Genetic transformation of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment.

Abstract:

: An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotransferase (nptII) selectable marker gene under the control of a banana bunchy top virus (BBTV) promoter or the CaMV 35S promoter, and either the β-glucuronidase (uidA) reporter gene or BBTV genes under the control of the maize polyubiquitin promoter. Plants were regenerated, under selection with kanamycin, that were co-transformed with nptII and either the uidA or BBTV genes. Molecular characterisation of transformants demonstrated that the transgenes had been stably integrated into the banana genome.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Becker DK,Dugdale B,Smith MK,Harding RM,Dale JL

doi

10.1007/s002990050004

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

229-234

issue

3

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990050004

journal_volume

19

pub_type

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