Organogenesis in cell cultures of leafy spurge (Euphorbiaceae) accessions from Europe and North America.

Abstract:

:Plants were regenerated from leafy spurge (Euphorbia esula L.) cell suspensions obtained from stem callus. A North Dakota accession was highly regenerable, but two accessions from Oregon and Austria formed only a few plantlets. Organogenesis occurred in media without growth regulators, under fluorescent lights (30 to 90 μE m(-2) s(-1), 14 h photoperiod). Organogenesis was greatest in larger size clumps subcultured during maximum cell growth into media containing a reduced:oxidized nitrogen ratio of 33:67. Roots formed first and some clumps produced shoots. Organogenic suspension cultures also were initiated from hypocotyl and root segments of germinated seedlings, directly in liquid medium. Plantlets of the North Dakota accession formed in vitro adapted to greenhouse conditions. They were phenotypically similar to the parent plants.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Davis DG,Olson PA,Stolzenberg RL

doi

10.1007/BF00272536

subject

Has Abstract

pub_date

1988-06-01 00:00:00

pages

253-6

issue

4

eissn

0721-7714

issn

1432-203X

journal_volume

7

pub_type

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