of a reporter gene, we optimized the physical and biological conditions of bombardment. Six-day-old cult...

journal_title：Plant cell reports

authors： Rochange F,Serrano L,Marque C,Teuliéres C,Boudet AM

更新日期：1995-07-01 00:00:00

abstract：KEY MESSAGE:Thirty genes involved in GA and ABA metabolism and signalling were identified, and the expression profiles indicated that they play crucial roles in the bud activity-dormancy transition in tea plants. Gibberellin (GA) and abscisic acid (ABA) are fundamental phytohormones that extensively regulate plant grow...

journal_title：Plant cell reports

authors： Yue C,Cao H,Hao X,Zeng J,Qian W,Guo Y,Ye N,Yang Y,Wang X

更新日期：2018-03-01 00:00:00

abstract：:Transgenic herbicide tolerant Acacia sinuata plants were produced by transformation with the bar gene conferring phosphinothricin resistance. Precultured hypocotyl explants were infected with Agrobacterium tumefaciens strain EHA105 in the presence of 100 microM acetosyringone and shoots regenerated on MS (Murashige an...

journal_title：Plant cell reports

authors： Vengadesan G,Amutha S,Muruganantham M,Anand RP,Ganapathi A

更新日期：2006-11-01 00:00:00

abstract：:Leaf mesophyll protoplasts isolated from pea (Pisum sativum L.) genotypes Century and PI244253 showed transient expression of β-glucuronidase (GUS) when electroporated with plasmid DNA containing various promoter-leader sequence constructs driving the GUS gene. The optimum conditions for transient expression were: usi...

journal_title：Plant cell reports

authors： Hobbs SL,Jackson JA,Baliski DS,Delong CM,Mahon JD

更新日期：1990-06-01 00:00:00

abstract：KEY MESSAGE:Marker-free transgenic plants can be generated with high efficiency by using the Cre/ lox P self-excision system controlled by the pollen- and embryo-specific Arabidopsis DLL promoter. In this work, we aimed to study the feasibility of using the pollen- and embryo-specific DLL promoter of the At4g16160 gene...

journal_title：Plant cell reports

authors： Polóniová Z,Jopčík M,Matušíková I,Libantová J,Moravčíková J

更新日期：2015-03-01 00:00:00

abstract：:The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of n...

journal_title：Plant cell reports

authors： Khan BM,Dwivedi UN,Rawal SK,Mascarenhas AF

更新日期：1984-08-01 00:00:00