Genetic relatedness of Portuguese almond cultivars assessed by RAPD and ISSR markers.

Abstract:

:Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for their reproducibility and high polymorphism. Out of 124 polymerase chain reaction fragments that were scored, 120 (96.8%) were polymorphic. All the plants could be discriminated and constitute a very heterogeneous group. Five unidentified almond plants found in the region of Foz Côa (north Portugal) and wild almond (P. webbii) from Italy and Spain were also included. Four main groups of plants could be distinguished: P. dulcis cultivars; one Foz Côa plant; P. webbii; and P. persica (outgroup). The segregating Foz Côa plant may represent a feral individual or a hybrid between P. dulcis and P. webbii.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Martins M,Tenreiro R,Oliveira MM

doi

10.1007/s00299-003-0659-9

keywords:

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

71-8

issue

1

eissn

0721-7714

issn

1432-203X

journal_volume

22

pub_type

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