Abstract：
:A collection of 29 pathogenesis-related 10 (PR10) genes of Medicago sativa and Medicago truncatula showed that they were almost all obtained from cDNA libraries of tissues undergoing abiotic or biotic stresses. The predicted proteins could be divided into two subclasses, PR10.1 and PR10.2, but in silico predicted models of their three-dimensional structures revealed that they could be further divided based on size of the hydrophobic internal cavity and number of β-bulges. A comparison of the expression of two highly similar M. sativa subclass PR10.1 genes, MsPR10.1A and MsPR10.1B, predicted to have similar sized hydrophobic internal cavities, but a different number of β-bulges revealed differences in their expression patterns. MsPR10.1A was induced faster than MsPR10.1B by ABA, ethylene, and X. campestris pv. alfalfae, but slower than MsPR10.1B by harvesting and wounding. Unlike MsPR10.1A, MsPR10.1B expression was induced in non-harvested tissues following harvesting, but was not induced by heat treatment. Histochemical observations of Nicotiana benthamiana transformed with 657 bp of the MsPR10.1A promoter fused to the β-glucuronidase (GUS) gene showed that GUS expression was wound-inducible in leaves, which was consistent with MsPR10.1A expression in alfalfa leaves. GUS expression in stems and leaves was mostly in vascular tissue. The MsPR10.1A promoter may be valuable in controlling the expression in vascular tissues and disease resistance.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Bahramnejad B,Goodwin PH,Zhang J,Atnaseo C,Erickson LRdoi
10.1007/s00299-010-0909-6subject
Has Abstractpub_date
2010-11-01 00:00:00pages
1235-50issue
11eissn
0721-7714issn
1432-203Xjournal_volume
29pub_type
杂志文章abstract：:A method has been devised which allows the rapid production of fine suspension cultures of small aggregate size from suspension cultures of large average aggregate size, such as those of Capsicum frutescens. The method, which uses a Waring blender for aseptic homogenisation of cultures, has also been shown to be effec...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269538
更新日期：1988-10-01 00:00:00
abstract：:Cell-wall invertase (CIN) catalyzes the hydrolysis of sucrose into glucose and fructose for the supply of carbohydrates to sink organs via an apoplastic pathway. To study the CIN genes in rice (Oryza sativa L.), we isolated cDNA clones showing amino acid similarity to the plant cell wall invertase proteins from a sear...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0910-z
更新日期：2005-06-01 00:00:00
abstract：:In this paper we describe a simple method to improve the plating efficiency in plant cell cultures.Two-stage plating is used; in the first stage the cells are inoculated at high density in 0.2% agarized culture medium for ten days to facilitate growth; under this condition, each cell produces a single micro-colony tra...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00571305
更新日期：1985-05-01 00:00:00
abstract：:The AtGRP5 gene from Arabidopsis thaliana encodes a glycine-rich protein which has a major activity in protoderm-derived cells and is expressed in cells that undergo the first anatomical modifications leading to somatic embryo development. It has been previously demonstrated that its minimum promoter is 316 bp long in...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0835-7
更新日期：2010-05-01 00:00:00
abstract：:Protoplasts were isolated from seedling roots, hypocotyls, and cotyledons of four cultivars of Helianthus annuus and from leaves of axenic shoot cultures of the wild species H. praecox, H. scaberimus and H. rigidus. Optimal culture conditions were established for the respective protoplast systems, using the agarose be...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269815
更新日期：1986-08-01 00:00:00
abstract：:Additional virulence (vir) genes in Agrobacterium tumefaciens and sonication were investigated for their impact on transformation efficiency in loblolly pine (Pinus taeda L.). Mature zygotic embryos of loblolly pine were co-cultivated with disarmed A. tumefaciens strain EHA105 containing either plasmid vector pCAMBIA1...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-002-0550-0
更新日期：2003-02-01 00:00:00
abstract：:Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234368
更新日期：1992-07-01 00:00:00
abstract：KEY MESSAGE:Induced mutations in the waxy locus in rice endosperm did not abolish GBSS activity completely. Compensatory mechanisms in endosperm and leaves caused a major reprogramming of the starch biosynthetic machinery. The mutation of genes in the starch biosynthesis pathway has a profound effect on starch quality ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02388-z
