Abstract：
:Green fluorescent protein (GFP) has been used widely as a powerful bioluminescent reporter, but its visualization by existing methods in tissues or whole plants and its utilization for high-throughput screening remains challenging in many species. Here, we report a fluorescence image analyzer-based method for GFP detection and its utility for high-throughput screening of transformed plants. Of three detection methods tested, the Typhoon fluorescence scanner was able to detect GFP fluorescence in all Arabidopsis thaliana tissues and apple leaves, while regular fluorescence microscopy detected it only in Arabidopsis flowers and siliques but barely in the leaves of either Arabidopsis or apple. The hand-held UV illumination method failed in all tissues of both species. Additionally, the Typhoon imager was able to detect GFP fluorescence in both green and non-green tissues of Arabidopsis seedlings as well as in imbibed seeds, qualifying it as a high-throughput screening tool, which was further demonstrated by screening the seedlings of primary transformed T(0) seeds. Of the 30,000 germinating Arabidopsis seedlings screened, at least 69 GFP-positive lines were identified, accounting for an approximately 0.23% transformation efficiency. About 14,000 seedlings grown in 16 Petri plates could be screened within an hour, making the screening process significantly more efficient and robust than any other existing high-throughput screening method for transgenic plants.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Hily JM,Liu Zdoi
10.1007/s00299-008-0657-zsubject
Has Abstractpub_date
2009-03-01 00:00:00pages
493-501issue
3eissn
0721-7714issn
1432-203Xjournal_volume
28pub_type
杂志文章abstract：:Plants have their own mechanisms for overcoming various stresses. In cold regions, plants are subject to stress and must enter an inherent dormancy, through several complex mechanisms, if they are to continue to exist. In winter, regulation of tonoplast and plasma membrane aquaporin genes differed in the bud cushions ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0770-7
更新日期：2009-11-01 00:00:00
abstract：KEY MESSAGE:Two lines of Taxus × media hairy roots harbouring or not the TXS transgene demonstrated diverse gene expression and taxane yield during cultivation in PFD-supported two liquid-phase culture system. Two lines of Taxus × media hairy roots were subjected to single or twice-repeated supplementation with methyl ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-018-2351-0
更新日期：2019-01-01 00:00:00
abstract：:A procedure has been developed for the clonal propagation of Picrorhiza kurroa Royle ex Benth. through shoot tip culture. Murashige and Skoog's medium (1962) supplemented with kinetin (3.0 to 5.0 mg/l) supported rapid proliferation of multiple shoots from the explants. Addition of indole-3-acetic acid (1.0 mg/l) to th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269324
更新日期：1988-05-01 00:00:00
abstract：KEY MESSAGE:Transcriptomes of two switchgrass genotypes representing the upland and lowland ecotypes will be key tools in switchgrass genome annotation and biotic and abiotic stress functional genomics. Switchgrass (Panicum virgatum L.) is an important bioenergy feedstock for cellulosic ethanol production. We report ge...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2065-0
更新日期：2017-01-01 00:00:00
abstract：: Embryogenic nucellar callus cultures of different Citrus species and cultivars growing in hormone-free medium were transferred to medium containing either sucrose or glycerol as the only carbohydrate source. Glycerol has been reported to induce further development of Citrus somatic embryos, while in the presence of s...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000280
更新日期：2001-01-01 00:00:00
abstract：KEY MESSAGE:Tetraploid clementine displays mainly tetrasomic inheritance. Genetic structures of 2n SDR and 2 × gametes from DD clementine are complementary and will guides triploids citrus breeding strategies. Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be reco...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1972-4
更新日期：2016-08-01 00:00:00
abstract：:Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234510
更新日期：1994-07-01 00:00:00
abstract：KEY MESSAGE:The gene and cDNA sequence encoding PAL from Chinese medicinal plant Rhus chinensis were cloned and analyzed, furthermore the biochemical properties, kinetic parameters, differential expression and key sites were studied. Rhus chinensis is a well-known Chinese medicinal plant. Phenylalanine ammonia-lyase (P...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1413-6
更新日期：2013-08-01 00:00:00
abstract：:Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and B...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00236268
更新日期：1992-09-01 00:00:00
abstract：:The activity and activation potential of polyphenoloxidase (PPO, E.C. 1.10.3.1.) of tissue from shoot tips, adult leaves and embryogenic and non-embryogenic cell suspension cultures of Euphorbia pulcherrima was investigated using an oxygen probe technique. PPO derived from differentiated in vivo plant tissue (shoot ti...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234048
更新日期：1995-04-01 00:00:00
abstract：:Putrescine, spermidine, and spermine levels during somatic embryogenesis of interior spruce (Picea glauca x Picea engelmannii complex) were quantified On abscisic acid supplemented growth medium putrescine and spermidine levels increased two-fold coinciding with maturation of the early somatic embryos to globular embr...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232981
更新日期：1996-03-01 00:00:00
abstract：:A test system for root regeneration was developed that consists of stem slices of apple shoots (ca. 0.5 mm thick; fresh weight ca. 1 mg). Roots regenerated synchronously without intervening callus formation and without interference with compounds originating from other parts of the plant. Supply of indolebutyric acid ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00237054
更新日期：1993-02-01 00:00:00
abstract：:Cotyledon explants from zygotic embryos of Panax ginseng produced somatic embryos on Murashige and Skoog basal medium without growth regulators. Somatic embryos developed directly from epidermal cells at the cotyledon base. Somatic embryos were always formed from the side of the cotyledon opposite to the one attached ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050331
