Abstract:
:To study the developmental transition of chloroplasts from C(3) to C(4) photosynthesis in the terrestrial single-cell C(4) species Bienertia sinuspersici, a regeneration protocol was developed. Stem explant material developed callus either with or without red nodular structures (RNS) when cultured on Murashige-Skoog (MS) salts and vitamins, supplemented with 5 mM phosphate, plus 1 mg L(-1) dichloropenoxy-acetic acid (2,4-D), and 87 mM sucrose (Stage 1 media). Only calli having RNS were able to regenerate plantlets. MS media plus phosphate was used throughout regeneration, with the Stage 2 media containing 2 mg L(-1) 6-benzylaminopurine, 43 mM sucrose and 1.5% soluble starch. Stage 3 media had no hormones or organic sources of carbon, and cultures were grown under ambient (~400 ppm) versus CO(2) enrichment (1.2% CO(2)). When calli without RNS were cultured under Stage 3 conditions with 1.2% CO(2), there was an increase in growth, protein content, and photosystem II yield, while structural and biochemical analyses indicated the cells in the calli had C(3) type photosynthesis. CO(2) enrichment during growth of RNS during Stage 3 had a large effect on regeneration success, increasing efficiency of shoot and root development, size of plantlets, leaf soluble protein, and chlorophyll concentration. Anatomical analysis of plantlets, which developed under 1.2% CO(2), showed leaves developed C(4) type chlorenchyma cells, including expression of key C(4) biochemical enzymes. Increasing salinity in the media, from 0 to 200 mM NaCl, increased tissue osmolality, average plantlet area and regeneration success, but did not affect protein or chlorophyll content.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Rosnow J,Offermann S,Park J,Okita TW,Tarlyn N,Dhingra A,Edwards GEdoi
10.1007/s00299-011-1067-1subject
Has Abstractpub_date
2011-08-01 00:00:00pages
1541-53issue
8eissn
0721-7714issn
1432-203Xjournal_volume
30pub_type
杂志文章abstract::The effect of the polyamine spermidine on the growth of crown gall tumors was determined using the potato disc bioassay. Addition of lmM spermidine resulted in a 30-50% increase in tumor growth. The spermidine effect was found to be biphasic, with lmM being optimal. Closely related polyamines including spermine, as we...
journal_title:Plant cell reports
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272049
更新日期:1989-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1989-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00274134
更新日期:1989-05-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268593
更新日期:1986-10-01 00:00:00
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journal_title:Plant cell reports
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journal_title:Plant cell reports
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2007-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2016-02-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193734
更新日期:1995-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269825
更新日期:1986-08-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章,评审
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更新日期:2010-10-01 00:00:00