更新日期：2019-03-01 00:00:00
abstract：KEY MESSAGE:This study established a rapid method for the gene expression analysis in potato tubers. The use of microtubers would be useful for primary evaluation of tuber-expressed genes. In the development of transgenic potato or of potato with other genome modifications (e.g., genome editing or RNA-directed DNA meth...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02572-6
更新日期：2020-11-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234589
更新日期：1991-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1106-y
更新日期：2011-10-01 00:00:00
abstract：:Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootsto...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0422-8
更新日期：2008-01-01 00:00:00
abstract：:Shoot cultures ofAtractylodes lancea DC. (Compositae) have been established by inoculating the flower bud on Linsmaier-Skoog's medium supplemented with 10(-5) M naphthaleneacetic acid and 10(-5) M 6-benzyl-aminopurine. Shoots were multiplied on a medium containing 10(-6) M 6-benzylaminopurine. Propagated shoots rooted...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF02441005
更新日期：1984-06-01 00:00:00
abstract：:The steroidal hormone brassinosteroids (BRs) play important roles in plant growth and development. Genetic, genomic and proteomic studies in Arabidopsis have identified major BR signaling components and elucidated the signal transduction pathway from the cell surface receptor kinase BRI1 to the BES1/BZR1 family of tra...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1438-x
更新日期：2013-07-01 00:00:00
abstract：:Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium pH on strain virulence. Tumours were incited on all dico...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235354
更新日期：1991-04-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0657-z
更新日期：2009-03-01 00:00:00
abstract：KEY MESSAGE:Prolamin-GFP fusion proteins, expressed under the control of native prolamin promoters, were localized in specific layers of PB-Is. Prolamin-GFP fusion proteins were gradually digested from outside by pepsin digestion. In rice seed endosperm, protein body type I (PB-I) has a layered structure consisting of ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1960-8
更新日期：2016-06-01 00:00:00
abstract：: An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. T...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050743
更新日期：2000-03-01 00:00:00
abstract：:Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and alpha-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0224-4
更新日期：2007-02-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1331-z
更新日期：2012-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233152
更新日期：1996-08-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050294
更新日期：1997-06-01 00:00:00
abstract：:We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants wa...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050405
更新日期：1998-03-01 00:00:00
abstract：:A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0697-z
更新日期：2009-06-01 00:00:00
abstract：:Callus cultures of Vitis labruscana cv. Concord were initiated from both immature fruit and petiole tissue. Analysis revealed that the acid hydrolyzable precursors to β-damascenone, a compound important to the flavor of most grape species, were present in both callus types. Light and increased sucrose concentration we...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231971
更新日期：1994-05-01 00:00:00
abstract：:Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0250-2
更新日期：2007-04-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-021-02666-9
更新日期：2021-02-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0830-y
更新日期：2004-10-01 00:00:00
abstract：:A procedure to produce fruits from cultured shoot tips of Cucumis sativus L. cultivar 'Marketmore-76' in vitro is described. Four-week-old shoot tips, derived from sterile germinated seedlings on a MS medium, were cultured in a 3.8-1 Mason jar using an automated plant culture system. Tips readily generated roots, leav...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232312
更新日期：1993-11-01 00:00:00
abstract：:Innovations in plant breeding like genome editing methods raised questions about the adequacy of established regulatory policies for plant breeding and biotechnology in view of these new breeding methods and the resulting products. Most countries follow the principle approach that only those plants will be regulated u...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02482-2
更新日期：2020-01-01 00:00:00