更新日期：1997-10-01 00:00:00
abstract：:Companion cells are metabolically active and functionally specialized cells that behave as terminals for the transport of materials between phloem and the surrounding tissue. Although previous research has clarified the distinct function of companion cells, it is still largely unknown how plants establish and maintain...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1080-4
更新日期：2011-09-01 00:00:00
abstract：:Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on l...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0080-7
更新日期：2006-05-01 00:00:00
abstract：:The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as thos...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s002990100346
更新日期：2001-07-01 00:00:00
abstract：:Plants have developed diverse molecular mechanisms to resist viruses. RNA silencing plays a dominant role in antiviral defense. Recent studies have correlated plant antiviral silencing to epigenetic modification in genomic DNA and protein by remodeling the expression levels of coding genes. The plant host methylation ...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-019-02414-0
更新日期：2019-09-01 00:00:00
abstract：:Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana ( Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at e...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-002-0537-x
更新日期：2003-03-01 00:00:00
abstract：:Cotyledon explants of muskmelon (Cucumis melo L., cv. Amarillo Oro) seedlings were co-cultivated with disarmed Agrobacterium tumefaciens strain LBA4404 that contained the binary vector plasmid pBI121.1. The T-DNA region of this binary vector contains the Nopaline synthase/neomycin phosphotransferase II (NPTII) chimeri...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00239881
更新日期：1994-01-01 00:00:00
abstract：:Domain of Unknown Function 966 (DUF966) gene family was found in the protein family database, which consisted of seven genes in rice. The proteins encoded by these genes contained one or two highly conserved DUF966 domains. The available data of public microarray databases implied that these genes might play crucial r...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1532-0
更新日期：2014-02-01 00:00:00
abstract：KEY MESSAGE:Sensitivity to Erysiphe in Noccaea praecox with low metal supply is related to the failure in enhancing SA. Cadmium protects against fungal-infection by direct toxicity and/or enhanced fungal-induced JA signaling. Metal-based defense against biotic stress is an attractive hypothesis on evolutionary advantag...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1427-0
更新日期：2013-08-01 00:00:00
abstract：:Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a major disease problem in wheat (Triticum aestivum). Genetic engineering holds significant potential to enhance FHB resistance in wheat. Due to the requirement of screening for FHB resistance on flowers at anthesis, the number of screens carried...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0059-4
更新日期：2006-04-01 00:00:00
abstract：:Protoplasts were isolated from seedling roots, hypocotyls, and cotyledons of four cultivars of Helianthus annuus and from leaves of axenic shoot cultures of the wild species H. praecox, H. scaberimus and H. rigidus. Optimal culture conditions were established for the respective protoplast systems, using the agarose be...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269815
更新日期：1986-08-01 00:00:00
abstract：:The aim of this study was to optimize the in vitro chromosome-doubling procedure in wheat anther culture. Colchicine, at concentrations of 100-5000 mg/l, was added to the induction medium for 1-5 days. Beneficial effects were obtained with concentrations of 100 and 1000 mg/l colchicine. With time, significant reductio...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050520
更新日期：1998-09-01 00:00:00
abstract：:A collection of 29 pathogenesis-related 10 (PR10) genes of Medicago sativa and Medicago truncatula showed that they were almost all obtained from cDNA libraries of tissues undergoing abiotic or biotic stresses. The predicted proteins could be divided into two subclasses, PR10.1 and PR10.2, but in silico predicted mode...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0909-6
更新日期：2010-11-01 00:00:00
abstract：:The human Bcl-xL gene was transformed into peanut cultivar Georgia Green via microprojectile bombardment. Following selection on hygromycin-containing medium and regeneration, eighty hygromycin-resistant callus clusters were recovered. Southern blot analysis of ten fertile lines revealed multiple insertions of the Bcl...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0444-2
更新日期：2008-01-01 00:00:00
abstract：: Alginate-coated meristems from in vitro-grown axillary buds of mint (Mentha spicata L.) were successfully cryopreserved by vitrification. Excised meristems from nodal segments cold hardened at 4 °C for 3 weeks were encapsulated and osmoprotected by a mixture of 2 M glycerol plus 0.4 M sucrose. These meristems were d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050725
更新日期：1999-12-01 00:00:00
abstract：:Albinism remains a major problem in cereal improvement programs that rely on doubled haploid (DH) technology, and the factors controlling the phenomenon are not well understood. Here we report on the positive influence of copper on the production of DH plants obtained through microspore embryogenesis (ME) in recalcitr...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0733-z
更新日期：2009-09-01 00:00:00
abstract：: This study was conducted to detect the optimum conditions for DNA transfer into mature embryos of barley via electroporation. Cultured mature embryos of barley were directly electroporated in the presence of the pBI 121 vector carrying both the β-glucuronidase (GUS) and neomycin phosphotransferase II (npt II) genes. ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002999900182
更新日期：2000-07-01 00:00:00
abstract：:We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck×Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050311
更新日期：1997-09-01 00:00:00
